基于氧化锌-富勒烯/卵磷脂复合纳米膜界面的顺式茉莉酮的电化学分析研究
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摘要
顺式-茉莉酮是花挥发物的一种植物激素成分,是胁迫条件下茉莉酸的代谢物之一,具有生理活性,在植物防御中起重要作用.顺式-茉莉酮的快速、灵敏检测对于研究植物的生理作用具有重要意义.将氧化锌/富勒烯(Zn O/C60)复合纳米粒子分散在卵磷脂(PC)溶液中,滴涂在玻碳电极表面,制备了基于PC-Zn O/C60复合纳米修饰膜电极.采用伏安法比较了顺式-茉莉酮在不同修饰电极、不同酸度下的电化学行为.顺式茉莉酮的还原峰电流与浓度在1.0×10-51.0×10-3mol/L范围内具有良好的线性关系,检测限为9.2×10-6mol/L.该传感器对顺式-茉莉酮具有灵敏的电化学响应信号,可以实现顺式-茉莉酮的灵敏检测.
Cis-jasmone is plant hormone components of flower volatile,which is one kind of jasmonic acid metabolites on the stress conditions with physiological activity and plays an important role in plant defense. The rapid and sensitive detection of cis-jasmone is of great significance to study the physiological functions of plants. In this article,zinc oxide-fullerenes( Zn O/C60) composite nanoparticles were dispersed into lecithin( PC) solution by ultrasound in order to modify the surface of glassy carbon electrode to prepare an electrochemical sensor based on zinc oxide/fullerenes/lecithin composite nanometer membrane. The electrochemical behaviors of cis-jasmone on glassy carbon electrode modified by PC or PC-Zn O/C60 and under different acidity were discussed. Cis-jasmone could be determined selectively and sensitively in a linear range from 1. 0 × 10-5 mol/L to 1. 0 × 10-3 mol/L. The limit of detection was 9. 2 × 10-6 mol/L. The sensor has sensitive electrochemical voltammetric response to cis-jasmone and can be applied to the actual test.
Cis-jasmone is plant hormone components of flower volatile,which is one kind of jasmonic acid metabolites on the stress conditions with physiological activity and plays an important role in plant defense. The rapid and sensitive detection of cis-jasmone is of great significance to study the physiological functions of plants. In this article,zinc oxide-fullerenes( Zn O/C60) composite nanoparticles were dispersed into lecithin( PC) solution by ultrasound in order to modify the surface of glassy carbon electrode to prepare an electrochemical sensor based on zinc oxide/fullerenes/lecithin composite nanometer membrane. The electrochemical behaviors of cis-jasmone on glassy carbon electrode modified by PC or PC-Zn O/C60 and under different acidity were discussed. Cis-jasmone could be determined selectively and sensitively in a linear range from 1. 0 × 10-5 mol/L to 1. 0 × 10-3 mol/L. The limit of detection was 9. 2 × 10-6 mol/L. The sensor has sensitive electrochemical voltammetric response to cis-jasmone and can be applied to the actual test.
引文
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[2]Jin H,Zhu Z.Temporal and spatial view of jasmonate signaling[J].Trends Plant Sci,2017,22(6):451-454.
[3]Lehmann J,Atzorn R,Briickner C.Accumulation of jasmonate,abscisic acid,specific transcripts and proteins in osmotically stressed barley leaf segments[J].Planta,1995,167:156-162.
[4]Muhammad N,Subhani Q,Wang F,et al.Simultaneous determination of two plant growth regulators in ten food samples using ion chromatography combined with Qu ECh ERS extraction method(IC-Qu ECh ERS)and coupled with fluorescence detector[J].Food Chem,2017,241:308-316.
[5]Liu X,Yang Y,Lin W,et al.Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry[J].Chinese Sci Bull,2010,55(21):2231-2235.
[6]Zhang W,Du J,Su C,et al.Development ofβ-cyclodextrin-modified silica and polyporous polymer particles for solid-phase extraction of methyl jasmonate in aqueous and plant samples[J].Anal Lett,2013,46(6):900-911.
[7]Yu X,Ling X,Zou L,et al.Novel polymeric monolith materials with aβ-cyclodextrin-graphene composite for the highly selective extraction of methyl jasmonate[J].J Sep Sci,2017,40(7):1556-1563.
[8]Gan T,Hu C,Chen Z,et al.Fabrication and application of a novel plant hormone sensor for the determination of methyl jasmonate based on self-assembling of phosphotungstic acid-graphene oxide nanohybrid on graphite electrode[J].Sens,Actuators B:Chemical,2010,151(1):8-14.
[9]Gan T,Hu C,Chen Z,et al.Direct electrochemical determination of methyl jasmonate in wheat spikelet at a nanomontmorillonite film modified electrode by derivative square wave voltammetry[J].J Agric Food Chem,2010,58(16):8942-8947.
[10]Liao Y,Wang F,Chen Z.Electrochemical behavior and the determination of methyl jasmonate.Chinese Sci[J].Bull,2010,55(21):2225-2230.
[11]Azam A,Ahmed F,Arshi N,et al.Formation and characterization of Zn O nanopowder synthesized by sol-gel method[J].J Alloys Compd,2010,496(1/2):399-402.
[12]Mojica M,Alonso J A,Méndez F.Synthesis of fullerenes[J].J Phys Org Chem,2013,26(7):526-539.
[13]Zhi M,Xiang C,Li J,et al.Nanostructured carbon-metal oxide composite electrodes for supercapacitors:a review[J].Nanoscale,2013,5(1):72-88.
[14]Dang X,Hu C,Chen Z,et al.Electrochemical characteristics of cis-jasmone in acid media at multi-wall carbon nanotubeNafion composite film modified electrode and its analytical application[J].Electrochim Acta,2012,81:239-245.
[15]Willbert S,Ericsson L,Gordon M,et al.Quantification of jasmonic acid,methyl jasmonate and salicylic acid in plants by capillary liquid chromatography electrospray tendem mass spectrometry[J].Anal Biochem,1998,257:186-194.