外源物质对红松多酚合成和防御酶活性的影响
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摘要
研究3种外源物质(壳聚糖CTS、茉莉酸甲酯MeJA、硝酸镧La)对松多酚合成的诱导作用。以组织培养诱导生成的红松不定芽为试验材料,CTS、MeJA、La处理8d过程中对不定芽生长、松多酚积累量、多酚合成关键酶(苯丙氨酸转移酶PAL、肉桂酸4-羟化酶C4H)、防御酶(超氧化物歧化酶SOD、过氧化物酶POD、过氧化氢酶CAT)影响。结果表明,CTS、MeJA、La均能促进松多酚的合成,其诱导效果存在差异,以La诱导效果最显著。在试验条件浓度下,La不仅可以促进红松不定芽的生长,且能显著提高总酚和原花青素的积累量。经La处理的红松不定芽中的SOD、POD、CAT、PAL、C4H能够迅速响应,并且活性均呈现先升高后降低的趋势,各酶活峰值分别是对照组的1.79、1.51、1.33、3.98倍和3.61倍。CTS、MeJA、La 3种外源物质能激活红松防御反应,促进次生代谢产物松多酚的合成。
The objectives of this study were to investigate the induction of pine polyphenols biosynthesis by three exogenous substances including chitosan(CTS),methyl jasmonate(MeJA)and lanthanum nitrate(La).Adventitious bud of Pinus koraiensis was used as the material,the effects of three exogenous substances were examined on the growth,the pine polyphenols accumulation,the key biosynthesis enzymes and the defense enzymes.The results showed that CTS,MeJA and La had the abilities to facilitate the syntheses of pine polyphenol with different efficiencies,in which La presented the most significant effects.In the condition of examination concentration,La was able not only to facilitate a growth of adventitious bud,but also to increase the accumulations of total polyphenol and proanthocyanidin.SOD,POD,CAT,PAL,C4 Hin the adventitious bud responded the La treatment rapidly.The activities of SOD,POD,CAT,PAL,C4 Hincreased first,and then decreased.The peak values of the corresponding enzymes were 1.79,1.51,1.33,3.98,and 3.61 times more than the control.CTS,MeJA and La could act as potent elicitors to induce the defense responses to stimulate secondary metabolite production in P.koraiensis.
The objectives of this study were to investigate the induction of pine polyphenols biosynthesis by three exogenous substances including chitosan(CTS),methyl jasmonate(MeJA)and lanthanum nitrate(La).Adventitious bud of Pinus koraiensis was used as the material,the effects of three exogenous substances were examined on the growth,the pine polyphenols accumulation,the key biosynthesis enzymes and the defense enzymes.The results showed that CTS,MeJA and La had the abilities to facilitate the syntheses of pine polyphenol with different efficiencies,in which La presented the most significant effects.In the condition of examination concentration,La was able not only to facilitate a growth of adventitious bud,but also to increase the accumulations of total polyphenol and proanthocyanidin.SOD,POD,CAT,PAL,C4 Hin the adventitious bud responded the La treatment rapidly.The activities of SOD,POD,CAT,PAL,C4 Hincreased first,and then decreased.The peak values of the corresponding enzymes were 1.79,1.51,1.33,3.98,and 3.61 times more than the control.CTS,MeJA and La could act as potent elicitors to induce the defense responses to stimulate secondary metabolite production in P.koraiensis.
引文
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[2]JEREZ M,TOURINO S,SINEIRO J,et al.Procyanidins from pine bark.Relationship between structure,composition and antiradical activity[J].Food Chemistry,2007(104):518-527.
[3]LUO X Y.Supplementing conventional treatment with pycnogenol(R)may improve hepatitis C virus-associated type 2diabetes:a mini review[J].Journal of Clinical and Translational Hepatology,2016(3):228-233.
[4]TMEN,AKKOL E K,TATAN H,et al.Research on the antioxidant,wound healing,and anti-inflammatory activities and the phytochemical composition of maritime pine(Pinus pinaster Ait)[J].Journal of Ethnopharmacology,2018(211):235-246
[5]BELCARO G,CESARONE M R,GENVESI D,et al.Pycnogenol may alleviate adverse effects in oncologic treatment[J].Panminerva Medica,2008(50):227-234.
[6]GUNEL C,DEMIRCI B,ERYILMAZ A,et al.Inhibitory effect of pycnogenol on airway inflammation in ovalbumin-Induced allergic rhinitis[J].Balkan Medical Journal,2016,33(6):620-626.
[7]SUN D,LU X,HU Y,et al.Methyl jasmonate induced defense responses increase resistance to Fusarium oxysporum f.sp.cubense race 4in banana[J].Scientia Horticulturae,2013(164):484-491.
[8]DOKHANIEH A Y,AGHDAM M S,FARD J,et al.Postharvest salicylic acid treatment enhances antioxidant potential of cornelian cherry fruit[J].Scientia Horticulturae,2013(154):31-36.
[9]王小菲,高文强,刘建锋,等.植物防御策略及其环境驱动机制[J].生态学杂志,2015,34(12):3542-3552.
[10]LIU X,CHI H,YUE M,et al.The regulation of exogenous jasmonic acid on UV-B stress tolerance in wheat[J].Journal of Plant Growth Regulation,2012(3):436-447.
[11]YU H,LIU X,GAO S,et al.Molecular cloning and functional characterization of a phenylalanine ammonia-lyase from liverwort Plagiochasma appendiculatum[J].Plant Cell,Tissue and Organ Culture,2014,117(2):265-277.
[12]XING B C,YANG D,LIU F L,et al.Phenolic acid production is more effectively enhanced than tanshinone production by methyl jasmonate in Salvia miltiorrhiza hairy roots[J].Plant Cell,Tissue and Organ Culture,2018,134(1):119-129.
[13]ALSCHER R G,ERTURK N,HEATH L S.Role of superoxide dismutases(SODs)in controlling oxidative stress in plants[J].Journal of Experimental Botany,2002,53(372):1331-1341.
[14]刘玉莲,左存武,车飞,等.日灼后苹果果皮保护机制研究[J].西北林学院学报,2017(6):18-24.LIU Y L,ZUO C W,CHE F,et al.Defense mechanism of sunburn for apple peel[J].Journal of Northwest Forestry U-niversity,2017(6):18-24.(in Chinese)
[15]CHELIKANI P,FITA I,LOEWEN P C.Diversity of structures and properties among catalases[J].Cellular and Molecular Life Sciences,2004,61(2):192-208.
[16]XU H Y,LI X J,WANG S M,et al.Oligosaccharide elicitor prepared from Salecan triggers the defense responses of Arabidopsis thaliana Col0against botrytis cinerea infection[J].World Journal of Microbiology and Biotechnology,2017,(33):165.
[17]SUDHA G,RAVISHANKAR G A.Ravishankar Involvement and interaction of various signaling compounds on the plant metabolic events during defense response,resistance to stress factors,formation of secondary metabolites and their molecular aspects[J].Plant Cell,Tissue and Organ Culture,2002,71(3):181-212.
[18]周桂,靳晓芸,邓光辉,等.壳寡糖诱导甘蔗叶多酚与防御酶活性的变化[J].南方农业学报,2011,42(8):874-877.ZHOU G,JIN X Y,DENG G H,et al.Chitosan oligosaccharide induced changes in polyphenols and defense enzymes activities in sugarcane leaves[J].Guangxi Agricultural Sciences,2011,42(8):874-877.(in Chinese)
[19]姜照伟,翁伯琦,黄元仿,等.施用稀土元素镧对南非马唐生长及若干生理特性的影响[J].植物营养与肥料学报,2008,14(4):713-720.JIANG Z W,WENG B Q,HUANG Y F,et al.Effects of applied rare earth elements lanthanum on the growth and some physiological characteristics of digitaria smutsii[J].Plant Nutrition and Fertilizer Science,2008,14(4):713-720.(in Chinese)
[20]高海波,沈应柏.水杨酸甲酯、苯骈噻唑及茉莉酸甲酯对合作杨防御物质的影响[J].西北林学院学报,2007,22(6):32-35.GAO H B,SHEN Y B.Effects of BTH,MeSA and MeJA on Defensive Components of Populus simonii×P.pyramidalis[J].Journal of Northwest Forestry University,2007,22(6):32-35.(in Chinese)
[21]CHENG X Y,ZHOU H Y,CUI X,et al.Improvement of phenylethanoid glycosides biosynthesis in Cistanche deserticola cell suspension cultures by chitosan elicitor[J].Journal of Biotechnology,2006,121(2):253-260.
[22]CHONG T M,ABDULLAH M A,FADZILLAH N M,et al.Jasmonic acid elicitation of anthraquinones with some associated enzymic and non-enzymic antioxidant responses in Morinda elliptica[J].Enzyme and Microbial Technology,2005,36(4):469-477.
[23]DONG J,WAN G,LIANG Z.Accumulation of salicylic acidinduced phenolic compounds and raised activities of secondary metabolic and antioxidative enzymes in Salvia miltiorrhiza cell culture[J].Journal of Biotechnology,2010,148(2):99-104.
[24]杨英,郑辉,李赟,等.茉莉酸甲酯与二氢茉莉酮酸甲酯对悬浮培养的甘草细胞生长和黄酮积累的影响[J].植物生理学通讯,2008,44(5):903-906.
[25]董诚明,曹利华,苏秀红,等.稀土元素镧和铈对冬凌草再生植株生长及次生代谢产物的影响[J].广西植物,2015,35(3):437-441.