蛋黄主要蛋白质研究进展
|
摘要
卵黄免疫球蛋白、蛋黄低密度脂蛋白、蛋黄高密度脂蛋白和卵黄高磷蛋白是蛋黄中的主要蛋白质,它们一方面为鸡胚胎发育提供重要的营养物质,另一方面,还具有抗菌活性、抗氧化活性等,为鸡胚发育提供保护。此外,蛋黄蛋白质的热凝胶特点、乳化特性等,为蛋黄及其相关产品提供了良好的质构特性,在食品加工中具有广泛应用。就蛋黄主要蛋白质的来源、在蛋黄中的组织状态、分子结构、功能特性以及分离纯化等进行综述,为蛋黄蛋白质的深入研究、应用和开发等提供参考。
The major egg yolk proteins, such as egg yolk immunoglobulin, egg yolk low density lipoprotein, egg yolk high density lipoprotein and phosvitin, not only provide important nutrients for chicken embryo development, but also protect the chicken embryo based on their antibacterial activity and antioxidant activity. Additionally, egg yolk proteins are also widely used in food processing because of their gelation and emulsification properties and giving the products a good texture. A review of the recent advances in the major egg yolk proteins was summarized, special focusing on the source, organizational status, molecular structure, functional properties, as well as the separation and purification of these proteins, and aiming to provide information for the study of egg yolk proteins and their application and development.
The major egg yolk proteins, such as egg yolk immunoglobulin, egg yolk low density lipoprotein, egg yolk high density lipoprotein and phosvitin, not only provide important nutrients for chicken embryo development, but also protect the chicken embryo based on their antibacterial activity and antioxidant activity. Additionally, egg yolk proteins are also widely used in food processing because of their gelation and emulsification properties and giving the products a good texture. A review of the recent advances in the major egg yolk proteins was summarized, special focusing on the source, organizational status, molecular structure, functional properties, as well as the separation and purification of these proteins, and aiming to provide information for the study of egg yolk proteins and their application and development.
引文
[1]王宁,马美湖.鸡蛋黄低密度脂蛋白理化及加工特性研究进展[J].中国粮油学报,2015,30(12):140-146.
[2]于智慧,王宁,蔡朝霞,等.鸡蛋蛋黄高密度脂蛋白结构、组成、来源及功能研究进展[J].中国家禽,2016,38(4):38-43.
[3]MOHAMMED S M,MORRISON S,WIMS L,et al.Deposition of genetically engineered human antibodies into the egg yolk of hens[J].Immunotechnology,1998,4(2):115-125.
[4]王丽英.鸡卵黄免疫球蛋白的分离纯化、修饰及其稳定性研究[D].武汉:华中农业大学,2012.
[5]LACA A,PAREDES B,RENDUELES M,et al.Egg yolk granules:Separation,characteristics and applications in food industry[J].LWT-Food Science and Technology,2014,59(1):1-5.
[6]STRIXNER T,STERR J,KULOZIK U,et al.Structural study on hen-egg yolk high density lipoprotein(HDL)granules[J].Food Biophysics,2014,9(4):314-321.
[7]ANTON M.Egg yolk:Structures,functionalities and processes[J].J Sci Food Agric,2013,93(12):2871-2880.
[8]崔焕忠,姜海龙,张加力,等.抗猪传染性胃肠炎病毒与猪流行性腹泻病毒卵黄抗体制备及其临床应用研究[J].中国畜牧兽医,2012,39(6):173-175.
[9]方热军,汤小朋,王清平.卵黄抗体替代抗生素的作用机制及在养猪生产中的应用[J].饲料工业,2016,37(1):1-6.
[10]XU J J,REN C Z,WANG S S,et al.Protection Efficacy of multivalent egg yolk immunoglobulin against eimeria tenella infection in chickens[J].Iranian Journal of Parasitology,2013,8(3):449-458.
[11]贺时飞,张颖,贺建军.抗轮状病毒鸡卵黄免疫球蛋白治疗小儿轮状病毒肠炎临床观察[J].儿科药学杂志,2016,22(6):26-29.
[12]吴凯,徐明星,杨燕,等.抗日本血吸虫可溶性虫卵抗原IgY的制备及初步应用[J].中国血吸虫病防治杂志,2016,28(2):196-198.
[13]王云芸,戴迎春,聂军,等.鸡卵黄抗体用于诺如病毒检测的初步研究[J].热带医学杂志,2013,13(4):433-436.
[14]李晓楠,何金鑫,胡建军,等.基于IgY的间接竞争EILSA检测庆大霉素残留方法的建立[J].中国畜牧兽医学会兽医药理毒理学分会会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会,2015.
[15]薛慧君,孙润广,王小梅,等.卵黄低密度脂蛋白结构的红外光谱和激光拉曼光谱分析[J].光谱学与光谱分析,2010,30(11):2998-3001.
[16]ANTON M,MARTINET V,DALGALARRONDO M,et al.Chemical and structural characterisation of low-density lipoproteins purified from hen egg yolk[J].Food Chemistry,2003,83(2):175-183.
[17]DAUPHAS S,BEAUMAL V,RIAUBLANC A,et al.Hen egg yolk low-density lipoproteins film spreading at the air-water and oil-water interfaces[J].Journal of Agricultural&Food Chemistry,2006,54(10):3733-3737.
[18]LACA A,PAREDES B,RENDUELES M,et al.Egg yolk plasma:Separation,characteristics and future prospects[J].LWT-Food Science and Technology,2015,62(1):7-10.
[19]ANDERSON T A,LEVITT D G,BANASZAK L J.The structural basis of lipid interactions in lipovitellin,a soluble lipoprotein[J].Structure,1998,6(7):895-909.
[20]BANASZAK L J,TIMMIS P,POLIKS B J.The crystal structure of a lipoprotein:Lipovitellin.In:Molecular biology of atherosclerosis[J].Proceedings of the 20th Steenbock Symposium,University of Wisconsin,1990:135-140.
[21]GROCHE D,RASHKOVETSKY L,FALCHUK K,et al.Subunit composition of the zinc proteins alpha-and betalipovitellin from chicken[J].Journal of Protein Chemistry,2000,19(5):379-387.
[22]KASSAIFY Z G,E W L,MINE Y.Identification of antiadhesive fraction(s)in nonimmunized egg yolk powder:in vitro study[J].Journal of Agricultural&Food Chemistry,2005,53(11):4607-4614.
[23]YAMAMOTO Y,OMORI M.Antioxidative activity of egg yolk lipoproteins[J].Bioscience Biotechnology and Biochemistry,1994,58(9):1711-1713.
[24]张晓维.卵黄高磷蛋白的分离纯化、结构表征及功能特性研究[D].武汉:华中农业大学,2014.
[25]陈海英.鸡蛋卵黄高磷蛋白乳化特性研究[D].无锡:江南大学,2013.
[26]CASTELLANI O,BELHOMME C,DAVID-BRIANDE,et al.The role of metal ions in emulsion characteristics and flocculation behaviour of phosvitin-stabilised emulsions[J].Food Hydrocolloids,2008,22(7):1243-1253.
[27]CHUNG S,FERRIER L.Heat denaturation and emulsifying properties of egg yolk phosvitin[J].Journal of Food Science,1995,60(5):906-908.
[28]SAMARAWEERA H,ZHANG W G,LEE E J,et al.Egg yolk phosvitin and functional phosphopeptides-review[J].Journal of Food Science,2011,76(7):143-150.
[29]徐彩娜,殷涌光,王二雷,等.卵黄高磷蛋白及其磷酸肽稳定性和抗氧化性[J].吉林大学学报(工学版),2013,43(2):544-549.
[30]王丽英,马美湖,蔡朝霞,等.离子交换色谱法分离纯化鸡卵黄免疫球蛋白[J].色谱,2012,30(1):80-85.
[31]WANG N,XU Q,LIU Y,et al.Highly efficient extraction and purification of low-density lipoprotein from hen egg yolk[J].Poult Sci,2018,97(6):2230-2238.
[32]ZHANG X,QIU N,GENG F,et al.Simply and effectively preparing high-purity phosvitin using polyethylene glycol and anion-exchange chromatography[J].Journal of Separation Science,2015,34(22):3295-3301.
[2]于智慧,王宁,蔡朝霞,等.鸡蛋蛋黄高密度脂蛋白结构、组成、来源及功能研究进展[J].中国家禽,2016,38(4):38-43.
[3]MOHAMMED S M,MORRISON S,WIMS L,et al.Deposition of genetically engineered human antibodies into the egg yolk of hens[J].Immunotechnology,1998,4(2):115-125.
[4]王丽英.鸡卵黄免疫球蛋白的分离纯化、修饰及其稳定性研究[D].武汉:华中农业大学,2012.
[5]LACA A,PAREDES B,RENDUELES M,et al.Egg yolk granules:Separation,characteristics and applications in food industry[J].LWT-Food Science and Technology,2014,59(1):1-5.
[6]STRIXNER T,STERR J,KULOZIK U,et al.Structural study on hen-egg yolk high density lipoprotein(HDL)granules[J].Food Biophysics,2014,9(4):314-321.
[7]ANTON M.Egg yolk:Structures,functionalities and processes[J].J Sci Food Agric,2013,93(12):2871-2880.
[8]崔焕忠,姜海龙,张加力,等.抗猪传染性胃肠炎病毒与猪流行性腹泻病毒卵黄抗体制备及其临床应用研究[J].中国畜牧兽医,2012,39(6):173-175.
[9]方热军,汤小朋,王清平.卵黄抗体替代抗生素的作用机制及在养猪生产中的应用[J].饲料工业,2016,37(1):1-6.
[10]XU J J,REN C Z,WANG S S,et al.Protection Efficacy of multivalent egg yolk immunoglobulin against eimeria tenella infection in chickens[J].Iranian Journal of Parasitology,2013,8(3):449-458.
[11]贺时飞,张颖,贺建军.抗轮状病毒鸡卵黄免疫球蛋白治疗小儿轮状病毒肠炎临床观察[J].儿科药学杂志,2016,22(6):26-29.
[12]吴凯,徐明星,杨燕,等.抗日本血吸虫可溶性虫卵抗原IgY的制备及初步应用[J].中国血吸虫病防治杂志,2016,28(2):196-198.
[13]王云芸,戴迎春,聂军,等.鸡卵黄抗体用于诺如病毒检测的初步研究[J].热带医学杂志,2013,13(4):433-436.
[14]李晓楠,何金鑫,胡建军,等.基于IgY的间接竞争EILSA检测庆大霉素残留方法的建立[J].中国畜牧兽医学会兽医药理毒理学分会会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会,2015.
[15]薛慧君,孙润广,王小梅,等.卵黄低密度脂蛋白结构的红外光谱和激光拉曼光谱分析[J].光谱学与光谱分析,2010,30(11):2998-3001.
[16]ANTON M,MARTINET V,DALGALARRONDO M,et al.Chemical and structural characterisation of low-density lipoproteins purified from hen egg yolk[J].Food Chemistry,2003,83(2):175-183.
[17]DAUPHAS S,BEAUMAL V,RIAUBLANC A,et al.Hen egg yolk low-density lipoproteins film spreading at the air-water and oil-water interfaces[J].Journal of Agricultural&Food Chemistry,2006,54(10):3733-3737.
[18]LACA A,PAREDES B,RENDUELES M,et al.Egg yolk plasma:Separation,characteristics and future prospects[J].LWT-Food Science and Technology,2015,62(1):7-10.
[19]ANDERSON T A,LEVITT D G,BANASZAK L J.The structural basis of lipid interactions in lipovitellin,a soluble lipoprotein[J].Structure,1998,6(7):895-909.
[20]BANASZAK L J,TIMMIS P,POLIKS B J.The crystal structure of a lipoprotein:Lipovitellin.In:Molecular biology of atherosclerosis[J].Proceedings of the 20th Steenbock Symposium,University of Wisconsin,1990:135-140.
[21]GROCHE D,RASHKOVETSKY L,FALCHUK K,et al.Subunit composition of the zinc proteins alpha-and betalipovitellin from chicken[J].Journal of Protein Chemistry,2000,19(5):379-387.
[22]KASSAIFY Z G,E W L,MINE Y.Identification of antiadhesive fraction(s)in nonimmunized egg yolk powder:in vitro study[J].Journal of Agricultural&Food Chemistry,2005,53(11):4607-4614.
[23]YAMAMOTO Y,OMORI M.Antioxidative activity of egg yolk lipoproteins[J].Bioscience Biotechnology and Biochemistry,1994,58(9):1711-1713.
[24]张晓维.卵黄高磷蛋白的分离纯化、结构表征及功能特性研究[D].武汉:华中农业大学,2014.
[25]陈海英.鸡蛋卵黄高磷蛋白乳化特性研究[D].无锡:江南大学,2013.
[26]CASTELLANI O,BELHOMME C,DAVID-BRIANDE,et al.The role of metal ions in emulsion characteristics and flocculation behaviour of phosvitin-stabilised emulsions[J].Food Hydrocolloids,2008,22(7):1243-1253.
[27]CHUNG S,FERRIER L.Heat denaturation and emulsifying properties of egg yolk phosvitin[J].Journal of Food Science,1995,60(5):906-908.
[28]SAMARAWEERA H,ZHANG W G,LEE E J,et al.Egg yolk phosvitin and functional phosphopeptides-review[J].Journal of Food Science,2011,76(7):143-150.
[29]徐彩娜,殷涌光,王二雷,等.卵黄高磷蛋白及其磷酸肽稳定性和抗氧化性[J].吉林大学学报(工学版),2013,43(2):544-549.
[30]王丽英,马美湖,蔡朝霞,等.离子交换色谱法分离纯化鸡卵黄免疫球蛋白[J].色谱,2012,30(1):80-85.
[31]WANG N,XU Q,LIU Y,et al.Highly efficient extraction and purification of low-density lipoprotein from hen egg yolk[J].Poult Sci,2018,97(6):2230-2238.
[32]ZHANG X,QIU N,GENG F,et al.Simply and effectively preparing high-purity phosvitin using polyethylene glycol and anion-exchange chromatography[J].Journal of Separation Science,2015,34(22):3295-3301.