补肝健腰方对腰椎间盘退变大鼠椎间盘MMP-3、TIMP-1 mRNA表达的影响
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摘要
目的:观察补肝健腰方对腰椎间盘退变大鼠椎间盘MMP-3、TIMP-1 mRNA表达的影响。方法:将SD大鼠75只,随机分为假手术组、模型组、腰痹通组、强力霉素组、补肝健腰组,每组各15只,成功制备大鼠腰椎间盘退变模型,分别给与腰痹通胶囊、强力霉素、补肝健腰方药物灌胃,检测各组干预前,干预第20天、第40天时椎间盘MMP-3、TIMP-1 mRNA表达,进行对比。结果:干预第20天,第40天,腰痹通组、强力霉素组、补肝健腰组椎间盘MMP-3、TIMP-1 mRNA表达较模型组下降,差异有统计学意义(P<0.05),腰痹通组、强力霉素组、补肝健腰组在干预第20天,第40天时椎间盘MMP-3、TIMP-1 mRNA表达水平,分别与本组干预前比较,差异有统计学意义(P<0.05),补肝健腰组在以上各时间点与腰痹通组、强力霉素组比较,差异无统计学意义(P>0.05)。结论:补肝健腰方能降低腰椎间盘退变模型大鼠椎间盘MMP-3、TIMP-1 mRNA表达,这可能为防治椎间盘退变疾病的作用机理。
Objective: To observe the effect of Bugan Jianyao decoction(补肝健腰方, BGJYD) on MMP-3,TIMP-1 mRNA expression in lumbar disc degeneration rat model. Methods: The 75 SD rats were randomly divided into sham-operated group, model group, Yaobitong(腰痹通) group, doxycycline group, BGJYD group, each group with 15 rats. After rat lumbar disc degeneration model was prepared successfully, BGJYD group was given the BGJYD intragastric administration, while Yaobitong group, doxycycline group was given Yaobitong capsule and doxycycline respectively, sham-operated group and model group were intragastrically administrated with the same dose of normal saline. MMP-3, TIMP-1 mRNA expression were detected at the moment of the experimental intervention, 20 thday and 40 thday. Results: At the moment of 20 thday and 40 thday, compared with the model group, MMP-3, TIMP-1 mRNA expression of Yaobitong group, doxycycline group, BGJYD group decreased(P<0.05). Compared with that of the group before intervention, the changes of expression level of MMP-3 and TIMP-1 mRNA in intervertebral disc of Yaobitong group, doxycycline group and BGJYD group at 20 th day and 40 thday were statistically significant(P<0.05). Compared with Yaobitong group and doxycycline group in the above time points, BGJYD group had no significant difference(P>0.05). Conclusions: BGJYD could effectively lower MMP-3, TIMP-1 mRNA expression of rat lumbar disc degeneration model, it may be the mechanism for the treatment of lumbar disc degeneration.
Objective: To observe the effect of Bugan Jianyao decoction(补肝健腰方, BGJYD) on MMP-3,TIMP-1 mRNA expression in lumbar disc degeneration rat model. Methods: The 75 SD rats were randomly divided into sham-operated group, model group, Yaobitong(腰痹通) group, doxycycline group, BGJYD group, each group with 15 rats. After rat lumbar disc degeneration model was prepared successfully, BGJYD group was given the BGJYD intragastric administration, while Yaobitong group, doxycycline group was given Yaobitong capsule and doxycycline respectively, sham-operated group and model group were intragastrically administrated with the same dose of normal saline. MMP-3, TIMP-1 mRNA expression were detected at the moment of the experimental intervention, 20 thday and 40 thday. Results: At the moment of 20 thday and 40 thday, compared with the model group, MMP-3, TIMP-1 mRNA expression of Yaobitong group, doxycycline group, BGJYD group decreased(P<0.05). Compared with that of the group before intervention, the changes of expression level of MMP-3 and TIMP-1 mRNA in intervertebral disc of Yaobitong group, doxycycline group and BGJYD group at 20 th day and 40 thday were statistically significant(P<0.05). Compared with Yaobitong group and doxycycline group in the above time points, BGJYD group had no significant difference(P>0.05). Conclusions: BGJYD could effectively lower MMP-3, TIMP-1 mRNA expression of rat lumbar disc degeneration model, it may be the mechanism for the treatment of lumbar disc degeneration.
引文
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[2]仇湘中,张信成,严敏,等.补肝健腰方对腰椎间盘退变大鼠髓核超微结构的影响[J].中医药导报,2015,21(19):19-22.
[3]仇湘中,蒋盛昶,张信成,等.补肝健腰方对腰椎间盘退变大鼠髓核组织TNF-α、IL-1β干预作用的研究[J].湖南中医药大学学报,2015,35(4):17-23.
[4]崔力扬,刘尚礼,丁悦,等.大鼠腰椎间盘针刺退变模型的建立[J].中国矫形外科杂志,2007,15(13):1008-1011.
[5]魏伟,吴希美,李元建.药理实验方法学[M].北京:人民卫生出版社,2010:1698.
[6]王涛,康汇,李红川.腰椎间盘突出患者组织MMP及TIMP指标的变化研究[J].湖南师范大学学报(医学版),2015,12(6):129-131.
[7]原野,赵静,李永民,等.腰椎间盘退变组织中NFκ-B、MMP-1/TIMP-1的表达及意义[J].山东医药,2011,51(19):4-5.
[8]魏春,徐杰.腰椎间盘突出症患者外周血白细胞介素-1β、前列腺素E2及基质金属蛋白酶-3水平变化[J].新乡医学院学报,2017,34(1):76-78.
[9]李曾昊,李宁宁,肖荣驰.强力霉素对退变腰椎间盘中MMP-3表达水平的调控[J].湖南中医药大学学报,2010,30(4):64-66.
[10]仇湘中,黎玉林.腰椎间盘突出症的中西药治疗对比观察[J].中国骨伤,1998,11(3):71.
[11]仇湘中,张信成,蒋盛昶,等.补肝健腰方对腰椎退变大鼠椎间盘组织AQP1表达的影响[J].新中医,2014,46(12):191-193.