基于UPLC-Q-TOF/MS技术的玉竹化学成分分析
摘要
目的应用超高效液相色谱-飞行时间质谱联用技术(UPLC-Q-TOF/MS)对玉竹甲醇提取物进行了化学成分分析。方法色谱采用waters C18色谱柱(2.1×100 mm,1.7μm),0.1%甲酸水-0.1%甲酸乙腈梯度洗脱,质谱负离子模式扫描。结果共表征或鉴定30个化合物,包括15个高异黄酮类成分,8个酰胺生物碱类成分、4个螺甾皂苷类成分及3个其他成分,其中8个化合物为玉竹质谱分析的首次报道。结论 UPLC-QTOF/MS技术能够提供丰富稳定的裂解碎片离子,为进一步研究玉竹体内药效物质基础提供科学基础数据。
引文
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[6]张沐新.玉竹甾体皂苷活性成分和分蘗葱头黄酮类活性成分及工艺研究.长春:吉林大学, 2007.
[2]李妙然,秦灵灵,魏颖,等.玉竹化学成分与药理作用研究进展.中华医药药学刊, 2015, 33(8):1939-1943.
[3]周劭宣,刘玥欣,黄晓巍,等.药食两用中药玉竹药理作用及其应用的研究进展.人参研究, 2017(3):53.
[4] Ye M, Guo D,Ye G, et al. Analysis of Homoisoflavonoids in Ophiopogon japonicus by HPLC-DAD-ESI-MSn. J Am Soc Mass Spectrom,2005, 16(2):234-243.
[5] Hu X, Zhao H, Shi S, et al. Sensitive characterization of polyphenolic antioxidants in Polygonatum odoratum by selective solid phase extraction and high performance liquid chromatography-diode array detector-quadrupole timeof-flight tandem mass spectrometry. J Pharm Biomed Anal, 2015, 112:15-22.
[6]张沐新.玉竹甾体皂苷活性成分和分蘗葱头黄酮类活性成分及工艺研究.长春:吉林大学, 2007.