GC-MS结合AMDIS和保留指数法分析白术芍药散及其组方饮片中挥发油的组成
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摘要
目的研究白术芍药散各组方饮片组成复方前后挥发油的组成成分变化。方法通过水蒸气蒸馏法提取白术芍药散及其组方饮片(炒白术、炒白芍、炒陈皮、防风)中的挥发油,经GC-MS测定得到总离子流图谱,利用自动质谱退卷积定性系统(AMDIS)和保留指数(RI)对检测结果进行定性分析,应用峰面积归一法计算各化合物的相对含量。结果研究结果表明白术芍药散的挥发油主要来源于炒白术、炒陈皮和防风,组方饮片经配伍后,其挥发油的成分与比例发生明显变化。结论该研究可为白术芍药散组方的物质基础和配伍机理的挖掘奠定基础。
OBJECTIVE The experiment was designed to investigate the changes of the chemical constituents from the essential oils of Baizhu Shaoyao San and the medicinal herbs contained in Baizhu Shaoyao San.METHODS The essential oils from Baizhu Shaoyao San and the medicinal herbs(stir-baked Atractylodis Macrocephalae Rhizoma,stir-baked Paeoniae Radix Alba,stir-baked Citri Reticulatae Pericarpium,Saposhnikoviae Radix)contained in Baizhu Shaoyao San were extracted by steam distillation method.GC-MS combined with automated mass spectral deconrolution and identification system(AMDIS)and retention index were employed to identify the chemical components of the essential oils and the relative amounts of these chemical constituents were calculated by peak area normalization method.RESULTS The results showed that the essential oils of Baizhu Shaoyao San were mainly derived from stir-baked Atractylodis Macrocephalae Rhizoma,stir-baked Citri Reticulatae Pericarpium,and Saposhnikoviae Radix.The quantity and quality of the volatile constituents from the medicinal herbs contained in Baizhu Shaoyao San experienced apparent changes after their compatibility.CONCLUSION Our investigation can make a good foundation for the material basis and the compatibility mechanism of Baizhu Shaoyao San.
OBJECTIVE The experiment was designed to investigate the changes of the chemical constituents from the essential oils of Baizhu Shaoyao San and the medicinal herbs contained in Baizhu Shaoyao San.METHODS The essential oils from Baizhu Shaoyao San and the medicinal herbs(stir-baked Atractylodis Macrocephalae Rhizoma,stir-baked Paeoniae Radix Alba,stir-baked Citri Reticulatae Pericarpium,Saposhnikoviae Radix)contained in Baizhu Shaoyao San were extracted by steam distillation method.GC-MS combined with automated mass spectral deconrolution and identification system(AMDIS)and retention index were employed to identify the chemical components of the essential oils and the relative amounts of these chemical constituents were calculated by peak area normalization method.RESULTS The results showed that the essential oils of Baizhu Shaoyao San were mainly derived from stir-baked Atractylodis Macrocephalae Rhizoma,stir-baked Citri Reticulatae Pericarpium,and Saposhnikoviae Radix.The quantity and quality of the volatile constituents from the medicinal herbs contained in Baizhu Shaoyao San experienced apparent changes after their compatibility.CONCLUSION Our investigation can make a good foundation for the material basis and the compatibility mechanism of Baizhu Shaoyao San.
引文
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[4]高蓓.广陈皮黄酮类化合物和挥发油成分及其活性研究[D].武汉:华中农业大学,2011.
[5]瞿白露,吴泓毅,任凤莲,等.GC/MS分析白芍挥发油的成分[J].食品科技,2009,34(3):92-95.
[6]崔景朝,赵自明.中药配方颗粒研究进展(Ⅱ)——中药单煎与合煎对比研究概况[J].中国实验方剂学杂志,2011,17(4):240-245.
[7]MARTINS C,DORAN C,LAIRES A,et al.Genotoxic and apoptotic activities of the food flavourings myristicin and eugenol in AA8and XRCC1deficient EM9cells[J].Food Chem Toxicol,2011,49(2):385-392.
[8]王梅兰,林建交,陈雅容.柠檬烯对小白鼠中枢神经系统的影响[J].海峡药学,2005,17(4):30-32.
[9]王东风,梁惠,王文成.D-柠檬烯对大鼠酒精性肝损伤脂质代谢紊乱的影响[J].食品科学,2015,36(5):163-167.
[10]PICCINELLI AC,SANTOS JA,KONKIEWITZ EC,et al.Antihyperalgesic and antidepressive actions of(R)-(+)-limonene,α-phellandrene,and essential oil fromSchinus terebinthifolius fruits in a neuropathic pain model[J].Nutr Neurosci,2015,18(5):217-224.
[11]HWANG JM,TSENG TH,HSIEH YS,et al.Inhibitory effect of atractylon on tert-butyl hydroperoxide induced DNA damage and hepatic toxicity in rat hepatocytes[J].Arch Toxicol,1996,70(10):640-644.