福建省3座水库库心沉积物聚磷菌的群落特征
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摘要
聚磷菌在水库沉积物磷的代谢循环中起着重要作用.采用T-RFLP技术和克隆测序,以多聚磷酸盐激酶基因(ppk1)作为标记基因,研究对比了福建省3座富营养化水库(九龙江西陂水库、平潭三十六脚湖水库和三明东牙溪水库)库心沉积物中聚磷菌的多样性及群落结构.结果表明:3座水库库心沉积物聚磷菌的多样性存在一定差异,但不显著,以三十六脚湖库心样品中聚磷菌多样性最高(Shannon指数H=2.89±0.03,Simpson指数D=0.06±0.01).克隆测序和比对结果分析表明,三十六脚湖水库沉积物样品中聚磷菌种类最多,结构最复杂,这与T-RFLP的结果一致.3座水库库心沉积物样品中聚磷菌优势菌属差异较为明显,但均以变形菌门、放线菌门和酸杆菌门为主,这3个门在3座水库库心沉积物样品中所占比重分别达74.5%、85.0%和75.0%.其中,变形菌门中的厌氧粘细菌属、酸杆菌门中的Solibacter属在3座水库库心沉积物样品中均为优势菌属.沉积物中Fe/Al-P与聚磷菌多样性指数的相关性最显著.Fe/Al-P对聚磷菌群落结构影响最为显著,3个水库共有优势菌属厌氧粘细菌与各形态磷呈正相关,且与OP、Ca-P等难溶解性磷呈显著相关,Solibacter与各形态磷均呈负相关.表明聚磷菌与富营养化水库沉积物磷代谢循环密切相关.
Polyphosphate accummulating organisms(PAOs) play an important role in the phosphorus metabolic cycling in the sediment of reservoir. We assessed the diversity and community structure of PAOs in the sediments by T-RFLP and clone sequencing which targeted ppk1 gene at the hearts of three reservoirs(Jiulongjiangxipi reservoir, Sanshiliujiao lake reservoir and Dongyaxi reservoir) in Fujian Province. The results showed that the diversity of PAOs varied among different reservoirs, though not statistically significant. The diversity of PAOs in the Sanshiliujiao lake reservoir was highest(Shannon index H=2.89±0.03, Simpson index D=0.06±0.01). The community structure of the PAOs in the Sanshiliujiao lake reservoir was most complicated, consistent with the results of the T-RFLP. The differences of dominant PAOs genera in three reservoirs were distinct, mainly concentrated in the Proteobacteria, Actinobacteria and Acidobacteria. The percentage of those three phylum accounted for 74.5%, 85.0% and 75.0%, respectively, of the total PAOs. The dominant groups in each reservoir sediment were Anaeromyxobacter and Solibacter. Various forms of phosphorus had certain influence on the diversity of PAOs. There were significantly correlation between Fe/Al-P and PAOs diversity and community structure. The dominant genus in the three reservoirs, Anaeromyxobacter, was positively correlated with all forms of phosphorus and significantly correlated with insoluble phosphorus such as OP and Ca-P, while Solibacter was negatively correlated with all forms of phosphorus. The results suggested that PAOs had important impacts on the phosphorus cycle of sediment in eutrophicatied reservoirs.
Polyphosphate accummulating organisms(PAOs) play an important role in the phosphorus metabolic cycling in the sediment of reservoir. We assessed the diversity and community structure of PAOs in the sediments by T-RFLP and clone sequencing which targeted ppk1 gene at the hearts of three reservoirs(Jiulongjiangxipi reservoir, Sanshiliujiao lake reservoir and Dongyaxi reservoir) in Fujian Province. The results showed that the diversity of PAOs varied among different reservoirs, though not statistically significant. The diversity of PAOs in the Sanshiliujiao lake reservoir was highest(Shannon index H=2.89±0.03, Simpson index D=0.06±0.01). The community structure of the PAOs in the Sanshiliujiao lake reservoir was most complicated, consistent with the results of the T-RFLP. The differences of dominant PAOs genera in three reservoirs were distinct, mainly concentrated in the Proteobacteria, Actinobacteria and Acidobacteria. The percentage of those three phylum accounted for 74.5%, 85.0% and 75.0%, respectively, of the total PAOs. The dominant groups in each reservoir sediment were Anaeromyxobacter and Solibacter. Various forms of phosphorus had certain influence on the diversity of PAOs. There were significantly correlation between Fe/Al-P and PAOs diversity and community structure. The dominant genus in the three reservoirs, Anaeromyxobacter, was positively correlated with all forms of phosphorus and significantly correlated with insoluble phosphorus such as OP and Ca-P, while Solibacter was negatively correlated with all forms of phosphorus. The results suggested that PAOs had important impacts on the phosphorus cycle of sediment in eutrophicatied reservoirs.
引文
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[15] Zhu Y-X (朱艳霞).Research on Metagenomie DNA and Biodiversity of Phosphorus-accumulating Organisms in the Sediments from Water Inlet of Taihu Lake.Master Thesis.Suzhou:Suzhou University of Science and Technology,2012 (in Chinese)
[16] Li Y,Cao WZ,Su CX,et al.Nutrient sources and composition of recent algal blooms and eutrophication in the northern Jiulong River,Southeast China.Marine Pollution Bulletin,2011,63:249-254
[17] Lin J (林佳),Zhong Y-P (钟燕平),Su Y-P (苏玉萍),et al.Research on the carbon,nitrogen,phosphrous and microalgae of sediments in Xipi Reservoir of Jiulong River.Journal of Fujian Teachers University (Natural Science) (福建师范大学学报:自然科学版),2015,31(5):63-69 (in Chinese)
[18] Weng C-Y (翁彩云).Analyses on water environment problems and engineering treating counter measure of surrounding in Sanshiliujiao Lake in Pingtan County.Energy and Environment (能源与环境),2009(3):11-13 (in Chinese)
[19] Chen Y-M (陈燕明).Analysis of the causes of water eutrophication and countermeasures in Dongyaxi reservoir.Straits Science (海峡科学),2016(6):44-46 (in Chinese)
[20] Ruban V,López-S昣nchez JF,Pardo P,et al.Harmonized protocol and certified reference material for the determination of extractable contents of phosphorus in freshwater sediments:A synthesis of recent works.Fresenius Journal of Analytical Chemistry,2001,370:224-228
[21] Ludwig F,Mehlig L,Eschenhagen M,et al.Development of new primer systems for the detection of the polyphosphate kinase gene in activated sludge.Water Science and Technology,2009,30:213-218
[22] Yin C-M (尹承苗),Wang G-S (王功帅),Li Y-Y (李园园),et al.Assessment of fungal diversity in apple replanted orchard soils by T-RFLP analysis.Acta Ecologica Sinica (生态学报),2014,34(4):837-846 (in Chinese)
[23] Chen Z-H (陈志豪),Liang X (梁雪),Li Y-C (李永春),et al.Effects of different fertilization regimes on soil fungal communities under Phyllostachys violascens stand.Chinese Journal of Applied Ecology (应用生态学报),2017,28(4):1168-1176 (in Chinese)
[24] Hu Y (胡颖),Wang S-H (王世和),Jiang H-L (江和龙),et al.Effect of different carbon resources on the enrichment of polyphosphate accumulating organisms in sediments from Taihu Lake.Safety and Environmental Engineering (安全与环境工程),2011,18(6):49-52 (in Chinese)
[25] Qiu C-S (邱春生),Nie H-L (聂海伦),Sun L-P (孙力平),et al.Metabolic properties of phosphorus-accumulating organisms at different carbon sources.Chinese Journal of Environmental Engineering (环境工程学报),2014,8(6):2191-2197 (in Chinese)
[26] Sun X (孙雪),Zhu W-J (朱为静),Wang L (王亮),et al.Review on the main microorganisms and their metabolic mechanisms in enhanced biological phos-phorus removal (EBPR) systems.Chinese Journal of Applied Ecology (应用生态学报),2014,25(3):892-902 (in Chinese)
[27] Wang Y-D (王亚东),Wang S-P (王少坡),Zheng S-S (郑莎莎),et al.Poly-P accumulating microorga-nisms and identifying methods for biological phosphorus removal system.Environmental Engineering(环境工程),2015,33(2):21-26 (in Chinese)
[28] Wu S-Y (伍思宇),Zhou Z-R (周志如),You Q (尤青).Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan,China.Chinese Journal of Applied & Environmental Biology (应用与环境生物学报),2016,22(3):397-401 (in Chinese)
[29] Liu H-L (刘鸿亮),Jin X-C (金相灿),Jing Y-F (荆一凤).Environmental dredging technology of lake sediment.Engineering Sciences (中国工程科学),1999,1(1):81-84 (in Chinese)
[30] He S,Gall DL,McMahon KD.Candidatus Accumulibacter population structure in enhanced biological phosphorus removal sludges as revealed by polyphosphate kinase genes.Applied and Environmental Microbiology,2007,73:5865-5874
[31] Kunin V,He S,Warnecke F,et al.A bacterial meta-population adapts locall to phase predation despite global dispersal.Genome Research,2008,18:293-297
[32] Hideyuki T,Yuji S,Satoshi H.Comparative analysis of bacterial diversity in fresh water sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques.Applied and Environmental Microbio-logy,2005,71:2162-2169
[33] Schwarz JIK,Eckert W,Conrad R.Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel).Systematic and Applied Micro-biology,2007,30:239-254
[2] Chen N (陈楠).Relationship between Microbial Community Structure and Nutrient Cycles and Algal Bloom in Taihu Lake.PhD Thesis.Beijing:China Agricultural University,2015 (in Chinese)
[3] Hu Y (胡颖),Jiang H-L (江和龙),He C-Q (何超群).Characteristic of physics and chemistry of grass/algae type zone sediments in Lake Taihu and effect on polyphosphate accumulation.Ecology and Environmental Sciences (生态环境学报),2012,21(11):1859-1864 (in Chinese)
[4] Dongye M-X (东野脉兴),Fan Z-Q (樊竹青),Zhang Z (张灼),et al.Experimental reseaches on phosphorus decomposing and concentrating of microorganism in Dianchi Lake:An example of modern phosphorus deposition and microorganism mineralization.Journal of Jilin University (Earth Science) (吉林大学学报:地球科学版),2003,33(3):282-289 (in Chinese)
[5] Xia X-H (夏学惠),Dongye M-X (东野脉兴),Zhou J-M (周建民),et al.Geochemistry and influence to environment of phosphorus in modern sediment in Dianchi Lake.Acta Sedimentologica Sinica (沉积学报),2002,20(3):416-420 (in Chinese)
[6] Weissbrodt DG,Holliger C,Morgenroth E.Modeling hydraulic transport and anaerobic uptake by PAOs and GAOs during wastewater feeding in EBPR granular sludge reactors.Biotechnology & Bioengineering,2017,114:1688-1702
[7] Chan C,Guisasola A,Baeza JA.Enhanced biological phosphorus removal at low sludge retention time in view of its integration in A-stage systems.Water Research,2017,118:217-226
[8] Wisniewski K,Kowalski M,Makinia J.Modeling nitrous oxide production by a denitrifying-enhanced biologically phosphorus removing (EBPR) activated sludge in the presence of different carbon sources and electron acceptors.Water Research,2018,142:55-64
[9] García Martín H,Ivanova N,Kunin V,et al.Meta-genomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities.Nature Biotechnology,2006,24:1263-1269
[10] Hupfer M,Gloess S,Grossart HP.Polyphosphate-accumulating microorganisms in aquatic sediments.Aquatic Microbial Ecology,2007,47:299-311
[11] Gonzalez-Gil G,Holliger C.Dynamics of microbial community structure of and enhanced biological phosphorus removal by aerobic granules cultivated on propionate or acetate.Applied and Environmental Microbiology,2011,77:8041-8051
[12] Qiao Z-H (乔子涵),Qi R (齐冉),Wang Z (王振),et al.Effects of carbon supplementary modes on phosphorus bioaccumulation/phosphorus harvesting process in a tidal flow constructed wetland.Chinese Journal of Applied Ecology (应用生态学报),2018,29(8):2753-2762 (in Chinese)
[13] Zhang L-M (张丽敏),Zeng W (曾薇),Wang A-Q (王安其),et al.Community structures and quantitative analyses of Candidatus accumulibacter in municipal wastewater treatment plants.Acta Scientiae Circumstantiae (环境科学学报),2016,36(4):1226-1235 (in Chinese)
[14] Skennerton CT,Barr JJ,Slater FR,et al.Expanding our view of genomic diversity in Candidatus Accumulibacter clades.Environmental Microbiology,2015,17:1574-1585
[15] Zhu Y-X (朱艳霞).Research on Metagenomie DNA and Biodiversity of Phosphorus-accumulating Organisms in the Sediments from Water Inlet of Taihu Lake.Master Thesis.Suzhou:Suzhou University of Science and Technology,2012 (in Chinese)
[16] Li Y,Cao WZ,Su CX,et al.Nutrient sources and composition of recent algal blooms and eutrophication in the northern Jiulong River,Southeast China.Marine Pollution Bulletin,2011,63:249-254
[17] Lin J (林佳),Zhong Y-P (钟燕平),Su Y-P (苏玉萍),et al.Research on the carbon,nitrogen,phosphrous and microalgae of sediments in Xipi Reservoir of Jiulong River.Journal of Fujian Teachers University (Natural Science) (福建师范大学学报:自然科学版),2015,31(5):63-69 (in Chinese)
[18] Weng C-Y (翁彩云).Analyses on water environment problems and engineering treating counter measure of surrounding in Sanshiliujiao Lake in Pingtan County.Energy and Environment (能源与环境),2009(3):11-13 (in Chinese)
[19] Chen Y-M (陈燕明).Analysis of the causes of water eutrophication and countermeasures in Dongyaxi reservoir.Straits Science (海峡科学),2016(6):44-46 (in Chinese)
[20] Ruban V,López-S昣nchez JF,Pardo P,et al.Harmonized protocol and certified reference material for the determination of extractable contents of phosphorus in freshwater sediments:A synthesis of recent works.Fresenius Journal of Analytical Chemistry,2001,370:224-228
[21] Ludwig F,Mehlig L,Eschenhagen M,et al.Development of new primer systems for the detection of the polyphosphate kinase gene in activated sludge.Water Science and Technology,2009,30:213-218
[22] Yin C-M (尹承苗),Wang G-S (王功帅),Li Y-Y (李园园),et al.Assessment of fungal diversity in apple replanted orchard soils by T-RFLP analysis.Acta Ecologica Sinica (生态学报),2014,34(4):837-846 (in Chinese)
[23] Chen Z-H (陈志豪),Liang X (梁雪),Li Y-C (李永春),et al.Effects of different fertilization regimes on soil fungal communities under Phyllostachys violascens stand.Chinese Journal of Applied Ecology (应用生态学报),2017,28(4):1168-1176 (in Chinese)
[24] Hu Y (胡颖),Wang S-H (王世和),Jiang H-L (江和龙),et al.Effect of different carbon resources on the enrichment of polyphosphate accumulating organisms in sediments from Taihu Lake.Safety and Environmental Engineering (安全与环境工程),2011,18(6):49-52 (in Chinese)
[25] Qiu C-S (邱春生),Nie H-L (聂海伦),Sun L-P (孙力平),et al.Metabolic properties of phosphorus-accumulating organisms at different carbon sources.Chinese Journal of Environmental Engineering (环境工程学报),2014,8(6):2191-2197 (in Chinese)
[26] Sun X (孙雪),Zhu W-J (朱为静),Wang L (王亮),et al.Review on the main microorganisms and their metabolic mechanisms in enhanced biological phos-phorus removal (EBPR) systems.Chinese Journal of Applied Ecology (应用生态学报),2014,25(3):892-902 (in Chinese)
[27] Wang Y-D (王亚东),Wang S-P (王少坡),Zheng S-S (郑莎莎),et al.Poly-P accumulating microorga-nisms and identifying methods for biological phosphorus removal system.Environmental Engineering(环境工程),2015,33(2):21-26 (in Chinese)
[28] Wu S-Y (伍思宇),Zhou Z-R (周志如),You Q (尤青).Screening and characterization of soil phosphate accumulating bacteria from Dongzhaigang mangrove wetlands in Hainan,China.Chinese Journal of Applied & Environmental Biology (应用与环境生物学报),2016,22(3):397-401 (in Chinese)
[29] Liu H-L (刘鸿亮),Jin X-C (金相灿),Jing Y-F (荆一凤).Environmental dredging technology of lake sediment.Engineering Sciences (中国工程科学),1999,1(1):81-84 (in Chinese)
[30] He S,Gall DL,McMahon KD.Candidatus Accumulibacter population structure in enhanced biological phosphorus removal sludges as revealed by polyphosphate kinase genes.Applied and Environmental Microbiology,2007,73:5865-5874
[31] Kunin V,He S,Warnecke F,et al.A bacterial meta-population adapts locall to phase predation despite global dispersal.Genome Research,2008,18:293-297
[32] Hideyuki T,Yuji S,Satoshi H.Comparative analysis of bacterial diversity in fresh water sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques.Applied and Environmental Microbio-logy,2005,71:2162-2169
[33] Schwarz JIK,Eckert W,Conrad R.Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel).Systematic and Applied Micro-biology,2007,30:239-254