肿瘤特异性蛋白70用于乳腺肿瘤良恶性鉴别
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摘要
目的:探讨肿瘤特异性蛋白70(tumor specific protein 70,SP70)在乳腺肿瘤组织及血液中的表达情况及其对乳腺肿瘤良恶性鉴别的意义。方法:乳腺肿瘤患者125例(乳腺癌65例、良性病变60例),免疫组化法检测切片中SP70的表达。ELISA检测120例乳腺肿瘤患者(58例乳腺癌、62例乳腺良性疾病)和60例健康体检者血清中SP70蛋白的水平。结果:SP70在乳腺癌组织中的表达阳性率(55.38%)高于乳腺良性病变(15.00%),差异有统计学意义(χ2=23.846,P<0.001)。乳腺癌患者血清中SP70的水平显著高于乳腺良性疾病患者和健康对照,差异有统计学意义(F=11.884,P<0.001)。SP70在血清中的敏感性高于CA153(53.45%vs.44.82%),且血清中SP70和CA153的表达无显著相关性(r=0.132,P=0.324),两者联合能够明显提高检测敏感性(84.38%)。结论:检测乳腺肿瘤组织和血液中SP70的表达对其良恶性的鉴别有重要临床价值。
Objective: To investigate the expression of tumor specific protein 70 (SP70) in breast cancer tissues and blood to evaluate whether SP70 could differentiate the benign breast tumor and malignant breast tumor.Methods: The SP70 expressions of paraffin sections in the tissues of 65 malignant breast tumors and 60 benign breast tumors were studied by immunohistochemistry (IHC) technique.The levels of SP70 in the serum of 58 malignant breast tumors,62 benign breast tumors and 60 healthy subjects were studied by enzyme-linked immuno sorbent assay (ELISA).Results: The expression positive rate of SP70 was 55.38% in the tissues of malignant breast tumors,whereas,only 15.00% in benign breast tumors.The difference was statistically significant (χ2=23.846,P<0.001).The serum SP70 level of patients with malignant breast tumors was significantly higher than that in patients with benign breast tumors and healthy controls (F=11.884,P<0.001).The sensitivity of serum SP70 for diagnosis of malignant breast tumors was significantly higher than that of serum CA153 (53.45% vs.44.82%),a significant association between serum SP70 and serum CA153 had not been found (r=0.132,P=0.324).The combine of serum SP70 and serum CA153 could significantly improve the sensitivity (84.38%).Conclusion: SP70 may be used to differentiate diagnosis of malignant breast tumors and benign breast diseases.
Objective: To investigate the expression of tumor specific protein 70 (SP70) in breast cancer tissues and blood to evaluate whether SP70 could differentiate the benign breast tumor and malignant breast tumor.Methods: The SP70 expressions of paraffin sections in the tissues of 65 malignant breast tumors and 60 benign breast tumors were studied by immunohistochemistry (IHC) technique.The levels of SP70 in the serum of 58 malignant breast tumors,62 benign breast tumors and 60 healthy subjects were studied by enzyme-linked immuno sorbent assay (ELISA).Results: The expression positive rate of SP70 was 55.38% in the tissues of malignant breast tumors,whereas,only 15.00% in benign breast tumors.The difference was statistically significant (χ2=23.846,P<0.001).The serum SP70 level of patients with malignant breast tumors was significantly higher than that in patients with benign breast tumors and healthy controls (F=11.884,P<0.001).The sensitivity of serum SP70 for diagnosis of malignant breast tumors was significantly higher than that of serum CA153 (53.45% vs.44.82%),a significant association between serum SP70 and serum CA153 had not been found (r=0.132,P=0.324).The combine of serum SP70 and serum CA153 could significantly improve the sensitivity (84.38%).Conclusion: SP70 may be used to differentiate diagnosis of malignant breast tumors and benign breast diseases.
引文
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[2]Sardanelli F,Podo F,Santoro F,et al.Multicenter surveillance of women at high genetic breast cancer risk using mammography,ultrasonography,and contrast-enhanced magnetic resonance imaging(the high breast cancer risk Italian 1 study):final results[J].Invest Radiol,2011,46(2):94-105
[3]Nadler M,Al-Attar H,Warner E,et al.MRI surveillance for women with dense breasts and a previous breast cancer and/or high risk lesion[J].Breast,2017,34:77-82
[4]Marmot MG,Altman DG,Cameron DA,et al.The benefits and harms of breast cancer screening:an independent review[J].Br J Cancer,2013,108:2205-2240
[5]Hasanzadeh A,Mesrian Tanha H,Ghaedi K,et al.Aberrant expression of mi R-9 in benign and malignant breast tumors[J].Mol Cell Probes,2016,30(5):279-284
[6]韩月,王芳,徐婷,等.NJ001特异性抗原在肺腺癌中的表达及临床意义[J].中华检验医学杂志,2013,36(10):895-898
[7]Pan SY,Wang F,Huang PJ,et al.The study on newly developed Mc Ab NJ001 specific to non-small cell lung cancer and its biological characteristics[J].PLo S One,2012,7(3):e33009
[8]彭婪,潘世扬,王芳,等.非小细胞肺癌患者血清中SP70的检测及其临床意义[J].中华检验医学杂志,2012,35(6):554-558
[9]吴蕾,李大千,荆俊鹏,等.NJ001特异性抗原在胰腺癌组织中表达的初步研究[J].南京医科大学学报(自然科学版),2015,35(8):1127-1130
[10]李月梅,荆俊鹏,吴蕾,等.SP70在卵巢癌组织中的表达及临床病理意义[J].南京医科大学学报(自然科学版),2016,36(1):65-68
[11]Catsburg C,Miller AB,Rohan TE.Adherence to cancer prevention guidelines and risk of breast cancer[J].Int J Cancer,2014,135(10):2444-2452
[12]Ferlay J,Shin HR,Bray F,et al.Estimates of worldwide burden of cancer in 2008:GLOBOCAN 2008[J].Int J Cancer,2010,127(12):2893-2917
[13]Yip CH,Cazap E,Anderson BO,et al.Breast cancer management in middle-resource countries(MRCs):consensus statement from the Breast Health Global Initiative[J].Breast,2011,20(Suppl 2):S12-19
[14]Dinkel HP,Trusen A,Gassel AM,et al.Predictive value of galactographic patterns for benign and malignant neoplasms of the breast in patients with nipple discharge[J].Br J Radiol,2000,73(871):706-714
[15]Zhang Y,Zhang K,Luo Z,et al.Circulating long noncoding HOX transcript antisense intergenic ribonucleic acid in plasma as a potential biomarker for diagnosis of breast cancer[J].Thorac Cancer,2016,7(6):627-632
[16]杨瑞霞,潘世扬,王芳,等.良恶性胸腔积液鉴别中SP70检测的临床意义[J].中华检验医学杂志,2012,35(12):1150-1154