不同碱煮时间对玉米快速提取DNA效果的影响
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摘要
随着SSR分子标记技术在玉米室内测纯、一致性及真实性鉴定等方面的应用,该技术已经逐渐成为种子企业质量检测后控的基本手段。但由于其检测量大,准确性要求高的特点,使其对DNA提取质量的要求增加。该文以快速DNA提取方法为基础,在普通的实验条件下,设置4种不同沸水浴的碱煮时间,比较提取DNA后的电泳效果,寻求适合一般种子企业实验室SSR分子试验要求的DNA快速提取方法,为种子企业的快速质量测定奠定基础。
With the use of SSR technology in the aspects of corn chamber test purity,consistency and authenticity identification,this technology has gradually become the seed enterprise quality testing after the control of the basic means.Due to its high detection quantity and high accuracy,the requirement of DNA extraction quality is increased.In this paper,based on the previous human rapid DNA extraction method,under the general experimental conditions of seed enterprises,four different boiling water bath alkali boiling time was set to compare the effect of DNA extraction after electrophoresis,to find a suitable method for rapid DNA extraction in the laboratory of seed enterprises,so as to lay a foundation for rapid quality determination of seed enterprises.
With the use of SSR technology in the aspects of corn chamber test purity,consistency and authenticity identification,this technology has gradually become the seed enterprise quality testing after the control of the basic means.Due to its high detection quantity and high accuracy,the requirement of DNA extraction quality is increased.In this paper,based on the previous human rapid DNA extraction method,under the general experimental conditions of seed enterprises,four different boiling water bath alkali boiling time was set to compare the effect of DNA extraction after electrophoresis,to find a suitable method for rapid DNA extraction in the laboratory of seed enterprises,so as to lay a foundation for rapid quality determination of seed enterprises.
引文
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[10]戴剑,张云辉.SSR扩增产物检测方法比较及其实验操作注意事项[J].种子,2013,32(9):120-123.
[2]王艳,李韶山.植物总DNA样品的快速制备[J].激光生物学报,2000,9(1):79-80.
[3]郭景伦,赵久然,王凤格.适用于SSR分子标记的玉米单粒种子DNA快速提取新方法[J].玉米科学,2005,13(2):16-17.
[4]郭景伦,王斌.玉米单粒种子DNA提取新方法[J].农业新技术,1997(2):1-2.
[5]易红梅,张芳,王凤格,等.不同取样方式对DNA快速提取效果的影响[J].华北农学报,2007,22(5):114-116.
[6]闫燕,许文涛,苏春元,等.平菇基因组DNA提取方法的研究[J].食品工业科技,2011(9):190-193.
[7]曾乐平,周洪波,黄菊芳.一种经济、简单的微生物基因组DNA的提取方法[J].生态环境学报,2005,14(6):945-946.
[8]刘泳,杨官品.碱煮法:一种制备海洋浮游生物PCR模板DNA的实用方法[J].海洋科学,2004,28(7):1-3.
[9]李心平,马福丽,高连兴.玉米种子的机械损伤对其发芽率的影响[J].农机化研究,2009,31(3):34-35.
[10]戴剑,张云辉.SSR扩增产物检测方法比较及其实验操作注意事项[J].种子,2013,32(9):120-123.