补肾通络方对肺纤维化大鼠肺组织病理形态的影响
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摘要
目的观察补肾通络方对肺纤维化大鼠肺组织病理形态的影响。方法采用博来霉素诱导建立实验性肺纤维化大鼠模型,以氢化可的松琥珀酸钠为对照,观察补肾通络方对肺纤维化大鼠肺组织病理形态及血清羟脯氨酸(HYP)、透明质酸(HA)、层粘连蛋白(LN)的影响。结果同正常组比较,模型组大鼠肺组织病理纤维化显著,炎性反应明显,同时伴有血清HYP,HA,LN升高(P<0.05,P<0.01)。同模型组相比,各治疗组肺组织病理形态明显改善,补肾通络高剂量组大鼠血清HYP,HA,LN含量明显下降(P<0.05);补肾通络低剂量组LN含量明显下降(P<0.01),氢化可的松琥铂酸钠对照组HYP、HA、LN含量明显下降(P<0.05,P<0.01)。结论补肾通络方可改善实验性肺纤维化大鼠肺组织病理形态,抑制肺纤维化。
Objective To observe the effect of bushen tongluo formula on the pathological morphology of lung tissue in the rats with pulmonary fibrosis.Methods Bleomycin induction was adopted to establish the experimental model of pulmonary fibrosis of rat.The hydrocortisone sodium succinate was used as the control.The impacts of bushen tongluo formula were observed on the pathological morphology of lung tissue in the rats with pulmonary fibrosis and serum hydroxyproline(HYP),hyaluronic acid(HA) and laminin(LN).Results Compared with the normal group,the pathological fibrosis of lung tissue was significant in the rats,the inflammatory reaction was remarkable and the levels of HYP,HA and LN were increased in serum in the model group(P<0.05,P<0.01).Compared with the model group,the pathological morphology of lung tissue was improved obviously in each treatment group.The levels of HYP,HA and LN were reduced obviously in the high-dose bushen tongluo formula group(P<0.05),LN was reduced obviously in the low-dose group(P<0.01).In the control group with hydrocortisone sodium succinate,HYP,HA and LN were reduced obviously(P<0.05,P<0.01).Conclusion Bushen tongluo formula improves in the pathological morphology of lung tissue and inhibits pulmonary fibrosis in the experimental rat models with pulmonary fibrosis.
Objective To observe the effect of bushen tongluo formula on the pathological morphology of lung tissue in the rats with pulmonary fibrosis.Methods Bleomycin induction was adopted to establish the experimental model of pulmonary fibrosis of rat.The hydrocortisone sodium succinate was used as the control.The impacts of bushen tongluo formula were observed on the pathological morphology of lung tissue in the rats with pulmonary fibrosis and serum hydroxyproline(HYP),hyaluronic acid(HA) and laminin(LN).Results Compared with the normal group,the pathological fibrosis of lung tissue was significant in the rats,the inflammatory reaction was remarkable and the levels of HYP,HA and LN were increased in serum in the model group(P<0.05,P<0.01).Compared with the model group,the pathological morphology of lung tissue was improved obviously in each treatment group.The levels of HYP,HA and LN were reduced obviously in the high-dose bushen tongluo formula group(P<0.05),LN was reduced obviously in the low-dose group(P<0.01).In the control group with hydrocortisone sodium succinate,HYP,HA and LN were reduced obviously(P<0.05,P<0.01).Conclusion Bushen tongluo formula improves in the pathological morphology of lung tissue and inhibits pulmonary fibrosis in the experimental rat models with pulmonary fibrosis.
引文
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[10]肖洁,张惠丽,徐米清,等.冬虫夏草菌粉对梗阻性肾病大鼠肾组织羟脯氨酸含量及p21表达的影响[J].中华生物医学工程杂志,2015,21(4):156-159.
[11]苏奕亮,翁东,周瑛,等.血清LN、1VC、PⅢNP、HA与特发性肺纤维化严重度和预后的相关性[J].中华急诊医学杂志,2018,27(2):188-193.
[12]宋占帅,张娟,张蓉,等.补阳还五汤联合汉防己甲素片改善矽肺肺纤维化效果观察[J].山东医药,2017,57(42):51-53.
[13]柳湘洁,姚弘毅,涂艳,等.呼吸康复训练联合百令胶囊对矽肺合并COPD患者炎症反应及肺纤维化的影响[J].海南医学院学报,2018,24(4):480-483.
[14]苏韫,张毅,李娟,等.红芪有效部位对肺间质纤维化模型大鼠肺组织胶原面积、透明质酸及层粘连蛋白的影响[J].中国中医药信息杂志,2016,23(4):72-76.
[2]黄成亮,李艳艳,范贤明,等. IL-17A促进博来霉素诱导的肺纤维化大鼠肺组织的炎症形成[J].细胞与分子免疫学杂志,2014,30(4):366-370.
[3]刘晓艳,苏凤哲,刘真.补肾通络方治疗老年特发性肺纤维化的临床体会[J].环球中医药,2017,10(9):9.
[4]苏凤哲.治疗老年肺纤维化关键在于补肾[J].中医临床研究,2017,9(3):3.
[5]张立山,戴雁彦.武维屏教授治疗肺纤维化十法[J].中国中医药信息杂志,2008,15(4):94-95.
[6]郭素芳.中医药疗法在特发性肺纤维化治疗中的应用[J].中国中医药信息杂志,2008,15(2):90-91.
[7]樊茂荣,苗青.特发性肺间质纤维化的中医治疗思路[J].陕西中医,2010,31(10):1070-1071.
[8]李辉,李国勤.益气活血通络法治疗特发性肺纤维化气虚血瘀证疗效观察[J].中国中医急症,2008,17(10):1341-1342.
[9]苏凤哲,赵昕,周梦佳.补肾通络治疗老年肺纤维化临床研究[J].中医临床研究,2014,6(3):107.
[10]肖洁,张惠丽,徐米清,等.冬虫夏草菌粉对梗阻性肾病大鼠肾组织羟脯氨酸含量及p21表达的影响[J].中华生物医学工程杂志,2015,21(4):156-159.
[11]苏奕亮,翁东,周瑛,等.血清LN、1VC、PⅢNP、HA与特发性肺纤维化严重度和预后的相关性[J].中华急诊医学杂志,2018,27(2):188-193.
[12]宋占帅,张娟,张蓉,等.补阳还五汤联合汉防己甲素片改善矽肺肺纤维化效果观察[J].山东医药,2017,57(42):51-53.
[13]柳湘洁,姚弘毅,涂艳,等.呼吸康复训练联合百令胶囊对矽肺合并COPD患者炎症反应及肺纤维化的影响[J].海南医学院学报,2018,24(4):480-483.
[14]苏韫,张毅,李娟,等.红芪有效部位对肺间质纤维化模型大鼠肺组织胶原面积、透明质酸及层粘连蛋白的影响[J].中国中医药信息杂志,2016,23(4):72-76.