γ-聚谷氨酸的研究及应用进展
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摘要
γ-聚谷氨酸是一种生物高分子物质,因其具有良好的水溶性,生物相容性、生物可降解性、无毒性等优点,在医药、食品、化妆品、农业等领域应用广泛,尤其是在肥料中应用价值大,受到广泛关注.本文综述了γ-聚谷氨酸的结构与性质、合成方法、生产菌株、提取纯化、含量测定及应用方面的最新研究进展,为研究同行提供参考,同时为进一步开发应用提供依据.
As a biopolymer substance,γ-polyglutamic acid(γ-PGA) possess good water solubility,biocompatibility,non-degradability and non-toxicity.It is widely used in medicine,food,cosmetics,agriculture and other fields.The research process in structure and properties,synthesis methods,production strains,extraction and purification,determination of content and application of poly-γ-glutamic acid are reviewed in this paper,to provide reference for further research and development.
As a biopolymer substance,γ-polyglutamic acid(γ-PGA) possess good water solubility,biocompatibility,non-degradability and non-toxicity.It is widely used in medicine,food,cosmetics,agriculture and other fields.The research process in structure and properties,synthesis methods,production strains,extraction and purification,determination of content and application of poly-γ-glutamic acid are reviewed in this paper,to provide reference for further research and development.
引文
[1] 景中建.聚谷氨酸的合成方法及应用[J].广东化工,2011,38(2):228-229.
[2] SANDA F,FNJIYAMA T,ENDO T.Chemical synthesis of poly-γ-glutamic acid by condensation of γ-glutamic acid dimer:synthesis and reaction of poly-γ-glutamic acid methyester[J].Polymer Science,2001,39(5):732-741.
[3] 孟杰,卢珂,张轶.酶法快速检测发酵液中的γ-聚谷氨酸浓度[J].食品工业科技,2008,29(9):273-274.
[4] IVANOVICS G.BRUCKNCR V.Chemische und immunologische studien uber den mechanismus der milzbrandinfecktion und immunitat die chemische struktur der kapselsub stanz des milzbrandbazillus und der serolish identischen spezifichen substanz des Bacillus mesentericus[J].Zeitschrift Für Immunit?tsforschung Immunobiology,1937,90(8):304-318.
[5] BOVARNICK M.The formation of extracellular D-glutamic acid polypeptide by Bacillus subtilis[J].Biological Chemistry,1942,145(6):415-424.
[6] 杜丹清,王刚,庹有朋,等.γ-PGA生产菌种的ARTP诱变及其发酵条件优化[J].食品与发酵科技,2017,53(6):5-10.
[7] 冯霞,程金权,王玲,等.γ-聚谷氨酸高产菌株的诱变选育研究[J].食品与发酵科技,2013,49(2):13-16.
[8] 吉美萍.高压电晕电场诱变纳豆芽孢杆菌合成γ-PGA产量提高的研究[D].呼和浩特:内蒙古大学,2017.
[9] 索晨,梅乐,黄俊,等.60Co γ射线诱变选育聚谷氨酸高产菌株及培养基初步优化[J].高校化学工程学报,2007(5):820-825.
[10] 朱凡,吕忠良,杨叶东,等.高黏发酵液中γ-聚谷氨酸的分离纯化工艺[J].化学工程,2013,41(12):9-11.
[11] 杨树峰,楼鹏,李振海,等.聚谷氨酸发酵菌体去除工艺研究[J].食品与发酵科技,2013,49(1):13-16.
[12] 曾庆东,李文杰,王志伟,等.甲醇-乙醇分步沉淀法提取γ-聚谷氨酸[J].食品与发酵工业,2014,40(5):222-228.
[13] 贺杨扬,曾伟,王青龙,等.γ-聚谷氨酸发酵液预处理及提取纯化工艺[J].食品工业科技,2014,35(6):156-160.
[14] DO J H,CHANG H N,LEE S Y.Efficient recovery of poly from highly viscous culture broth[J].Biotechnology and Bioengineering,2001,76(3):219-223.
[15] RAGHAVARAO K S M S,RANGANATHAN T V,SRINIVAS N D,et al.Aqueous two phase extraction an environmentally benign technique[J].Clean Technologies and Environment Policy,2003(5):136-141.
[16] GIRALDO-ZUNIGA A D,COIMBRA J S R,MINIM L A,et al.Axial mixing in a Graesser liquid-liquid contactor using aqueous two-phasesys-tems[J].Chemical Engineering and Processing,2005,44(7):441-446.
[17] 冯志彪,屈玉霄,张晓庆,等.亲水有机溶剂-盐双水相体系分离乳清蛋白抗氧化性多肽[J].中国食品学报,2018,18(10):170-177.
[18] 曾颖,朱新儒,余垒,等.响应面法优化离子液体双水相萃取木瓜蛋白酶[J].食品研究与开发,2018,39(20):29-34.
[19] 马玉,张龙,陈旭升,等.采用双水相萃取技术提取ε-聚赖氨酸[J].食品与发酵工业,2018,44(8):149-156.
[20] 张智维,刘婷,韩飞.正丙醇/碳酸钠双水相体系提取发酵液中γ-聚谷氨酸的研究[J].陕西科技大学学报(自然科学版),2015,33(5):130-134+140.
[21] 王浩,赵祥颖,田延军,等.阴离子交换层析法分离纯化γ-PGA[J].食品工业科技,2012,33(11):253-255+263.
[22] 吕忠良.γ-多聚谷氨酸(γ-PGA)的分离纯化研究[D].杭州:浙江大学,2008.
[23] 纪蓉,江海,胡亚芹,等.γ-聚谷氨酸对带鱼鱼糜凝胶特性的影响[J].中国食品学报,2012,12(4):90-99.
[24] 梁金钟,毕明月,王风青,等.γ-聚谷氨酸在冰激凌中的应用研究[J].农产品加工,2016(23):6-10+13.
[25] 宋佳薇,袁志怡,常忠义,等.γ-聚谷氨酸对冷冻面团和面条的抗冻保护作用[J].食品工业科技,2018,29(31):39-42.
[26] 苏从毅,王辛,王四维,等.提高面包酵母耐冷冻性的研究进展[J].粮食与食品工业,2012,19(6):77-79.
[27] ADITYA R B,VICTOR U I,TERRY B,et al.Bacillus subtilis natto:a non-toxic source of poly-γ-glutamic acid that could be used as acryoprotectant for probiotic bacteria[J].Applied Microbiology and Biotechnology,2013,3(36):2-9.
[28] 赵凯亚,姬晓月,沈亚鹏,等.聚谷氨酸对油条特性与品质的影响[J].河南工业大学学报(自然科学版),2017,38(4):75-80.
[29] 疏秀林,施庆珊,等.γ-聚谷氨酸对荔枝常温货架保鲜效果研究[J].食品工业科技,2012,33(21):318-321+325.
[30] 孙廷丽,施庆珊,欧阳友生,等.聚谷氨酸在医药领域的应用[J].生物技术进展,2012,2(1):34-38.
[31] QI Na,TANG Bo,LIU Guang,et al.Poly(γ-glutamic acid)-coated lipoplexes loaded with doxorubicin for enhancing the antitumor activity against liver tumors[J].Nanoscale Research Letters,2017,12(361):2-9.
[32] SU Y S,LI X,LIU Q Z,et al.Improved poly-γ-glutamicacid production by chromosomal integration of the Vit-reoscilla hemoglobin gene (vgb) in Bacillus subtilis[J].Bioresource Technology,2010,101( 12):4733-4736.
[33] 玉蕾叶,孙洪敏,杜朝,等.聚谷氨酸对小鼠钙吸收的影响[J].华北农学报,2014,29(增刊):202-205.
[34] CHOI H J,YANG R,AND KUNIOKA M.Synthesis and characterization of pH-sensitive and biodegradable hydrogels prepared by gamma irradiation using microbial poly (γ-glutamic acid) and poly (epsilon-lysine)[J].Applied Polymer Science,1995,58(3):807-811.
[35] 孙顶国,邹德龙,王美华,等.γ-聚谷氨酸在冬小麦生产中的应用及肥效研究[J].化肥工业,2014,41(6):74-76.
[36] 陈东义,华振亮,卿树政,等.炭吸附聚谷氨酸有机水溶肥”对玉米田减肥增产的作用[J].农学学报,2017,7(4):25-28.
[37] 褚群,董春娟,尚庆茂.γ-聚谷氨酸对番茄穴盘育苗基质矿质养分供应及幼苗生长发育的影响[J].植物营养与肥料学报,2016,22(3):855-862.
[38] 许宗奇,万传宝,许仙菊,等.肥料增效剂γ-聚谷氨酸对小青菜产量和品质的影响[J].生物加工过程,2012,10(1):58-62.
[39] 王美华.味丹聚谷氨酸及其在农业中的应用[J]中国农资,2015(4):25-25.
[40] 朱安婷.聚γ-谷氨酸缓解水稻干旱、高盐和低温胁迫的生理机制[J].武汉:华中农业大学,2011.
[41] 朱安婷,蒋友武,谢国生,等.外源聚γ-谷氨酸对水稻幼苗耐旱性和渗透调节的影响[J].核农学报,2010,24(6):1269-1273.
[42] 张言芳,周慧梅,蔡克桐,等.无机盐混合试剂与γ-PGA复合处理对早稻苗期耐冷性的引发和分子效应分析[J].农业环境科学学报,2013,32(12):2323-2330.
[43] 唐冬.γ-聚谷氨酸和耐盐植物联合修复设施栽培盐渍化土壤[D].重庆:西南大学,2015.
[44] 蔡志坚.γ-聚谷氨酸活化磷矿粉对Pb污染土壤铅形态及小白菜生长的影响[D].武汉:华中农业大学,2010.
[45] 刘霞,刘飞,刘少英,等.聚谷氨酸的保湿功效及安全性评价[J].日用化学工业,2015,45(5):275-278.
[46] 张天娇,刘霞,邓观杰,等.γ-聚谷氨酸体外抗氧化性及抑制透明质酸酶活性的研究[J].食品与药品,2017,19(3):153-157.
[47] LIU X,LIU F,LIU S,et al.Poly-γ-glutamate from Bacillus subtilis inhibits tyrosinase activity and melanogenesis[J].Applied Microbiology Biotechnology,2013,97(10):9801-9809.
[48] 肖玮,孙智慧,王风青,等.新型食品包装材料γ-聚谷氨酸的酯化改性及降解性能研究进展[J].食品工业科技,2016,37(23):383-386.
[49] XIAO W,SUN Z,LIU J,et al.Study of the influence of preparation conditions of γ-PGA ester as a food packaging material on the biodegradation performance[J].Sciences in Graphic Communication and Packaging,2018,35(7):799-803.
[2] SANDA F,FNJIYAMA T,ENDO T.Chemical synthesis of poly-γ-glutamic acid by condensation of γ-glutamic acid dimer:synthesis and reaction of poly-γ-glutamic acid methyester[J].Polymer Science,2001,39(5):732-741.
[3] 孟杰,卢珂,张轶.酶法快速检测发酵液中的γ-聚谷氨酸浓度[J].食品工业科技,2008,29(9):273-274.
[4] IVANOVICS G.BRUCKNCR V.Chemische und immunologische studien uber den mechanismus der milzbrandinfecktion und immunitat die chemische struktur der kapselsub stanz des milzbrandbazillus und der serolish identischen spezifichen substanz des Bacillus mesentericus[J].Zeitschrift Für Immunit?tsforschung Immunobiology,1937,90(8):304-318.
[5] BOVARNICK M.The formation of extracellular D-glutamic acid polypeptide by Bacillus subtilis[J].Biological Chemistry,1942,145(6):415-424.
[6] 杜丹清,王刚,庹有朋,等.γ-PGA生产菌种的ARTP诱变及其发酵条件优化[J].食品与发酵科技,2017,53(6):5-10.
[7] 冯霞,程金权,王玲,等.γ-聚谷氨酸高产菌株的诱变选育研究[J].食品与发酵科技,2013,49(2):13-16.
[8] 吉美萍.高压电晕电场诱变纳豆芽孢杆菌合成γ-PGA产量提高的研究[D].呼和浩特:内蒙古大学,2017.
[9] 索晨,梅乐,黄俊,等.60Co γ射线诱变选育聚谷氨酸高产菌株及培养基初步优化[J].高校化学工程学报,2007(5):820-825.
[10] 朱凡,吕忠良,杨叶东,等.高黏发酵液中γ-聚谷氨酸的分离纯化工艺[J].化学工程,2013,41(12):9-11.
[11] 杨树峰,楼鹏,李振海,等.聚谷氨酸发酵菌体去除工艺研究[J].食品与发酵科技,2013,49(1):13-16.
[12] 曾庆东,李文杰,王志伟,等.甲醇-乙醇分步沉淀法提取γ-聚谷氨酸[J].食品与发酵工业,2014,40(5):222-228.
[13] 贺杨扬,曾伟,王青龙,等.γ-聚谷氨酸发酵液预处理及提取纯化工艺[J].食品工业科技,2014,35(6):156-160.
[14] DO J H,CHANG H N,LEE S Y.Efficient recovery of poly from highly viscous culture broth[J].Biotechnology and Bioengineering,2001,76(3):219-223.
[15] RAGHAVARAO K S M S,RANGANATHAN T V,SRINIVAS N D,et al.Aqueous two phase extraction an environmentally benign technique[J].Clean Technologies and Environment Policy,2003(5):136-141.
[16] GIRALDO-ZUNIGA A D,COIMBRA J S R,MINIM L A,et al.Axial mixing in a Graesser liquid-liquid contactor using aqueous two-phasesys-tems[J].Chemical Engineering and Processing,2005,44(7):441-446.
[17] 冯志彪,屈玉霄,张晓庆,等.亲水有机溶剂-盐双水相体系分离乳清蛋白抗氧化性多肽[J].中国食品学报,2018,18(10):170-177.
[18] 曾颖,朱新儒,余垒,等.响应面法优化离子液体双水相萃取木瓜蛋白酶[J].食品研究与开发,2018,39(20):29-34.
[19] 马玉,张龙,陈旭升,等.采用双水相萃取技术提取ε-聚赖氨酸[J].食品与发酵工业,2018,44(8):149-156.
[20] 张智维,刘婷,韩飞.正丙醇/碳酸钠双水相体系提取发酵液中γ-聚谷氨酸的研究[J].陕西科技大学学报(自然科学版),2015,33(5):130-134+140.
[21] 王浩,赵祥颖,田延军,等.阴离子交换层析法分离纯化γ-PGA[J].食品工业科技,2012,33(11):253-255+263.
[22] 吕忠良.γ-多聚谷氨酸(γ-PGA)的分离纯化研究[D].杭州:浙江大学,2008.
[23] 纪蓉,江海,胡亚芹,等.γ-聚谷氨酸对带鱼鱼糜凝胶特性的影响[J].中国食品学报,2012,12(4):90-99.
[24] 梁金钟,毕明月,王风青,等.γ-聚谷氨酸在冰激凌中的应用研究[J].农产品加工,2016(23):6-10+13.
[25] 宋佳薇,袁志怡,常忠义,等.γ-聚谷氨酸对冷冻面团和面条的抗冻保护作用[J].食品工业科技,2018,29(31):39-42.
[26] 苏从毅,王辛,王四维,等.提高面包酵母耐冷冻性的研究进展[J].粮食与食品工业,2012,19(6):77-79.
[27] ADITYA R B,VICTOR U I,TERRY B,et al.Bacillus subtilis natto:a non-toxic source of poly-γ-glutamic acid that could be used as acryoprotectant for probiotic bacteria[J].Applied Microbiology and Biotechnology,2013,3(36):2-9.
[28] 赵凯亚,姬晓月,沈亚鹏,等.聚谷氨酸对油条特性与品质的影响[J].河南工业大学学报(自然科学版),2017,38(4):75-80.
[29] 疏秀林,施庆珊,等.γ-聚谷氨酸对荔枝常温货架保鲜效果研究[J].食品工业科技,2012,33(21):318-321+325.
[30] 孙廷丽,施庆珊,欧阳友生,等.聚谷氨酸在医药领域的应用[J].生物技术进展,2012,2(1):34-38.
[31] QI Na,TANG Bo,LIU Guang,et al.Poly(γ-glutamic acid)-coated lipoplexes loaded with doxorubicin for enhancing the antitumor activity against liver tumors[J].Nanoscale Research Letters,2017,12(361):2-9.
[32] SU Y S,LI X,LIU Q Z,et al.Improved poly-γ-glutamicacid production by chromosomal integration of the Vit-reoscilla hemoglobin gene (vgb) in Bacillus subtilis[J].Bioresource Technology,2010,101( 12):4733-4736.
[33] 玉蕾叶,孙洪敏,杜朝,等.聚谷氨酸对小鼠钙吸收的影响[J].华北农学报,2014,29(增刊):202-205.
[34] CHOI H J,YANG R,AND KUNIOKA M.Synthesis and characterization of pH-sensitive and biodegradable hydrogels prepared by gamma irradiation using microbial poly (γ-glutamic acid) and poly (epsilon-lysine)[J].Applied Polymer Science,1995,58(3):807-811.
[35] 孙顶国,邹德龙,王美华,等.γ-聚谷氨酸在冬小麦生产中的应用及肥效研究[J].化肥工业,2014,41(6):74-76.
[36] 陈东义,华振亮,卿树政,等.炭吸附聚谷氨酸有机水溶肥”对玉米田减肥增产的作用[J].农学学报,2017,7(4):25-28.
[37] 褚群,董春娟,尚庆茂.γ-聚谷氨酸对番茄穴盘育苗基质矿质养分供应及幼苗生长发育的影响[J].植物营养与肥料学报,2016,22(3):855-862.
[38] 许宗奇,万传宝,许仙菊,等.肥料增效剂γ-聚谷氨酸对小青菜产量和品质的影响[J].生物加工过程,2012,10(1):58-62.
[39] 王美华.味丹聚谷氨酸及其在农业中的应用[J]中国农资,2015(4):25-25.
[40] 朱安婷.聚γ-谷氨酸缓解水稻干旱、高盐和低温胁迫的生理机制[J].武汉:华中农业大学,2011.
[41] 朱安婷,蒋友武,谢国生,等.外源聚γ-谷氨酸对水稻幼苗耐旱性和渗透调节的影响[J].核农学报,2010,24(6):1269-1273.
[42] 张言芳,周慧梅,蔡克桐,等.无机盐混合试剂与γ-PGA复合处理对早稻苗期耐冷性的引发和分子效应分析[J].农业环境科学学报,2013,32(12):2323-2330.
[43] 唐冬.γ-聚谷氨酸和耐盐植物联合修复设施栽培盐渍化土壤[D].重庆:西南大学,2015.
[44] 蔡志坚.γ-聚谷氨酸活化磷矿粉对Pb污染土壤铅形态及小白菜生长的影响[D].武汉:华中农业大学,2010.
[45] 刘霞,刘飞,刘少英,等.聚谷氨酸的保湿功效及安全性评价[J].日用化学工业,2015,45(5):275-278.
[46] 张天娇,刘霞,邓观杰,等.γ-聚谷氨酸体外抗氧化性及抑制透明质酸酶活性的研究[J].食品与药品,2017,19(3):153-157.
[47] LIU X,LIU F,LIU S,et al.Poly-γ-glutamate from Bacillus subtilis inhibits tyrosinase activity and melanogenesis[J].Applied Microbiology Biotechnology,2013,97(10):9801-9809.
[48] 肖玮,孙智慧,王风青,等.新型食品包装材料γ-聚谷氨酸的酯化改性及降解性能研究进展[J].食品工业科技,2016,37(23):383-386.
[49] XIAO W,SUN Z,LIU J,et al.Study of the influence of preparation conditions of γ-PGA ester as a food packaging material on the biodegradation performance[J].Sciences in Graphic Communication and Packaging,2018,35(7):799-803.