福州地区碳青霉烯类耐药鲍曼不动杆菌碳青霉烯酶基因型研究
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摘要
目的探讨福州地区碳青霉烯类耐药鲍曼不动杆菌(CRAB)碳青霉烯酶基因型的流行情况。方法收集多家医院临床标本中分离得到的107株CRAB。应用K-B纸片扩散法进行药物敏感试验。采用PCR法检测7种碳青霉烯酶基因,包括OXA-23、OXA-24、OXA-51、OXA-58、IMP-1、IMP-4和VIM-2。结果 107株CRAB对除多粘菌素B、米诺环素外的其他所有常见的抗生素均为耐药。碳青霉烯酶基因OXA-51、OXA-23的检出率分别为100.0%(107/107)和87.9%(94/107)。其他OXA-24、OXA-58、IMP-1、IMP-4和VIM-2基因均未检出。结论福州地区临床分离的CRAB耐药现象严重;表达OXA-23基因是CRAB对碳青霉烯类药物耐药的重要机制之一。
Objective To investigate the prevalence of carbapenemases genotypes in carbapenem-resistant Acinetobacter baumannii(CRAB)in Fuzhou area.Methods Totally 107 strains of CRAB were collected from a number of hospitals in Fuzhou.Drug sensitivity test was performed by using Kirby-Bauer diffusion method.PCR was used to detect seven kinds of carbapenemases genes,including OXA-23,OXA-24,OXA-51,OXA-58,IMP-1,IMP-4and VIM-2.Results The 107 strains of CRAB were resistant to almost all the common antibiotics except Polymyxin B and Minocycline.The detection rates of OXA-51 and OXA-23were100.0%(107/107)and 87.9%(94/107),respectively.OXA-24,OXA-58,IMP-1,IMP-4and VIM-2genes were not detected.Conclusion The resistance of clinical CRAB isolates in Fuzhou area is high.The expression of OXA-23 gene is one of the important mechanisms of their drug resistance to carbapenems.
Objective To investigate the prevalence of carbapenemases genotypes in carbapenem-resistant Acinetobacter baumannii(CRAB)in Fuzhou area.Methods Totally 107 strains of CRAB were collected from a number of hospitals in Fuzhou.Drug sensitivity test was performed by using Kirby-Bauer diffusion method.PCR was used to detect seven kinds of carbapenemases genes,including OXA-23,OXA-24,OXA-51,OXA-58,IMP-1,IMP-4and VIM-2.Results The 107 strains of CRAB were resistant to almost all the common antibiotics except Polymyxin B and Minocycline.The detection rates of OXA-51 and OXA-23were100.0%(107/107)and 87.9%(94/107),respectively.OXA-24,OXA-58,IMP-1,IMP-4and VIM-2genes were not detected.Conclusion The resistance of clinical CRAB isolates in Fuzhou area is high.The expression of OXA-23 gene is one of the important mechanisms of their drug resistance to carbapenems.
引文
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[14]FAN Xuecai,WANG Xiaohong,ZHANG Jisheng,et al.Detection of carbapenem gene in Acinetobacter baumannii from Eastern Heilongjiang province[J].Chin J Microecol,2015,27(6):651-657.(in Chinese)范学财,王晓红,张吉生,等.黑龙江省东部地区鲍曼不动杆菌碳青霉烯酶基因检测[J].中国微生态学杂志,2015,27(6):651-657.
[2]WANG Hui,REN Jiankang,WANG Minggui.Clinical microbiological examination[M].Beijing:People′s Medical Publishing House,2015:417-418.(in Chinese)王辉,任健康,王明贵.临床微生物学检验[M].北京:人民卫生出版社,2015:417-418.
[3]ZHANG Hui,ZHANG Xiaojiang,XU Yingchun,et al.Chinet 2011surveillance of antibiotic resistance in A.baumannii isolates in china[J].Chin J Infect&Chemother,2013,13(5):342-348.(in Chinese)张辉,张小江,徐英春,等.2011年中国CHINET不动杆菌属细菌耐药性监测[J].中国感染与化疗杂志,2013,13(5):342-348.
[4]ZHANG Hui,ZHANG Xiaojiang,XU Yingchun,et al.Chinet 2012surveillance of antibiotic resistance in Acinetobacter baumannii isolates in china[J].Chin J Infect&Chemother,2014,14(5):392-397.(in Chinese)张辉,张小江,徐英春,等.2012年中国CHINET不动杆菌属细菌耐药性监测[J].中国感染与化疗杂志,2014,14(5):392-397.
[5]HU Qiaojuan,HU Zhidong.Research progress of multi-drug resistant Acinetobacter baumannii[J].Int J Lab Med,2010,31(10):1121-1123.(in Chinese)胡巧娟,胡志东.多重耐药鲍曼不动杆菌的研究进展[J].国际检验医学杂志,2010,31(10):1121-1123.
[6]Perez F,Hujer AM,Hujer KM,et al.Global challenge of multidrug-resistant Acinetobacter baumannii[J].Antimicrob Agents Chemother,2007,51(10):3471-3484.
[7]Poirel L,Nordmann P.Carbapenem resistance in Acinetobacter baumannii:Mechanisms and epidemiology[J].Clio Microbiol Infect,2006,12(9):826-836.
[8]Vila J,MartíS,Sánchez-Céspedes J.Porins,efflux pumps and multidrug resistance in Acinetobacter baumannii[J].Antimicrob Chemother,2007,59(6):1210-1215.
[9]TONG Weihang,WANG Rui.Acinetobacter species carbapenem-resistance mechanisms and drug selection[J].Natl Med J China,2006,86(35):2516-2519.(in Chinese)童卫杭,王睿.不动杆菌属细菌对碳青霉烯类抗生素耐药机制及用药选择[J].中华医学杂志,2006,86(35):2516-2519.
[10]LING Baodong.Multidrug resistance in Acinetobacter baumannii:mechanisms and anti-infective therapy[J].Chin J Antibiot,2010,35(4):241-254.(in Chinese)凌保东.鲍曼不动杆菌抗生素多重耐药性:耐药机制与感染治疗对策[J].中国抗生素杂志,2010,35(4):241-254.
[11]Higgins PG,Dammhayn C,Hackel M,et al.Global spread of carbapenem-resistant Acinetobacter baumannii[J].J Antimicrob Chemother,2010,65(2):233-238.
[12]Akbari M,Niakan M,Taherikalani M,et al.Rapid identification of Iranian Acinetobacter baumannii strains by single PCR assay using BLA oxa-51-like carbapenemase and evaluation of the antimicrobial resistance profiles of the isolates[J].Acta Microbiol Immunol Hung,2010,57(2):87-94.
[13]LIN Jiafu,LIU Yongfang,ZENG Yue,et al.Detection of carbapenemases genes in carbapenem-resistant Acinetobacter baumannii[J].Chin J Nosocomiol,2015,25(17):3848-3880.(in Chinese)林家福,刘永芳,曾跃,等.耐碳青霉烯类鲍氏不动杆菌碳青霉烯酶基因检测研究[J].中华医院感染学杂志,2015,25(17):3848-3880.
[14]FAN Xuecai,WANG Xiaohong,ZHANG Jisheng,et al.Detection of carbapenem gene in Acinetobacter baumannii from Eastern Heilongjiang province[J].Chin J Microecol,2015,27(6):651-657.(in Chinese)范学财,王晓红,张吉生,等.黑龙江省东部地区鲍曼不动杆菌碳青霉烯酶基因检测[J].中国微生态学杂志,2015,27(6):651-657.