高效液相色谱法测定替考拉宁血药浓度
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摘要
目的:建立高效液相色谱(HPLC)法检测人血浆中替考拉宁浓度的方法。方法:采用Waters System-C_(18)色谱柱,以乙腈:0.01mol/L磷酸二氢钠缓冲液(25:75,V/V,H_3PO_4调pH值为2.53)为流动相,柱温40℃,流速1.2 mL/min;进样量20 L;检测波长215nm。结果:本方法得到标准曲线方程为y=0.053x-0.021(R~2=0.998),人血浆中替考拉宁浓度在3.125～100 mg/L范围内具有良好的线性关系。回收率大于92%,精密度和稳定性的RSD均小于8%。结论:本研究建立的替考拉宁血药浓度HPLC测定法专属性好、操作简单、准确度高、重现性好、回收率和灵敏度高,适用于生物样本分析研究,可满足替考拉宁药动学研究的需要。
Objective: To establish a HPLC method to determine teicoplanin in human plasma. Methods:Chromatographic conditions included Waters C_(18) column, acetonitrile-0.01 mol/L NaH_2 _(25:75,V/V,pH adjusted to 2.53 with H_3 PO_4)as mobile phase, 40 ℃ column temperature, 215 nm detective wavelength, and 1.2 mL/min flow rate. Results:This method generated a standard curve of teicoplanin with good linearty in the concentration of 3.125～100 mg/L(R~2= 0.998),the recovery was over 92%, and the precision and the stability were also qualified as their RSD were below 8%. Conclusion:We believe this method is sensitive, simple, accurate, precisely, and stable. It could be used for the determination of teicoplanin in plasma and fulfill the needs of clinical pharmacokinetics for teicoplanin.
Objective: To establish a HPLC method to determine teicoplanin in human plasma. Methods:Chromatographic conditions included Waters C_(18) column, acetonitrile-0.01 mol/L NaH_2 _(25:75,V/V,pH adjusted to 2.53 with H_3 PO_4)as mobile phase, 40 ℃ column temperature, 215 nm detective wavelength, and 1.2 mL/min flow rate. Results:This method generated a standard curve of teicoplanin with good linearty in the concentration of 3.125～100 mg/L(R~2= 0.998),the recovery was over 92%, and the precision and the stability were also qualified as their RSD were below 8%. Conclusion:We believe this method is sensitive, simple, accurate, precisely, and stable. It could be used for the determination of teicoplanin in plasma and fulfill the needs of clinical pharmacokinetics for teicoplanin.
引文
[1]纪淳安.替考拉宁的抗菌活性和临床应用[J].国外医药:抗生素分册, 2001, 22(3):122
[2] Wilson A, Peter R. Clinical pharmacokinetics of teicoplanin[J]. Clin Pharmacokinet, 2000, 39(3):167
[3] Roberts J A, Stove V, De Waele J J, et al. Variability in protein binding of teicoplanin and achievement of therapeutic drug monitoring targets in critically ill patients:lessons from the DALI Study[J].Int J Antimicrob Agents, 2014, 43(5):423
[4]邓晶晶,卫薇.糖肽类抗生素替考拉宁的分析方法研究进展[J].西南军医, 2009,11(5):925
[5]张谊芳,石夏莹,蒋杰.替考拉宁临床应用98例合理性分析[J].中华危重症医学杂志:电子版, 2014, 7(5):361
[6] Matsumoto K, Kanazawa N, Watanabe E, et al. Development of initial loading procedure for teicoplanin in critically ill patients with severe infections[J]. Biol Pharm Bull, 2013, 36(6):1024
[7]祖育娜,张华,周丽娟,等.不同剂量替考拉宁治疗老年重症革兰阳性菌感染患者的治疗药物浓度监测与疗效评估[J].中华医院感染学杂志, 2016, 26(23):109
[8]石晓丽,周丽娟.血药浓度监测指导下的51例替考拉宁用药分析[J].中国药事, 2016, 30(8):832
[9] Yamada T, Kubota T, Nakamura M, et al. Evaluation of teicoplanin concentrations and safety analysis in neonates[J]. Int J Antimicrob Agents, 2014, 44(5):458
[10]中国国家处方集编委会.中国国家处方集:化学药品与生物制品卷[M].北京:人民军医出版社, 2010:475
[11]孙渊,周鹏,杨建苗,等. HPLC法测定替考拉宁血药浓度[J].中国临床药学杂志, 2015, 24(3):173
[12]艾又生,徐楚鸿,陈志祥,等.高效液相色谱法测定替考拉宁的血药浓度[J].中国医院药学杂志, 2005, 25(3):254
[2] Wilson A, Peter R. Clinical pharmacokinetics of teicoplanin[J]. Clin Pharmacokinet, 2000, 39(3):167
[3] Roberts J A, Stove V, De Waele J J, et al. Variability in protein binding of teicoplanin and achievement of therapeutic drug monitoring targets in critically ill patients:lessons from the DALI Study[J].Int J Antimicrob Agents, 2014, 43(5):423
[4]邓晶晶,卫薇.糖肽类抗生素替考拉宁的分析方法研究进展[J].西南军医, 2009,11(5):925
[5]张谊芳,石夏莹,蒋杰.替考拉宁临床应用98例合理性分析[J].中华危重症医学杂志:电子版, 2014, 7(5):361
[6] Matsumoto K, Kanazawa N, Watanabe E, et al. Development of initial loading procedure for teicoplanin in critically ill patients with severe infections[J]. Biol Pharm Bull, 2013, 36(6):1024
[7]祖育娜,张华,周丽娟,等.不同剂量替考拉宁治疗老年重症革兰阳性菌感染患者的治疗药物浓度监测与疗效评估[J].中华医院感染学杂志, 2016, 26(23):109
[8]石晓丽,周丽娟.血药浓度监测指导下的51例替考拉宁用药分析[J].中国药事, 2016, 30(8):832
[9] Yamada T, Kubota T, Nakamura M, et al. Evaluation of teicoplanin concentrations and safety analysis in neonates[J]. Int J Antimicrob Agents, 2014, 44(5):458
[10]中国国家处方集编委会.中国国家处方集:化学药品与生物制品卷[M].北京:人民军医出版社, 2010:475
[11]孙渊,周鹏,杨建苗,等. HPLC法测定替考拉宁血药浓度[J].中国临床药学杂志, 2015, 24(3):173
[12]艾又生,徐楚鸿,陈志祥,等.高效液相色谱法测定替考拉宁的血药浓度[J].中国医院药学杂志, 2005, 25(3):254