HPV E6/E7 mRNA联合HPV L1衣壳蛋白在子宫颈癌前病变诊断中的价值
摘要
目的探讨HPV E6/E7 mRNA联合HPV L1衣壳蛋白检测在子宫颈癌前病变诊断中的价值。方法采用分支链DNA信号放大技术检测156例子宫颈活检不同病变组织中HPV E6/E7 mRNA的表达,并应用免疫组化En Vision法检测HPV L1衣壳蛋白的表达。结果与正常组织相比,子宫颈低级别上皮内病变(low-grade squamous intraepithelial lesion,LSIL)、子宫颈高级别上皮内病变(high-grade squamous intraepithelial lesion,HSIL)和子宫颈鳞癌组织中HPV E6/E7mRNA的阳性率增高(P <0. 05),且HSIL和子宫颈癌中HPV E6/E7 mRNA的阳性率高于LSIL(P <0. 05),但HSIL和子宫颈癌组织中HPV E6/E7 mRNA的阳性率差异无显著性(P> 0. 05); HPV L1衣壳蛋白在LSIL中的阳性率明显高于HSIL(P <0. 05)。结论通过对子宫颈不同病变标本进行HPV E6/E7 mRNA和HPV L1衣壳蛋白联合检测,了解不同病变子宫颈组织中HPV E6/E7 mRNA和HPV L1衣壳蛋白的表达趋势,可预测子宫颈上皮内病变的发展趋势,指导临床医师进行合理治疗。
引文
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[13]Rauber D,Mehlhoru G,Fasching P A,et al.Prognostic significance of the detection of human papilloma virus L1 protein in smears of mild to moderate cervical intraepithelial lesions[J].Eur J Obstet Gynecol Reprod Biol,2008,140(2):258-262.
[14]Liu C,Du H,Wang C,et al.HPV L1 and P16 expression in CIN1to predict future CIN2[J].Int J Gynecol Pathol,2017,36(3):281-288.
[15]王家建,田其芳,张甦,等.高危型HPV阳性妇女子宫颈脱蜡细胞HPV L1蛋白检测的临床意义[J].中华妇产科杂志,2015,50(4):253-257.
[2]Melsheimer P,Kaui S,Dobeck S,et al.Immunocytochemical detection of HPV high-risk type L1 capsid proteins in LSIL and HSILas compared with detection of HPV L1 DNA[J].Acta Cytol,2003,47(2):124-128.
[3]Torre L A,Bray F,Siegel R L,et al.Global cancer statistics,2015[J].CA Cancer J Clin,2015,65(2):87-108.
[4]Scheurer M E,Tortdero L G,Adler S K.Human papillomavirus infection:biology,epidemiology and prevention[J].Int J Gynecol Cancer,2005,15(5):727-746.
[5]Skiba D,Mehlhorn G,Fasching P A,et al.Prognostic significance of serum antibodies to HPV-16 L1 virus-like particles in patients with invasive cervical cancer[J].Anticancer Res,2006,26(6C):4921-4926.
[6]Hoppe-Seyler K,Bossler F,Braun J A,et al.The HPV E6/E7oncogenes:key factors for viral carcinogenesis and therapeutic targets[J].Trends Microbiol,2018,26(2):158-168.
[7]De Filippis R A,Goodwin E C,Wu L,Di Maio D.Endogenous human papillomavims E6 and E7 proteins differentially regulate proliferation senescence and apoptosis in He La cervical carcinoma cells[J].J Virol,2003,77(2):1551-1563.
[8]郑智,曹晓恩,张红萍.高危型HPV E6/E7 mRNA检测对高级别子宫颈病变的诊断价值[J].浙江医学,2016,38(14):1154-1157.
[9]Shi W J,Liu H,Wu D,et al.E6/E7 proteins are potential markers for the screening and diagnosis of cervical precancerous lesions and cervical cancer in a Chinese poulation[J].Oncol Lett,201714(5):6251-6258.
[10]吕秀芳,宋晓霞,贺慧,等.HPV DNA E6/E7 mRNA在子宫颈病变及子宫颈鳞状细胞癌中的表达[J].临床与实验病理学杂志,2016,32(7):745-748.
[11]Hilfrich R,Hariri J.Prognostic relevance of human papillomavirus L1 capsid protein detection within mild and moderate dysplastic lesions of the cervix uteri in combination with p16 biomarker[J].A-nal Quant Cytol Histol,2008,30(2):78-82.
[12]Yang-Chun F,Yuan Z,Cheng-Ming L,et al.Increased HPV L1gene methylation and multiple infection status lead to the difference of cervical epithelial cell lesion in different ethnic women of Xinjiang,China[J].Medcine(Baltimore),2017,96(12):e6409.
[13]Rauber D,Mehlhoru G,Fasching P A,et al.Prognostic significance of the detection of human papilloma virus L1 protein in smears of mild to moderate cervical intraepithelial lesions[J].Eur J Obstet Gynecol Reprod Biol,2008,140(2):258-262.
[14]Liu C,Du H,Wang C,et al.HPV L1 and P16 expression in CIN1to predict future CIN2[J].Int J Gynecol Pathol,2017,36(3):281-288.
[15]王家建,田其芳,张甦,等.高危型HPV阳性妇女子宫颈脱蜡细胞HPV L1蛋白检测的临床意义[J].中华妇产科杂志,2015,50(4):253-257.