高效液相色谱法同时测定苹果醋及原料中的17种有机酸
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摘要
建立了高效液相色谱法同时测定苹果醋及原料中17种有机酸的测定方法。样品经LC-C_(18)SPE固相萃取柱净化后,采用InertsilRODS-3色谱柱(250mm×4.6mm,5μm)进行分离,流动相为0.01mol/L(NH_4)_2HPO_4(pH2.45),流速为0.5mL/min,在紫外检测波长210nm处,可以实现17种有机酸的分离和准确定量。该方法回收率74.3%~107.3%,相对标准偏差(RSD)2.0%~7.0%(n=6),各有机酸的线性相关系数R2>0.998,具有简便、快捷、准确性高等优点。采用该方法对苹果醋及其原料浓缩苹果汁、苹果酒中有机酸组成及含量进行比较发现,三者在苹果酸、乳酸、乙酸含量上存在显著差异(P<0.05)。
The simultaneous determination of 17 organic acids in apple vinegar and raw materials was established by HPLC. The sample was purified by LC-C_(18)SPE column and separated by InertsilRODS-3 column(250mm×4.6mm,5μm), at the condition of mobile phase 0.01mol/L((NH_4)_2HPO_4(pH2.45) with flow rate of 0.5 ml/min, and detection wavelength 210 nm, the 17 organic acids could be separated and quantitatively determined. The recovery rates of the method were 74.3%-107.3% with the relative standard deviations(RSD) of 2.0%-7.0%(n=6). The correlation coefficients(R2)of 17 organic acids were more than 0.998. The results indicated that the method was simple, rapid and accurate. With this method, the organic acids composition and concentrations in apple vinegar, concentrated apple juice and apple cider were compared, and their malic acid, lactic acid and acetic acid contents showed significant differences(P<0.05).
The simultaneous determination of 17 organic acids in apple vinegar and raw materials was established by HPLC. The sample was purified by LC-C_(18)SPE column and separated by InertsilRODS-3 column(250mm×4.6mm,5μm), at the condition of mobile phase 0.01mol/L((NH_4)_2HPO_4(pH2.45) with flow rate of 0.5 ml/min, and detection wavelength 210 nm, the 17 organic acids could be separated and quantitatively determined. The recovery rates of the method were 74.3%-107.3% with the relative standard deviations(RSD) of 2.0%-7.0%(n=6). The correlation coefficients(R2)of 17 organic acids were more than 0.998. The results indicated that the method was simple, rapid and accurate. With this method, the organic acids composition and concentrations in apple vinegar, concentrated apple juice and apple cider were compared, and their malic acid, lactic acid and acetic acid contents showed significant differences(P<0.05).
引文
[1]熊贤平,高洪波,罗培余.双柱串联反相高效液相色谱测定苹果醋中的有机酸[J].食品工业科技,2016,37(21):299-303.
[2]李军鹏,付彩霞,王应喜,等.蜜柑果醋的风味物质成分分析[J].中国酿造,2015,34(11):154-157.
[3]姚玉静,龚慧雯,王尔茂.果醋的保健功能[J].综述与评述,2009,12(9):1-2.
[4]张奇,叶正良,李德坤,等.电位滴定法测定五味子提取物中总有机酸的含量[J].陕西中医,2011,32(3):342-343.
[5]牛金刚,梁晓静,刘霞,等.分光光度法测定水溶液中的有机酸含量[J].应用化学,2010,27(3):342-346.
[6]戴达勇,杨英桂,贺万鑫.离子色谱法同时测定沙棘果粉中7种有机酸含量[J].理化检测(化学分册),2015,51(3):304-306.
[7]王贵双,唐坤甜,鲁绯,等.离子交换色谱法测定食醋中有机酸[J].中国酿造,2010,29(7):166-168.
[8]张伟,李晓君,张静,等.柱前衍生-气相色谱法同时测定功能食品中8种有机酸[J].分析试验室,2014,33(2):175-179.
[9]张霞,刘志华,杨光宇,等.固相萃取富集/气相色谱法测定烟草中的9种有机酸[J].分析测试学报,2014,33(5):545-550.
[10]郭燕,梁俊,李敏敏,等.高效液相色谱法测定苹果果实中的有机酸[J].食品科学,2012,33(2):227-230.
[11]薛霞,霍艳敏,王骏,等.固相萃取-高效液相色谱法测定果醋中的7种有机酸[J].食品科学,2012,33(14):247-250.
[12]徐玉涛,李珂珂,王贺新,等.高效液相色谱法对蓝莓果实中8个有机酸含量的测定[J].食品科学,2015,36(18):127-131.
[13]黄桂颖,白卫东,杨幼慧,等.反相高效液相色谱法测定荔枝肉中10种有机酸[J].现代食品科技,2009,25(5):568-570.
[14]高海燕,廖小军,王善广,等.反相高效液相色谱法测定果汁中11种有机酸条件的优化[J].分析化学研究简报,2004,32(12):1645-1648.
[15]王琳,陈双,徐岩.固相萃取-超高效液相色谱法测定黄酒中的有机酸[J].食品与发酵工业,2016,42(10):142-148.
[16]杨毅,李崎,陈蕴,等.反相高效液相色谱法(RP-HPLC)测定啤酒中有机酸[J].食品与发酵工业,2003,29(8):6-12.
[17]余永建,邓晓阳,陆震鸣,等.固态发酵食醋有机酸组成分析中样品预处理方法的研究[J].食品工业科技,2013,34(4):198-201.
[2]李军鹏,付彩霞,王应喜,等.蜜柑果醋的风味物质成分分析[J].中国酿造,2015,34(11):154-157.
[3]姚玉静,龚慧雯,王尔茂.果醋的保健功能[J].综述与评述,2009,12(9):1-2.
[4]张奇,叶正良,李德坤,等.电位滴定法测定五味子提取物中总有机酸的含量[J].陕西中医,2011,32(3):342-343.
[5]牛金刚,梁晓静,刘霞,等.分光光度法测定水溶液中的有机酸含量[J].应用化学,2010,27(3):342-346.
[6]戴达勇,杨英桂,贺万鑫.离子色谱法同时测定沙棘果粉中7种有机酸含量[J].理化检测(化学分册),2015,51(3):304-306.
[7]王贵双,唐坤甜,鲁绯,等.离子交换色谱法测定食醋中有机酸[J].中国酿造,2010,29(7):166-168.
[8]张伟,李晓君,张静,等.柱前衍生-气相色谱法同时测定功能食品中8种有机酸[J].分析试验室,2014,33(2):175-179.
[9]张霞,刘志华,杨光宇,等.固相萃取富集/气相色谱法测定烟草中的9种有机酸[J].分析测试学报,2014,33(5):545-550.
[10]郭燕,梁俊,李敏敏,等.高效液相色谱法测定苹果果实中的有机酸[J].食品科学,2012,33(2):227-230.
[11]薛霞,霍艳敏,王骏,等.固相萃取-高效液相色谱法测定果醋中的7种有机酸[J].食品科学,2012,33(14):247-250.
[12]徐玉涛,李珂珂,王贺新,等.高效液相色谱法对蓝莓果实中8个有机酸含量的测定[J].食品科学,2015,36(18):127-131.
[13]黄桂颖,白卫东,杨幼慧,等.反相高效液相色谱法测定荔枝肉中10种有机酸[J].现代食品科技,2009,25(5):568-570.
[14]高海燕,廖小军,王善广,等.反相高效液相色谱法测定果汁中11种有机酸条件的优化[J].分析化学研究简报,2004,32(12):1645-1648.
[15]王琳,陈双,徐岩.固相萃取-超高效液相色谱法测定黄酒中的有机酸[J].食品与发酵工业,2016,42(10):142-148.
[16]杨毅,李崎,陈蕴,等.反相高效液相色谱法(RP-HPLC)测定啤酒中有机酸[J].食品与发酵工业,2003,29(8):6-12.
[17]余永建,邓晓阳,陆震鸣,等.固态发酵食醋有机酸组成分析中样品预处理方法的研究[J].食品工业科技,2013,34(4):198-201.