核酸等温扩增产物检测方法的研究进展
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摘要
核酸等温扩增技术是二十世纪九十年代以来发展起来的一种新技术,相比于需要温度调制的传统核酸扩增技术,核酸等温扩增技术具有设备简单、操作方便等一系列优点,有实际应用价值。本文对核酸等温扩增产物(扩增子)的检测方法作一综述,并对今后的应用研究方向进行了初步探讨。
Isothermal nucleic acid amplification is a new technique developed since 1990's. Compared with traditional nucleic acid amplification techniques which need temperature modulation,isothermal nucleic acid amplification technology has a series of advantages such as simple equipment and convenient operation. It also has practical application value. The major detection methods for isothermal nucleic acid amplification amplicons are reviewed in this paper. Meanwhile, the future research direction of application is preliminarily discussed.
Isothermal nucleic acid amplification is a new technique developed since 1990's. Compared with traditional nucleic acid amplification techniques which need temperature modulation,isothermal nucleic acid amplification technology has a series of advantages such as simple equipment and convenient operation. It also has practical application value. The major detection methods for isothermal nucleic acid amplification amplicons are reviewed in this paper. Meanwhile, the future research direction of application is preliminarily discussed.
引文
[1]王玉倩,薛秀花.实时荧光定量PCR技术研究进展及其应用[J].生物学通报,2016,51:1-6.
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[3] T.Notomi,H. Okayama,H. Masubuchi,et al. Loopmediated isothermal amplification of DNA[J]. Nucleic Acid Research,2000,28:e63.
[4] GL Xu, L. Hu, HY Zhong, et al. Cross priming amplification:mechanism and optimization for isothermal DNA amplification[J]. Scientific Report,2012,2:246-252.
[5] PM Lizardi, XH Huang,ZR Zhu,et al. Mutation detection and single molecule counting using isothermalrolling circle amplification[J]. Nature Genetics,1998,19:225-232.
[6] O Piepenburg,CH Williams,DL Stemple,et al. DNA detection using recombination proteins[J]. Plos Biology,2006,4:1115-1121.
[7] V. Myriam,Y. Xu, HM Kong. Helicase-dependent isothermal DNA amplification[J]. EMBO Reports,2004,5:795-800.
[8]山东出入境检验检疫局检验检疫技术中心.金黄色葡萄球菌的切刻内切酶核酸恒温扩增快速检测试剂盒[P].中国,CN102424836A,2012-04-25.
[9] Walker GT,Little MC,Nadeau JG,et al. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system[M]. Proc Natl Acad Sci USA,1992,89:392-396.
[10] J Compton. Nucleic acid sequence-based amplification[J]. Nature,2005,350:91-92.
[11] CS Hill. Molecular diagnostic testing for infectious diseases using TMA technology[J]. Expert Review of Molecular Diagnostics,2001,1:445-455.
[12] Y.Mori,K. Nagamine,N. Tomita,et al. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation[J]. Biochemical and Biophysical Research Communications,2001,289:150-154.
[13] T. Norihiro,M. Yasuyoshi,K. Hidetoshi,et al. Loopmediated isothermal amplification(LAMP)of gene sequences and simple visual detection of products[J].Nature Protocols,2008,3:877-882.
[14] C. Wang,R. Li,S. Quan,et al. GMO detection in food and feed through screening by visual loop–mediated isothermal amplification assays[J]. Analytical and Bioanalytic Chemistry,2015,407:4829-4834.
[15] D.G. Zhou,J. L. Guo,L. P. Xu,et al. Establishment and application of a loop-mediated isothermal amplification(LAMP)system for detection of cry1Ac transgenic sugarcane[J]. Scientific Reports,2014,4:4912-4919.
[16] K. Karthik,R. Rathore,P. Thomas,et al. New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination[J].MethodsX,2014,1:137-143.
[17] A.Martin,K.Grant,F.Stressmann,et al. Ultimate single-Copy DNA detection using real-time electrochemical LAMP[J]. ACS Sensors,2016,1:904-912.
[18] M. U. Ahmed, M. Saito, M. MHossain, et al.Electrochemical genosensor for the rapid detection of GMO using loop-mediated isothermal amplification[J].Analyst,2009,134:966-972.
[19] M. Ahmed,S. Nahar,M. Safavieh,et al. Real-time electrochemical detection of pathogen DNA using electrostatic interaction of a redox probe[J]. Analyst,2013,138:907-915.
[20] M. Safavieh, M. U. Ahmed, A. Ng, et al. Highthroughput real-time electrochemical monitoring of LAMP for pathogenic bacteria detection[J]. Biosensors and Bioelectronics,2014,58:101-106.
[21] T. Defever,M. Druet,D. Evrard,et al. Real-Time electrochemical PCR with a DNA intercalating redox probe[J]. Analytical Chemistry,2011,83:1815-1821.
[22] C. Toumazou, L. M. Shepherd, S. C. Reed, et al.Simultaneous DNA amplification and detection using a p H-sensing semiconductor system[J]. Nature Methods,2013,10:641-647.
[23] F. Zhang,J. Wu,R. Wang,et al. Portable p Hinspired electrochemical detection of DNA amplification[J]. Chemical Communications,2014,50:8416-8419.
[2] YX. Zhao, F. Chen, Q. Li, et al. Isothermal amplification of nucleic acids[J]. Chemical Reviews,2015,115:12491-12545.
[3] T.Notomi,H. Okayama,H. Masubuchi,et al. Loopmediated isothermal amplification of DNA[J]. Nucleic Acid Research,2000,28:e63.
[4] GL Xu, L. Hu, HY Zhong, et al. Cross priming amplification:mechanism and optimization for isothermal DNA amplification[J]. Scientific Report,2012,2:246-252.
[5] PM Lizardi, XH Huang,ZR Zhu,et al. Mutation detection and single molecule counting using isothermalrolling circle amplification[J]. Nature Genetics,1998,19:225-232.
[6] O Piepenburg,CH Williams,DL Stemple,et al. DNA detection using recombination proteins[J]. Plos Biology,2006,4:1115-1121.
[7] V. Myriam,Y. Xu, HM Kong. Helicase-dependent isothermal DNA amplification[J]. EMBO Reports,2004,5:795-800.
[8]山东出入境检验检疫局检验检疫技术中心.金黄色葡萄球菌的切刻内切酶核酸恒温扩增快速检测试剂盒[P].中国,CN102424836A,2012-04-25.
[9] Walker GT,Little MC,Nadeau JG,et al. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system[M]. Proc Natl Acad Sci USA,1992,89:392-396.
[10] J Compton. Nucleic acid sequence-based amplification[J]. Nature,2005,350:91-92.
[11] CS Hill. Molecular diagnostic testing for infectious diseases using TMA technology[J]. Expert Review of Molecular Diagnostics,2001,1:445-455.
[12] Y.Mori,K. Nagamine,N. Tomita,et al. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation[J]. Biochemical and Biophysical Research Communications,2001,289:150-154.
[13] T. Norihiro,M. Yasuyoshi,K. Hidetoshi,et al. Loopmediated isothermal amplification(LAMP)of gene sequences and simple visual detection of products[J].Nature Protocols,2008,3:877-882.
[14] C. Wang,R. Li,S. Quan,et al. GMO detection in food and feed through screening by visual loop–mediated isothermal amplification assays[J]. Analytical and Bioanalytic Chemistry,2015,407:4829-4834.
[15] D.G. Zhou,J. L. Guo,L. P. Xu,et al. Establishment and application of a loop-mediated isothermal amplification(LAMP)system for detection of cry1Ac transgenic sugarcane[J]. Scientific Reports,2014,4:4912-4919.
[16] K. Karthik,R. Rathore,P. Thomas,et al. New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination[J].MethodsX,2014,1:137-143.
[17] A.Martin,K.Grant,F.Stressmann,et al. Ultimate single-Copy DNA detection using real-time electrochemical LAMP[J]. ACS Sensors,2016,1:904-912.
[18] M. U. Ahmed, M. Saito, M. MHossain, et al.Electrochemical genosensor for the rapid detection of GMO using loop-mediated isothermal amplification[J].Analyst,2009,134:966-972.
[19] M. Ahmed,S. Nahar,M. Safavieh,et al. Real-time electrochemical detection of pathogen DNA using electrostatic interaction of a redox probe[J]. Analyst,2013,138:907-915.
[20] M. Safavieh, M. U. Ahmed, A. Ng, et al. Highthroughput real-time electrochemical monitoring of LAMP for pathogenic bacteria detection[J]. Biosensors and Bioelectronics,2014,58:101-106.
[21] T. Defever,M. Druet,D. Evrard,et al. Real-Time electrochemical PCR with a DNA intercalating redox probe[J]. Analytical Chemistry,2011,83:1815-1821.
[22] C. Toumazou, L. M. Shepherd, S. C. Reed, et al.Simultaneous DNA amplification and detection using a p H-sensing semiconductor system[J]. Nature Methods,2013,10:641-647.
[23] F. Zhang,J. Wu,R. Wang,et al. Portable p Hinspired electrochemical detection of DNA amplification[J]. Chemical Communications,2014,50:8416-8419.