手性钌配合物与DNA相互作用的电子吸收光谱研究
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摘要
目的合成三个手性钌配合物Δ-[Ru(bpy)_2(o-tFPIP)](PF_6)_2、Δ-[Ru(bpy)_2(p-tFPIP)](PF_6)_2、Δ-[Ru(bpy)_2(m-tFPIP)](PF_6)_2,研究这些配合物与DNA相互作用。方法用元素分析、质谱和核磁共振对配合物进行表征,并用电子吸收光谱研究钌配合物与DNA相互作用。结果三个手性钌配合物的电子吸收光谱均发生红移和减色效应,其中三氟甲基在对位取代的手性钌配合物减色效应最大。结论这些手性钌配合物通过插入方式与DNA结合,且三氟甲基在不同取代位置会影响配合物与DNA分子相互作用强弱。
Objective To synthesis three chiral ruthenium complexes Δ-[Ru(bpy)_2(o-tFPIP)](PF_6)_2、Δ-[Ru(bpy)_2(p-tFPIP)](PF_6)_2、Δ-[Ru(bpy)_2(m-tFPIP)](PF_6)_2, and study DNA binding properties of these chiral ruthenium complexes. Methods Three chiral ruthenium complexes has been characterized by elemental analysis, ES-MS and 1 HNMR, and the interactions of these complexes with DNA were investigated by electronic absorption spectra. Results The electronic absorption spectra of three chiral ruthenium complexes show red shift and hypochromic effect, of which Δ-[Ru(bpy)_2(p-tFPIP)](PF_6)_2 is the most effective. Conclusion The chiral ruthenium complex binds to DNA through intercalation mode, and the hypochromic effect of chiral ruthenium complexes is different,when these complexes have same substituent but different substitution position.
Objective To synthesis three chiral ruthenium complexes Δ-[Ru(bpy)_2(o-tFPIP)](PF_6)_2、Δ-[Ru(bpy)_2(p-tFPIP)](PF_6)_2、Δ-[Ru(bpy)_2(m-tFPIP)](PF_6)_2, and study DNA binding properties of these chiral ruthenium complexes. Methods Three chiral ruthenium complexes has been characterized by elemental analysis, ES-MS and 1 HNMR, and the interactions of these complexes with DNA were investigated by electronic absorption spectra. Results The electronic absorption spectra of three chiral ruthenium complexes show red shift and hypochromic effect, of which Δ-[Ru(bpy)_2(p-tFPIP)](PF_6)_2 is the most effective. Conclusion The chiral ruthenium complex binds to DNA through intercalation mode, and the hypochromic effect of chiral ruthenium complexes is different,when these complexes have same substituent but different substitution position.
引文
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[6]Wenjie Mei,Na Wang,Yunjun Liu,et al.Studies on cytotoxic and DNA-binding properties of two ruthenium(Ⅱ)complexes of a substituted phenanthroline ligand[J].Transition Met Chem,2008,33:499-503.
[7]张黔玲,刘剑洪,任祥忠,等.不同配体对钌多吡啶配合物与DNA的作用和荧光性质的影响[J].无机化学学报,2003,19(6):645-648.
[8]Kumar C V,Barton J K,Turro N J.Photophysics of Ruthenium Complexes Bound to Double Helical DNA[J].J.Am.Chem.Soc.,1985,107,5518-5523.
[9]王芳,张黔玲,刘剑洪,等.手性钌(Ⅱ)配合物对DNA的立体选择性断裂[J].高等学校化学学报,2004,25(11):2001-2003.
[10]R.H.Harsh,J.K.Barton,Novel dipyridophenazine complexes of ruthenium(II):exploring luminescent reporters of DNA[J]J.Am.Chem.Soc.,1992,114(15),5919-5925.
[2]Deng H,Xu H,Yang Y,et al.Synthesis,characterization,DNA-binding and cleavage studies of[Ru(bpy)2(actatp)]2+and[Ru(phen)2(actatp)]2+(actatp=acenaphthereno[1,2-b]-1,4,8,9-tetraazariphenylence)[J].J.Inorg.Biochem.,2003,97(2):207-214.
[3]张黔玲,刘剑洪,刘建忠,等.新型钌多吡啶的设计及其对配合物与DNA作用机制的影响[J].高等化学学报,2003,10,1754-1756.
[4]刘杰,计亮年,梅文杰.金属钌配合物的抗肿瘤活性及其作用机理.化学进展[J].2004,16(6):969-973.
[5]梅文杰,王娜,刘云军,等.手性钌配合物Δ-[Ru(bpy)2IPBP]2+的合成及其细胞毒作用[J].广东药学院学报,2004,20(5):451-452.
[6]Wenjie Mei,Na Wang,Yunjun Liu,et al.Studies on cytotoxic and DNA-binding properties of two ruthenium(Ⅱ)complexes of a substituted phenanthroline ligand[J].Transition Met Chem,2008,33:499-503.
[7]张黔玲,刘剑洪,任祥忠,等.不同配体对钌多吡啶配合物与DNA的作用和荧光性质的影响[J].无机化学学报,2003,19(6):645-648.
[8]Kumar C V,Barton J K,Turro N J.Photophysics of Ruthenium Complexes Bound to Double Helical DNA[J].J.Am.Chem.Soc.,1985,107,5518-5523.
[9]王芳,张黔玲,刘剑洪,等.手性钌(Ⅱ)配合物对DNA的立体选择性断裂[J].高等学校化学学报,2004,25(11):2001-2003.
[10]R.H.Harsh,J.K.Barton,Novel dipyridophenazine complexes of ruthenium(II):exploring luminescent reporters of DNA[J]J.Am.Chem.Soc.,1992,114(15),5919-5925.