2015-2016年北京市顺义区人源性副溶血性弧菌耐药性分析
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摘要
目的通过对46株腹泻病例中分离的副溶血性弧菌进行药敏实验,分析其耐药状况,并评估其耐药风险。方法使用肉汤稀释法对46株副溶血性弧菌进行药敏实验,分析其对11类抗生素的药敏定量结果。结果 46株副溶血性弧菌主要耐受的抗生素是氨苄西林,耐药率为6.52%;其次为头孢西丁,耐药率是2.17%。除耐药外,氨苄西林药敏结果的中敏率为89.13%,敏感率为4.35%;头孢西丁的中敏率为0,敏感率为97.83%;舒巴坦的中敏率为2.17%,敏感率为97.83%。46株副溶血性弧菌对其他8种抗生素耐药率均为0。结论北京市顺义区人源性副溶血弧菌只对氨苄西林和头孢西丁耐药;头孢西丁的耐药率有所升高,舒巴坦出现中敏情况,有耐药发生趋势,临床上需注意β-内酰胺类抗生素的合理使用。
Objective To analyze the drug resistance of vibrio parahaemolyticus isolated from 46 cases of diarrhea, and to evaluate the risk of drug resistance. Methods 46 strains of vibrio parahaemolyticus were tested by broth dilution method. The sensitive quantitative result of drug resistance, intermediate sensitive or sensitive to all kinds of antibiotics were analyzed. Results Among 46 strains of vibrio parahaemolyticus, 6.52% were resistant to ampicillin, and 2.17%were resistant to cefoxitin. In addition to ampicillin resistance, drug sensitivity result of the intermediary rate was 89.13%, the sensitivity rate was 4.35%; cefoxitin intermediary rate was 0, the sensitivity rate was 97.83%; sulbactam intermediate rate was 2.17%, the sensitivity rate was 97.83%. None of 46 vibrio parahaemolyticus was resistant to the other eight antibiotics. Conclusion In Shunyi district, infectious vibrio parahaemolyticus is only resistant to ampicillin and cefoxitin, the drug resistance is not very serious; The increasing rate of cefoxitin resistance and the emergence of Shubatan as an intermediary suggested that more attention should be paid to the clinical use of beta lactam antibiotics.
Objective To analyze the drug resistance of vibrio parahaemolyticus isolated from 46 cases of diarrhea, and to evaluate the risk of drug resistance. Methods 46 strains of vibrio parahaemolyticus were tested by broth dilution method. The sensitive quantitative result of drug resistance, intermediate sensitive or sensitive to all kinds of antibiotics were analyzed. Results Among 46 strains of vibrio parahaemolyticus, 6.52% were resistant to ampicillin, and 2.17%were resistant to cefoxitin. In addition to ampicillin resistance, drug sensitivity result of the intermediary rate was 89.13%, the sensitivity rate was 4.35%; cefoxitin intermediary rate was 0, the sensitivity rate was 97.83%; sulbactam intermediate rate was 2.17%, the sensitivity rate was 97.83%. None of 46 vibrio parahaemolyticus was resistant to the other eight antibiotics. Conclusion In Shunyi district, infectious vibrio parahaemolyticus is only resistant to ampicillin and cefoxitin, the drug resistance is not very serious; The increasing rate of cefoxitin resistance and the emergence of Shubatan as an intermediary suggested that more attention should be paid to the clinical use of beta lactam antibiotics.
引文
[1]何珊娜,陈敏,金海英.病人标本检出副溶血性弧菌生物学特征及耐药性研究[J].中国消毒学杂志,2015,32(9):877-878.
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[3]朱敏,梅玲玲,张俊彦,等.120株副溶血性弧菌的血清群和耐药性分析[J].中国卫生检验杂志,2008,18(11):2292-2294.
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[5]吴南卫,李祥,邓瑶,等.三亚市食物中毒中人感染副溶血性弧菌的毒力基因及耐药性研究[J].现代预防医学,2012,39(16):4221-4222,4225.
[6]商晓春,詹丽,帅慧群,等.杭州市下城区副溶血性弧菌菌群型分布、毒力基因及耐药性研究[J].中国卫生检验杂志,2011,21(11):2779-2782.
[7]宋曼丹,严纪文,朱海明,等.不同来源副溶血性弧菌分离株的耐药性和毒力分析[J].中国卫生检验杂志,2011,21(11):2785-2787.
[8]张爽,李颖,张彦春,等.2013-2016年北京市顺义区副溶血性弧菌感染性腹泻流行特征分析[J].预防医学情报杂志,2018,34(10):1306-1309.
[9]王秋亚,朱联辉,张兰荣,等.2009-2011年北京市通州区分离的副溶血性弧菌的耐药谱分析[J].生物技术通讯,2012,23(3):333-336.
[10]付溥博,张西萌,马丹,等.2008-2017年间北京进出口水产品中副溶血性弧菌耐药性分析[J].食品安全质量检测学报,2018,9(14):3835-3840.
[11]王彦波,李颖,李湛,等.2016年北京市顺义区食源性疾病主动监测实验室检测结果分析[J].预防医学情报杂志,2018,34(3):344-348.
[12]刘艳洁,魏耀华,侯申岩.副溶血弧菌致病性及耐药性分析[J].中国误诊学杂志,2007,7(3):467-468.
[13]赖植发,张勇,罗伟燕,等.副溶血性弧菌食物中毒分离株毒力基因检测及耐药性的调查[J].热带医学杂志,2009,9(8):882-883,915.
[14]严寒秋,黄瑛,吕冰,等.不同来源副溶血弧菌耐药性分析[J].中国卫生检验杂志,2015,25(21):3765-3768,3771.
[15]白瑶,叶淑瑶,闫韶飞,等.2015年我国食源性副溶血性弧菌毒力基因及耐药特征研究[J].食品安全质量检测学报,2017,8(6):2018-2024.
[16]曲梅,张新,刘桂荣,等.北京市副溶血弧菌病原学和分子流行病学特征分析[J].中华流行病学杂志,2011,32(12):1255-1258.DOI:10.3760/cma.j.issn.0254-6450.2011.12.017.
[17]王云霞,刘颖,刘海波,等.北京市房山区腹泻病例分离副溶血性弧菌的分子流行病学特征[J].职业与健康,2018,34(14):1909-1912,1916.
[2]江艳华,姚琳,李风铃,等.副溶血性弧菌的耐药状况及耐药机制研究进展[J].中国渔业质量与标准,2013,3(4):96-102.
[3]朱敏,梅玲玲,张俊彦,等.120株副溶血性弧菌的血清群和耐药性分析[J].中国卫生检验杂志,2008,18(11):2292-2294.
[4]CHAO G,ZHOU X,JIAO X,et al.Prevalence and antimicrobial resistance of foodborne pathogens isolated from food products in China.[J].Foodborne Pathog Dis,2007,4(3):277-284.
[5]吴南卫,李祥,邓瑶,等.三亚市食物中毒中人感染副溶血性弧菌的毒力基因及耐药性研究[J].现代预防医学,2012,39(16):4221-4222,4225.
[6]商晓春,詹丽,帅慧群,等.杭州市下城区副溶血性弧菌菌群型分布、毒力基因及耐药性研究[J].中国卫生检验杂志,2011,21(11):2779-2782.
[7]宋曼丹,严纪文,朱海明,等.不同来源副溶血性弧菌分离株的耐药性和毒力分析[J].中国卫生检验杂志,2011,21(11):2785-2787.
[8]张爽,李颖,张彦春,等.2013-2016年北京市顺义区副溶血性弧菌感染性腹泻流行特征分析[J].预防医学情报杂志,2018,34(10):1306-1309.
[9]王秋亚,朱联辉,张兰荣,等.2009-2011年北京市通州区分离的副溶血性弧菌的耐药谱分析[J].生物技术通讯,2012,23(3):333-336.
[10]付溥博,张西萌,马丹,等.2008-2017年间北京进出口水产品中副溶血性弧菌耐药性分析[J].食品安全质量检测学报,2018,9(14):3835-3840.
[11]王彦波,李颖,李湛,等.2016年北京市顺义区食源性疾病主动监测实验室检测结果分析[J].预防医学情报杂志,2018,34(3):344-348.
[12]刘艳洁,魏耀华,侯申岩.副溶血弧菌致病性及耐药性分析[J].中国误诊学杂志,2007,7(3):467-468.
[13]赖植发,张勇,罗伟燕,等.副溶血性弧菌食物中毒分离株毒力基因检测及耐药性的调查[J].热带医学杂志,2009,9(8):882-883,915.
[14]严寒秋,黄瑛,吕冰,等.不同来源副溶血弧菌耐药性分析[J].中国卫生检验杂志,2015,25(21):3765-3768,3771.
[15]白瑶,叶淑瑶,闫韶飞,等.2015年我国食源性副溶血性弧菌毒力基因及耐药特征研究[J].食品安全质量检测学报,2017,8(6):2018-2024.
[16]曲梅,张新,刘桂荣,等.北京市副溶血弧菌病原学和分子流行病学特征分析[J].中华流行病学杂志,2011,32(12):1255-1258.DOI:10.3760/cma.j.issn.0254-6450.2011.12.017.
[17]王云霞,刘颖,刘海波,等.北京市房山区腹泻病例分离副溶血性弧菌的分子流行病学特征[J].职业与健康,2018,34(14):1909-1912,1916.