不同维生素浸种对玉米种子萌发的影响
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摘要
以玉米杂交种郑单958为试验材料,采用不同质量浓度50、100、200 mg/L的水溶性维生素V_(B1)、V_(B2)、V_(B6)、V_(B12)、V_C、V_H、V_(PP)浸种24 h,处理后分析玉米种子萌发及胚芽和根生长中脯氨酸等可溶性调节物质情况。结果表明,供试维生素在100、200 mg/L处理下基本都呈现对种子萌发指数的促进,其中,100 mg/L维生素处理对种子萌发率促进效果显著;200 mg/L维生素处理对种子发芽势显著促进作用;50 mg/L维生素对上述3个指标的影响不同。与未处理对照相比,不同种类维生素和不同质量浓度的同一维生素对根冠比影响不同。维生素浸种均可引起玉米胚芽可溶性糖含量显著升高,外源维生素浸种处理可显著影响玉米种子萌发和胚芽及胚根的生长,且不同维生素在不同浓度下对各生长指标影响及脯氨酸等可溶性调节物质变化不尽相同,对种子萌发呈显著促进作用的V_(B2)和V_(B12)在玉米种子萌发中应用前景较好。
In this study, we used six kinds of exogenous vitamin(V_(B1), V_(B2), V_(B6), V_(B12), V_C, V_H and V_(PP)) to tested their effects on seed germination. For each vitamin, we soaked the seeds of maize hybrid Zhengdan958 for 24 h at 50, 100 and 200 mg/L, respectively. The seed germination rate and the contents of proline and other soluble sub-stances in plumule and radicle were analyzed. All the tested vitamins at 100 mg/L and 200 mg/L would promote seed gemination, with 100 mg/L and 200 mg/L vitamin solutions significantly increased seed germination rate and seed germination potential, respectively. While 50 mg/L vitamin solutions influenced the indexes of seed gemina-tion in different ways. In addition, different vitamins had different effects on the root-shoot ratio of seedlings, with V_(B1), V_(B2) and V_(B12) solutions had promotion effects while V_(B2) solutions had inhibition effects. All vitamin solutions could increase the soluble sugar content significantly, while they acted on proline in different ways. In total, exoge-nous vitamins could significantly affect maize seed germination such as plumule and radicle growth, while the ef-fects of different vitamins were different. Among them, V_(B2) and V_(B12) had the best effect on seed germination.
In this study, we used six kinds of exogenous vitamin(V_(B1), V_(B2), V_(B6), V_(B12), V_C, V_H and V_(PP)) to tested their effects on seed germination. For each vitamin, we soaked the seeds of maize hybrid Zhengdan958 for 24 h at 50, 100 and 200 mg/L, respectively. The seed germination rate and the contents of proline and other soluble sub-stances in plumule and radicle were analyzed. All the tested vitamins at 100 mg/L and 200 mg/L would promote seed gemination, with 100 mg/L and 200 mg/L vitamin solutions significantly increased seed germination rate and seed germination potential, respectively. While 50 mg/L vitamin solutions influenced the indexes of seed gemina-tion in different ways. In addition, different vitamins had different effects on the root-shoot ratio of seedlings, with V_(B1), V_(B2) and V_(B12) solutions had promotion effects while V_(B2) solutions had inhibition effects. All vitamin solutions could increase the soluble sugar content significantly, while they acted on proline in different ways. In total, exoge-nous vitamins could significantly affect maize seed germination such as plumule and radicle growth, while the ef-fects of different vitamins were different. Among them, V_(B2) and V_(B12) had the best effect on seed germination.
引文
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[18]Bonifas K D,Walters D T,Cassman K G.Nitrogen supply affects root:shoot ratio in corn and velvetleaf(Abutilon theophrasti)[M].Agronomy-Faculty Publications.2005:670-675.
[19]Couée I,Sulmon C,Gouesbet G,et al.Involvement of soluble sug-ars in reactive oxygen species balance and responses to oxidative stress in plants[J].Journal of Experimental Botany,2006,57(3):449-459.
[20]Miyake C,Asada K.Ferredoxin-Dependent photoreduction of the monodehydroascorbate radical in spinach thylakoids[J].Plant&Cell Physiology,1994,35(4):539-549.
[2]Asada K.Ascorbate peroxidase-a hydrogen peroxide-scavenging en-zyme in plants[J].Physiologia Plantarum,1992,85(2):235-241.
[3]Prasad A S.Discovery of human zinc deficiency:50 years later[J].Journal of Trace Elements in Medicine&Biology Organ of the Soci-ety for Minerals&Trace Elements,2012,26(2-3):66.
[4]Miret J A,Munnébosch S.Plant amino acid-derived vitamins:bio-synthesis and function[J].Amino Acids,2014,46(4):809-824.
[5]Sattler S E,Gilliland L U,Magallaneslundback M.Vitamin E is es-sential for seed longevity and for preventing lipid peroxidation dur-ing germination[J].Plant Cell,2004,16(6):1419-1432.
[6]Varshney S,Varshney C.Effect of low levels of SO2on glutamate de-hydrogenase isoenzymes in crop plants[J].Biochemie Und Physiolo-gie Der Pflanzen,1984,179(5):433-437.
[7]李振轮,何凯,石纹豪.外源维生素浸种对甜玉米种子萌发和幼苗生长的影响[J].西北农林科技大学学报(自然科学版),2014,42(6):48-54.Li Z L,He K,Shi W H.Effect of seed soaking with exogenous vita-min on seed germination and seedling growth of sweet corn[J].Jour-nal of Northwest A&F University(Natural Science Edition),2014,42(6):48-54.(in Chinese)
[8]Tavili A.Hydropriming,ascorbic and salicylic acid influence on ger-mination of Agropyron elongatum Host.seeds under salt stress[J].Research Journal of Seed Science,2010,3(4):249-255.
[9]Khan M A.Effect of germination regulating chemicals on seed germi-nation of halogeton glomeratus for alleviation of salinity stress[J].Pakistan Journal of Botany,2009,41(4):1205-1212.
[10]Abdallah Atia,Ahmed Debez,Zouhaier Barhoumi,et al.ABA,GA3,and nitrate may control seed germination of crithmum mariti-mum(Apiaceae)under saline conditions[J].Comptes Rendus Biolo-gies,2009,332(8):704-710.
[11]冯文新,张宝红.钙处理对盐胁迫下大豆种子萌发及其生理生化指标的影响[J].大豆科学,1997,16(1):48-53.Feng W X,Zhang B H.Effects of calcium treatment on germinating and physiological I ndexes of soybean seeds under syress of salt[J].Soybean Science,1997,16(1):48-53.(in Chinese)
[12]郭素枝,张育松,陈洪德.赤霉素及矿质元素对茶树活性氧代谢、质膜功能和化学品质的效应[J].福建农林大学学报(自然科学版),1994(3):275-279.Guo S Z,Zhang Y S,Chen H D.Effects of gibberellics and mineral elements on activated oxygen metabolism,function of plasma mem-brane and chemical quality of tea[J].Journal of Fujian Agriculture and Forestry University,1994(3):275-279.(in Chinese)
[13]Keunen E,Peshev D,Vangronsveld J.Plant sugars are crucial play-ers in the oxidative challenge during abiotic stress:extending the traditional concept[J].Plant Cell&Environment,2013,36(7):1242-1255.
[14]Shahba M A,Qian Y L,Lair K D.Improving seed germination of saltgrass under saline conditions[J].Crop Science,2008,48(2):756-762.
[15]Bates L S,Waldren R P,Teare I D.Rapid determination of free pro-line for water-stress studies[J].Plant Soil,1973,39:205-207.
[16]Agren G?ran I,Franklin Oskar.Root:shoot ratios,optimization and nitrogen productivity[J].Annals of Botany,2003,92(6):795-800.
[17]Bloom A J,Chapin F S,Mooney H A.Resource limitation in Plants-An economic analogy[J].Annual Review of Ecology&Sys-tematics,1985,16(1):363-392.
[18]Bonifas K D,Walters D T,Cassman K G.Nitrogen supply affects root:shoot ratio in corn and velvetleaf(Abutilon theophrasti)[M].Agronomy-Faculty Publications.2005:670-675.
[19]Couée I,Sulmon C,Gouesbet G,et al.Involvement of soluble sug-ars in reactive oxygen species balance and responses to oxidative stress in plants[J].Journal of Experimental Botany,2006,57(3):449-459.
[20]Miyake C,Asada K.Ferredoxin-Dependent photoreduction of the monodehydroascorbate radical in spinach thylakoids[J].Plant&Cell Physiology,1994,35(4):539-549.