核酸疫苗免疫机制研究新进展
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摘要
核酸疫苗与传统疫苗相比具有不可比拟的优势,可诱导机体产生细胞免疫和体液免疫,其免疫保护作用主要通过MHCⅠ类分子途径、MHCⅡ类分子途径和B细胞途径等3种途径来实现。通过MHCⅠ类分子途径激活CD8~+T细胞,产生特异的CTL效应细胞免疫;通过MHCⅡ类分子途径激活CD4~+T细胞,分泌IL-2、INF-γ、TNF-β等细胞因子,辅助机体产生特异性体液免疫;通过B细胞途径刺激并活化B细胞,诱导B细胞增殖分化,产生大量特异性抗体,诱发体液免疫应答。
引文
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[11]肖跃强,管宇,谢金文,等.狂犬病“靶向性”Th表位DNA疫苗的构建与瞬时表达研究[J].中国兽医科学,2012(2):176-181.
[12]肖跃强,谢金文,李书光,等.狂犬病"靶向性"中和表位DNA疫苗的构建与瞬时表达[J].中国兽医学报,2013,33(7):1021-1026.
[13]谭磊.流感H3/H1亚型多表位核酸疫苗和重组腺病毒疫苗构建及实验免疫研究[D].长春:吉林大学,2011.
[14]TAN L,LU H J,ZHANG D,et al.Efficacy of seasonal pandemic influenza hemagglutinin DNA vaccines delivered by electroporation against a seasonal H1N1 virus challenge in mice[J].Sci China Life Sci,2011,54(4):293-299.
[15]WEI C J,BOYINGTON J C,MCTAMNEY P M,et al.Induction of broadly neutralizing H1N1 influenza antibodies by vaccination[J].Science,2010,329(5995):1060-1064.
[2]WOLFF J A,LUDTKE J J,ACSADI G,et al.Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle[J].Hum Mol Genet,1992,1(6):363-369.
[3]TANG D C,DEVIT M,JOHNSTON S A.Genetic immunization is a simple method for eliciting an immune response[J].Nature,1992,356(6365):152-154.
[4]INGOLOTTI M,KAWALEKAR O,SHEDLOCK D J,et al.DNA vaccines for targeting bacterial infections[J].Expert Rev Vaccines,2010,9(7):747-763.
[5]YANKAUCKAS M A,MORROW J E,PARKER S E,et al.Long-term anti-nucleoprotein cellular and humoral immunity is induced by intramuscular injection of plasmid DNA containing NP gene[J].DNA Cell Biol,1993,12(9):771-776.
[6]陈利红.核酸疫苗在寄生虫疾病防治中的应用[J].黔南民族医专学报,2004,17(3):183-185.
[7]KUTZLER M A,WEINER D B.DNA vaccines:ready for prime time?[J].Nat Rev Genet,2008,9(10):776-788.
[8]何向锋,王净,余方流,等.GPI修饰的ESAT-6核酸疫苗的构建及其免疫功能初探[J].免疫学杂志,2011(9):737-740.
[9]靖杰,鲁会军,刘昊,等.Quil A为佐剂的H1、H3亚型流感病毒多表位DNA疫苗免疫评价[J].中国病原生物学杂志,2013,8(1):1-4.
[10]沈宪文,王中华,高磊,等.PRRSV GP5蛋白及猪IL-18基因共表达核酸疫苗免疫原性试验[J].中国兽医学报,2011,31(11):1543-1549.
[11]肖跃强,管宇,谢金文,等.狂犬病“靶向性”Th表位DNA疫苗的构建与瞬时表达研究[J].中国兽医科学,2012(2):176-181.
[12]肖跃强,谢金文,李书光,等.狂犬病"靶向性"中和表位DNA疫苗的构建与瞬时表达[J].中国兽医学报,2013,33(7):1021-1026.
[13]谭磊.流感H3/H1亚型多表位核酸疫苗和重组腺病毒疫苗构建及实验免疫研究[D].长春:吉林大学,2011.
[14]TAN L,LU H J,ZHANG D,et al.Efficacy of seasonal pandemic influenza hemagglutinin DNA vaccines delivered by electroporation against a seasonal H1N1 virus challenge in mice[J].Sci China Life Sci,2011,54(4):293-299.
[15]WEI C J,BOYINGTON J C,MCTAMNEY P M,et al.Induction of broadly neutralizing H1N1 influenza antibodies by vaccination[J].Science,2010,329(5995):1060-1064.