光电化学生物传感器用于测定丁草胺
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摘要
制备了ITO/Gr/CH_3NH_3PbI_3/CS/GOx电极,将制得的电极浸入5.0mL的0.1mol·L~(-1) PBS(pH 7.0)中,加入丁草胺标准溶液,保持10min,然后将电极取出插入含有0.8mmol·L~(-1)葡萄糖的0.1mol·L~(-1) PBS(pH 7.0)中,测量光电流(I)。另做空白试验(操作同上,但不存在丁草胺),测量光电流(I_0)。由(I_0-I)/I_0×100计算丁草胺对光电流的抑制率。结果表明,抑制率与丁草胺的浓度在0.02～10.0nmol·L~(-1)内呈线性关系,检出限(3S/N)为0.005nmol·L~(-1)。方法用于测定蔬菜和水果空白加标样品中的丁草胺,测定值与气相色谱-质谱法的测定结果一致,测定值的相对标准偏差(n=6)在1.8%～4.7%之间,加标回收率在93.3%～102%之间。
The electrode of ITO/Gr/CH_3 NH_3 PbI_3/CS/GOx was prepared.The obtained electrode was immersed into 5.0 mL of 0.1 mol·L~(-1) PBS(pH 7.0),and butachlor solution was added.After keeping for 10 min,the electrode was taken out and immersed into 0.1 mol·L~(-1) PBS(pH 7.0)containing 0.8 mmol·L~(-1) gluocose,and the photoelectric current(I)was measured.A blank test was done in the same way as mentioned above but in absence of butachlor,and the photoelectric current(I_0)was measured.Inhibition rate of photoelectric current by butachlor was calculated from(I_0-I)/I_0×100.The results showed that inhibition rate was linear with the concentration of butachlor in the range of 0.02-10.0 nmol·L~(-1),and the detection limit(3 S/N)was 0.005 nmol·L~(-1).The method was applied to determination of butachlor in spiked blank samples of vegetables and fruits,giving measured values consistent with those obtained by gas chromatography-mass spectrometry,and RSDs(n=6)between 1.8% and 4.7%.Recovery obtained by standard addition method were in the range of 93.3%-102%.
The electrode of ITO/Gr/CH_3 NH_3 PbI_3/CS/GOx was prepared.The obtained electrode was immersed into 5.0 mL of 0.1 mol·L~(-1) PBS(pH 7.0),and butachlor solution was added.After keeping for 10 min,the electrode was taken out and immersed into 0.1 mol·L~(-1) PBS(pH 7.0)containing 0.8 mmol·L~(-1) gluocose,and the photoelectric current(I)was measured.A blank test was done in the same way as mentioned above but in absence of butachlor,and the photoelectric current(I_0)was measured.Inhibition rate of photoelectric current by butachlor was calculated from(I_0-I)/I_0×100.The results showed that inhibition rate was linear with the concentration of butachlor in the range of 0.02-10.0 nmol·L~(-1),and the detection limit(3 S/N)was 0.005 nmol·L~(-1).The method was applied to determination of butachlor in spiked blank samples of vegetables and fruits,giving measured values consistent with those obtained by gas chromatography-mass spectrometry,and RSDs(n=6)between 1.8% and 4.7%.Recovery obtained by standard addition method were in the range of 93.3%-102%.
引文
[1]姚斌,徐建民,张超兰.除草剂丁草胺的环境行为综述[J].生态环境,2003,12(1):66-70.
[2] NY/T 419-2014绿色食品稻米[S].
[3]姚杰,刘传德,周先学,等.基于气相色谱-氮磷检测法同时检测玉米中莠去津、乙草胺和丁草胺的残留量[J].中国农学通报,2018,34(9):127-130.
[4]赵锋,刘思宏,黄璐璐,等.气相色谱法检测丁草胺和乙氧氟草醚在甘蔗与土壤中的残留量[J].农药,2016,55(8):597-599.
[5]李新安,陈锡岭,赵华.高效液相色谱法同时测定棉花及土壤中丁草胺和异噁草酮的残留[J].浙江农业学报,2011,23(6):1172-1176.
[6] DEVADOSS A,SUDHAGAR P,TERASHIMA C,et al.Photoelectrochemical biosensors:New insights into promising photoelectrodes and signal amplification strategies[J].Journal of Photochemistry and Photobiology C:Photochemistry Reviews,2015,24:43-63.
[7] LIAO L,LIN Y C,BAO M Q,et al.High-speed graphene transistors with a self-aligned nanowire gate[J].Nature,2010,467(7313):305-308.
[8] GARAJ S,HUBBARD W,REINA A,et al.Graphene as a subnanometre trans-electrode membrane[J].Nature,2010,467(7312):190-193.
[9] XING G,MATHEWS N,SUN S,et al.Long-range balanced electron-and hole-transport lengths in organic-inorganic CH3NH3PbI3[J].Science,2013,342(6156):344-347.
[10] XU R,JIANG Y D,XIA L,et al.A sensitive photoelectrochemical biosensor for AFP detection based on ZnO inverse opal electrodes with signal amplification of CdS-QDs[J].Biosensors and Bioelectronics,2015,74:411-417.
[11] KISKU S K,SWAIN S K.Synthesis and characterization of Chitosan/Boron nitride composites[J].Journal of the American Ceramic Society,2012,95(9):2753-2757.
[12] LIU M Z,JOHNSTON M B,SNAITH H J.Efficient planar heterojunction perovskite solar cells by vapour deposition[J].Nature,2013,501(7467):395-398.
[13] JIN D Q,GONG A Q,ZHOU H.Visible-light-activated photoelectrochemical biosensor for the detection of the pesticide acetochlor in vegetables and fruit based on its inhibition of glucose oxidase[J].RSC Advances,2017,7(28):17489-17496.
[14] WANG Y H,LIU H Y,WANG P P,et al.Chemiluminescence excited photoelectrochemical competitive immunosensing lab-on-paper device using an integrated paper supercapacitor for signal amplication[J].Sensors and Actuators B:Chemical,2015,208:546-553.
[15] LIU J X,XU X M,TANG L,et al.Determination of trace mercury in compost extract by inhibition based glucose oxidase biosensor[J].Transactions of Nonferrous Metals Society of China,2009,19(1):235-240.
[2] NY/T 419-2014绿色食品稻米[S].
[3]姚杰,刘传德,周先学,等.基于气相色谱-氮磷检测法同时检测玉米中莠去津、乙草胺和丁草胺的残留量[J].中国农学通报,2018,34(9):127-130.
[4]赵锋,刘思宏,黄璐璐,等.气相色谱法检测丁草胺和乙氧氟草醚在甘蔗与土壤中的残留量[J].农药,2016,55(8):597-599.
[5]李新安,陈锡岭,赵华.高效液相色谱法同时测定棉花及土壤中丁草胺和异噁草酮的残留[J].浙江农业学报,2011,23(6):1172-1176.
[6] DEVADOSS A,SUDHAGAR P,TERASHIMA C,et al.Photoelectrochemical biosensors:New insights into promising photoelectrodes and signal amplification strategies[J].Journal of Photochemistry and Photobiology C:Photochemistry Reviews,2015,24:43-63.
[7] LIAO L,LIN Y C,BAO M Q,et al.High-speed graphene transistors with a self-aligned nanowire gate[J].Nature,2010,467(7313):305-308.
[8] GARAJ S,HUBBARD W,REINA A,et al.Graphene as a subnanometre trans-electrode membrane[J].Nature,2010,467(7312):190-193.
[9] XING G,MATHEWS N,SUN S,et al.Long-range balanced electron-and hole-transport lengths in organic-inorganic CH3NH3PbI3[J].Science,2013,342(6156):344-347.
[10] XU R,JIANG Y D,XIA L,et al.A sensitive photoelectrochemical biosensor for AFP detection based on ZnO inverse opal electrodes with signal amplification of CdS-QDs[J].Biosensors and Bioelectronics,2015,74:411-417.
[11] KISKU S K,SWAIN S K.Synthesis and characterization of Chitosan/Boron nitride composites[J].Journal of the American Ceramic Society,2012,95(9):2753-2757.
[12] LIU M Z,JOHNSTON M B,SNAITH H J.Efficient planar heterojunction perovskite solar cells by vapour deposition[J].Nature,2013,501(7467):395-398.
[13] JIN D Q,GONG A Q,ZHOU H.Visible-light-activated photoelectrochemical biosensor for the detection of the pesticide acetochlor in vegetables and fruit based on its inhibition of glucose oxidase[J].RSC Advances,2017,7(28):17489-17496.
[14] WANG Y H,LIU H Y,WANG P P,et al.Chemiluminescence excited photoelectrochemical competitive immunosensing lab-on-paper device using an integrated paper supercapacitor for signal amplication[J].Sensors and Actuators B:Chemical,2015,208:546-553.
[15] LIU J X,XU X M,TANG L,et al.Determination of trace mercury in compost extract by inhibition based glucose oxidase biosensor[J].Transactions of Nonferrous Metals Society of China,2009,19(1):235-240.