基于当归/白芍提取物调节原发性痛经大鼠子宫供血的机制研究
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摘要
目的探讨当归/白芍提取物调节原发性痛经大鼠子宫供血的作用机制。方法雌性未孕Wistar大鼠随机分为空白组、模型组、治疗组、白芍组、当归组,采用缩宫素建立原发性痛经大鼠模型,用ELISA法检测各组大鼠血清中丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide dismutase,SOD)、精氨酸加压素(Arginine vasopressin,AVP)、缩宫素(Oxytocin,OT)的浓度及大鼠子宫组织中前列腺素F2α(Prostaglandin F2α,PGF2α)、前列腺素E2(Prostaglandin E2,PGE2)、一氧化氮(Nitric oxide,NO)、内皮素(Endothelin,ET)的含量。结果与空白组比较,模型组大鼠血清中MDA、AVP、OT浓度明显升高,SOD浓度降低,子宫组织中PGF2α、PGE2含量均明显升高,NO含量显著下降,ET的含量显著上升(P<0.05)。与模型组比较,治疗组大鼠血清中MDA、AVP、OT浓度明显降低,SOD浓度显著升高,大鼠子宫组织中PGF2α、PGE2含量明显降低,NO含量增加,ET含量明显下降,差异具有统计学意义(P<0.05)。结论当归/白芍提取物能通过抑制子宫收缩,调节血管舒缩物质,改善子宫供血,从而达到对原发性痛经的治疗作用。
Objective To determine the mechanism of Angelica sinensis(Paeonia lactiflora Pall.) extract in regulating uterine blood supply in rats with primary dysmenorrhea. Methods Female non-pregnant Wistar rats were randomly divided into a control group, a model group, a treatment group, a Radix Paeoniae Alba group, and an Angelica group. Oxytocin was used to establish the primary dysmenorrhea rat model. ELISA was used to detect the serum MDA, SOD, AVP, and PGF2α, and uterine tissue concentration of OT, PGE2, NO and ET. Results Compared with those of the blank group, in the the model group rats the serum MDA, AVP, and OT concentration increased significantly, the concentration of SOD reduced, while PGF2α and PGE2 in uterine tissues was significantly increased. NO content was decreased significantly, and the content of ET was increased(P < 0.05). Compared with those of the model group, the treatment group serum MDA, AVP, and OT concentrations were significantly decreased, SOD concentration increased significantly, PGF2α and PGE2 content in the rat uterus decreased, NO content increased and ET content decreased; with significant difference(P< 0.05). Conclusion Radix Angelica Sinensis and Radix Paeoniae Alba extract can inhibit uterine contraction, regulate vasomotor substances, improve uterine blood supply, which have therapeutic effect on primary dysmenorrhea.
Objective To determine the mechanism of Angelica sinensis(Paeonia lactiflora Pall.) extract in regulating uterine blood supply in rats with primary dysmenorrhea. Methods Female non-pregnant Wistar rats were randomly divided into a control group, a model group, a treatment group, a Radix Paeoniae Alba group, and an Angelica group. Oxytocin was used to establish the primary dysmenorrhea rat model. ELISA was used to detect the serum MDA, SOD, AVP, and PGF2α, and uterine tissue concentration of OT, PGE2, NO and ET. Results Compared with those of the blank group, in the the model group rats the serum MDA, AVP, and OT concentration increased significantly, the concentration of SOD reduced, while PGF2α and PGE2 in uterine tissues was significantly increased. NO content was decreased significantly, and the content of ET was increased(P < 0.05). Compared with those of the model group, the treatment group serum MDA, AVP, and OT concentrations were significantly decreased, SOD concentration increased significantly, PGF2α and PGE2 content in the rat uterus decreased, NO content increased and ET content decreased; with significant difference(P< 0.05). Conclusion Radix Angelica Sinensis and Radix Paeoniae Alba extract can inhibit uterine contraction, regulate vasomotor substances, improve uterine blood supply, which have therapeutic effect on primary dysmenorrhea.
引文
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[19]曾玉燕,李坤寅,关永格.加味芍药甘草汤及含药血清对子宫腺肌病E2、ER及芳香化酶P450的影响[J].北京中医药大学学报,2017,40(9):722-728.
[20]范恺磊,蔡皓,刘晓,等.香附与艾叶对治疗原发性痛经作用机制的研究进展[J].中药新药与临床药理,2017,28(1):139-143.
[21]马跃,杨燕云,张振秋,等.芍药甘草汤不同极性部位对痛经模型大鼠MDA、SOD、GSH-PX、ROS、NO、PGE2、PGF2α影响[J].辽宁中医药大学学报,2017,19(3):32-34.
[22]周启微,郭美仙,杨佳晶,等.当归对缩宫素所致小鼠原发性痛经的机制研究[J].大理大学学报,2017,2(4):24-27.
[23]宋娟利.桂枝茯苓胶囊对子宫内膜异位症不孕患者外周血TNF-α、SOD水平的影响[J].现代中西医结合杂志,2016,25(20):2262-2263.
[2]夏宛廷,梁潇元,耿静然,等.《金匮要略》当归芍药散治疗痛经之浅析[J].中国中医急症,2017,26(3):451-453.
[3]吴梓春,刘肖林.中药当归有效成分分离及含量测定[J].北方药学,2015,12(8):15-16.
[4]刘辉,赵乾,薛胜霞,等.高效液相色谱法测定逍遥丸中芍药苷和甘草酸的含量[J].海峡药学,2018,20(2):56-58.
[5]杨必浩,周应硕,姚翠蓉.高效液相色谱法同时测定八珍丸中芍药苷、阿魏酸的含量[J].系统医学,2017,2(12):120-123+127.
[6]嵇波,张露芬,朱江,等.痛经模型建立和评价方法的思考[J].中国药理学通报,2008,24(6):711-714.
[7]柯琴,阮诗兰,李大剑.原发性痛经的动物实验研究进展[J].临床医学,2009,29(8):101-102.
[8]聂超凤,严金婵,廖旭日,等.少腹逐瘀汤合四逆汤对寒凝血瘀型原发性痛经患者PGE2、PGF2α、β-EP含量的影响[J].现代中西医结合杂志,2017,26(14):1515-1517+1557.
[9]张福云,王文华,陈琳,等.暖宫七味散对原发性痛经寒凝血滞证患者血清PGF2α、PGE2水平及子宫微循环的影响[J].中国现代医生,2017,55(23):10-12+16.
[10]孙秀凤.痛经舒汤加减联合董氏奇穴常规穴针刺治疗痛经对症状评分及预后影响[J].河北医学,2017,23(5):848-851.
[11]寇小平,邵莹,卢占斌.前列腺素PGF2α受体与原发性痛经关系[J].现代仪器与医疗,2016,22(2):110-112.
[12]李英,孙兴云.布洛芬缓释胶囊联合经痛康颗粒对原发性痛经患者炎症因子、β-EP、PGE2及PGF2α的影响[J].中国妇幼保健,2015,30(17):2854-2856.
[13]范春香,唐苾芯,高颖,等.经痛宁方对气滞血瘀型原发性痛经患者血清PGF2α、NO、OT的影响[J].新中医,2015,47(1):159-161.
[14]何瑶,王丽娟,刘婷婷,等.基于代谢组学技术分析四物汤治疗原发性痛经证的作用机制[J].中国实验方剂学杂志,2017,23(12):82-89.
[15]孙玉阳,纪宏宇,陈博,等.原发性痛经的发病机制及中医药治疗的研究进展[J].中国药师,2017,20(1):144-147.
[16]曹秋实,李德顺,余青,等.当归-川芎对原发性痛经小鼠E2、AVP、OT表达的影响[J].江西中医药,2016,47(11):36-38.
[17]黄苗苗,何晔,李绪清,等.缩宫素受体在子宫内膜异位症子宫结合带中的表达[J].中华生殖与避孕杂志,2017,37(3):203-207.
[18]王艳英.原发性痛经发病机制及治疗的研究进展[J].中华中医药杂志,2015,30(7):2447-2449.
[19]曾玉燕,李坤寅,关永格.加味芍药甘草汤及含药血清对子宫腺肌病E2、ER及芳香化酶P450的影响[J].北京中医药大学学报,2017,40(9):722-728.
[20]范恺磊,蔡皓,刘晓,等.香附与艾叶对治疗原发性痛经作用机制的研究进展[J].中药新药与临床药理,2017,28(1):139-143.
[21]马跃,杨燕云,张振秋,等.芍药甘草汤不同极性部位对痛经模型大鼠MDA、SOD、GSH-PX、ROS、NO、PGE2、PGF2α影响[J].辽宁中医药大学学报,2017,19(3):32-34.
[22]周启微,郭美仙,杨佳晶,等.当归对缩宫素所致小鼠原发性痛经的机制研究[J].大理大学学报,2017,2(4):24-27.
[23]宋娟利.桂枝茯苓胶囊对子宫内膜异位症不孕患者外周血TNF-α、SOD水平的影响[J].现代中西医结合杂志,2016,25(20):2262-2263.