一种荧光增强型硫化氢探针的设计合成及应用
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摘要
通过3,4-二甲氧基苯胺与2-硝基苯甲醛一步反应,合成了一种荧光增强型的硫化氢探针S2,并通过核磁和质谱确定了S2的结构。紫外分光光度计测试表明,探针S2与硫化钠发生了反应。在PBS缓冲液中,10μmol/L探针S2与硫化钠反应后,通过荧光分光光度计进行荧光信号的检测。结果表明,探针S2可在8 min与硫化钠响应完全,随着硫化钠浓度的增加,探针S2在390 nm处的荧光值增加,且探针S2的荧光信号比值(F/F0)与硫化钠在0～8μmol/L的范围内呈线性关系,检出限为0. 20μmol/L。在与其他分析物的响应中,探针S2的选择性也很好。在自来水中通过加标回收实验,回收率在83. 3%～99. 4%之间。
A fluorescence enhanced hydrogen sulfide probe S2 was synthesized by the one-step reaction of 3,4-dimethoxyaniline and 2-nitrobenzaldehyde,and the structure of S2 was determined by NMR and MS. UV spectrophotometer showed that the probe S2 was reacted with sodium sulfide. In the PBS buffer,10 μmol/L probe S2 reacted with sodium sulfide and was detected by fluorescence spectrophotometer.The results showed that the probe S2 can respond completely at 8 min with sodium sulfide. With the increase of sodium sulfide concentration,the fluorescence value of the probe S2 at 390 nm increases,and the fluorescence signal ratio( F/F0) of the probe S2 was linearly related to the sodium sulfide in the range of 0 ～ 8 μmol/L,and the detection limit was 0. 20 μmol/L. The selectivity of probe S2 was also good in response to other analytes. The recovery rate was 83. 3% to 99. 4% in tap water.
A fluorescence enhanced hydrogen sulfide probe S2 was synthesized by the one-step reaction of 3,4-dimethoxyaniline and 2-nitrobenzaldehyde,and the structure of S2 was determined by NMR and MS. UV spectrophotometer showed that the probe S2 was reacted with sodium sulfide. In the PBS buffer,10 μmol/L probe S2 reacted with sodium sulfide and was detected by fluorescence spectrophotometer.The results showed that the probe S2 can respond completely at 8 min with sodium sulfide. With the increase of sodium sulfide concentration,the fluorescence value of the probe S2 at 390 nm increases,and the fluorescence signal ratio( F/F0) of the probe S2 was linearly related to the sodium sulfide in the range of 0 ～ 8 μmol/L,and the detection limit was 0. 20 μmol/L. The selectivity of probe S2 was also good in response to other analytes. The recovery rate was 83. 3% to 99. 4% in tap water.
引文
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[2] Donnarumma E,Trivedi R K,Lefer D J. Compr Physiol,2017,7(2):583
[3] Kondo K,Bhushan S,King A L,Prabhu S D,Hamid T,Koenig S,Murohara T,Predmore B L,Gabriel G S,Gabriel G J,Wang R,Karusula N,Nicholson C K,Calvert J W,Lefer D J. Circulation,2013,127(10):1116
[4] Yang R,Jia Q,Liu X F,Gao Q,Wang L,Ma S F. Chinese Journal of Applied Physiology,2016,32(1):8杨锐,贾强,刘小粉,高琴,王蕾,马善峰.中国应用生理学杂志,2016,32(1):8
[5] Han W Z,Cai S L. Practical Journal of Medicine&Pharmacy,2007,24(5):513韩文章,蔡尚郎.实用医药杂志,2007,24(5):513
[6] Elrod J W,Calvert J W,Morrison J,Doeller J E,Kraus D W,Tao L,Jiao X,Scalia R,Kiss L,Szabo C,Kimura H,Chow C W,Lefer D J. Proc Nat Acad Sci USA,2007,104(39):15560
[7] Pandey S K,Kim K H. Environ Sci Technol,2009,43(9):3020
[8] Furne J,Saeed A,Levitt M D. Am J Physiol-Reg I,2008,295(5):1479
[9] Lawrence N S,Davis J,Jiang L,Jones T G,Davies S N,Compton R G. Electroanal,2000,12(18):1453
[10] Sun Z G,Li Z,Yuan D D,Gao J F,Lin L,Lin J,Zhu M L,Makawana J A,Qian Y,Zhu H L. Chem Pharm Bull Tokyo,2016,64(1):27
[11] Zhang L,Zheng X E,Zou F,Shang Y,Meng W,Lai E,Xu Z,Liu Y,Zhao J. Sci Rep,2016,6:18868
[12] Yan Y Y,Zhang L,Wu Q,Yu M Y,Yang C,Lu Z W,Zhang J,Zhao J Y,Tang H M,Wang S W,Feng G C,Wei W,Hong M,Zhao J. SCIENTIA SINICA Chimica,2015,45(8):812严义勇,张玲,吴谦,余孟颖,杨超,陈柱文,张健,赵镜一,唐红梅,王双卫,冯耿超,魏炜,洪梅,赵劲.中国科学:化学,2015,45(8):812
[13] Hou P,Dong Y J,Li S,Xu F. Chin. J. Anal. Lab.,2017,36(10):1206侯鹏,董玉晶,李爽,许凤.分析试验室,2017,36(10):1206
[14] Liu C H,Liu B,Qi F P,Wang H Y. Journal of Hunan City University(Natural Science),2017,26(3):65刘长辉,刘波,齐风佩,王海洋.湖南城市学院学报(自然科学版),2017,26(3):65
[15] Zhang K,Zhang J,Xi Z,Li L Y,Gu X,Zhang Q Z,Yi L. J Chem Sci,2017,8(4):2776