摘要
目的探讨颅脑创伤后期(第7天)大鼠脑组织代谢型谷氨酸受体5(m Glu R5)表达变化,以及牛磺酸治疗作用。方法液压脑损伤打击仪制备液压打击颅脑创伤大鼠模型,采用随机数字表法将30只无特定病原体级Sprague-Dawley大鼠随机分为对照组、颅脑创伤组和牛磺酸治疗组(各10只),干湿重法检测大鼠脑组织含水量,实时荧光定量聚合酶链反应和Western blotting法检测水通道蛋白4(AQP4)和m Glu R5 m RNA和蛋白表达变化。结果与对照组相比,颅脑创伤组大鼠脑组织含水量(t=4.893,P=0.002)、AQP4 m RNA(t=6.523,P=0.000)和蛋白(t=4.366,P=0.008)表达水平升高,m Glu R5m RNA(t=5.776,P=0.001)和蛋白(t=3.945,P=0.014)表达水平降低;经牛磺酸治疗后,大鼠脑组织含水量(t=2.151,P=0.140)、AQP4 m RNA(t=1.144,P=0.432)和蛋白(t=0.367,P=0.804)降至正常水平,m Glu R5 m RNA(t=1.824,P=0.216)和蛋白(t=1.185,P=0.414)升至正常水平。相关分析显示,脑组织含水量与m Glu R5 m RNA(r=-0.617,P=0.014)和蛋白(r=-0.665,P=0.007)呈负相关,与AQP4蛋白呈正相关(r=0.658,P=0.008)。结论牛磺酸可以升高颅脑创伤后期(第7天)大鼠脑组织m Glu R5表达水平,降低脑水肿和脑组织含水量,具有抑制性神经递质作用。
Objective To investigate the change of metabotropic glutamate receptor 5(m Glu R5)expression level in rats with late-stage(the 7th day) traumatic brain injury(TBI) and the role of taurine.Methods The left cerebral TBI rat models were made by using lateral fluid percussion method. A total of30 specific pathogen free(SPF) male Sprague-Dawley(SD) rats were randomly divided into 3 groups: shamoperation group(control group), TBI model group(TBI group) and taurine treatment group(taurine group).Wet and dry weight method was used to measure the brain water content. Real- time fluorescentquantitative polymerase chain reaction(PCR) and Western blotting were used to detect the change of m RNAand protein expression of aquaporin 4(AQP4) and m Glu R5 in each group. Results Compared withcontrol group, the brain water content(t = 4.893, P = 0.002), AQP4 m RNA(t = 6.523, P = 0.000) and protein(t = 4.366, P = 0.008) expression were upregulated, while m Glu R5 m RNA(t = 5.776, P = 0.001) and protein(t = 3.945, P = 0.014) expression were downregulated in TBI group. After taurine treatment, the brain watercontent(t = 2.151, P = 0.140), AQP4 m RNA(t = 1.144,P = 0.432) and protein(t = 0.367, P = 0.804)decreased to normal, while m Glu R5 m RNA(t = 1.824, P = 0.216) and protein(t = 1.185, P = 0.414) increasedto normal. Correlation analysis showed brain water content was negatively correlated with m Glu R5 m RNA(r =- 0.617, P = 0.014) and m Glu R5 protein(r =- 0.665, P = 0.007), while it was positively correlated withAQP4 protein(r = 0.658, P = 0.008). Conclusions Taurine can significantly increase the m Glu R5 expression level of brain issue in the late-stage(the 7th day) of TBI and decline brain edema and brainwater content. It may be a potential protective agent as an inhibitory neurotransmitter.
引文
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