香菊感冒颗粒挥发油指纹图谱及其药材归属研究
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摘要
目的:建立香菊感冒颗粒挥发油的指纹谱图,且对指纹图谱中的特征峰进行药材归属。方法:采用气相色谱质谱联用技术(GC-MS)建立了香菊感冒颗粒挥发油的指纹图谱,并且使用单味药和阴性药材对指纹图谱的特征峰进行了药材归属。结果:香菊感冒颗粒对照指纹图谱中共有22个特征峰,18批香菊感冒颗粒的相似度在0.892~0.998之间;22个特征峰中有19个峰确定了药材归属,有22个化学组分被鉴定。结论:该方法准确可靠,重复性好,可用于香菊感冒颗粒的质量控制。
Objective: To establish the fingerprint of the volatile oil in Xiangjuganmao granules and study the attribution of original herbs. Methods: The fingerprint of the volatile oil was established by GC-MS,and the characteristic peaks were used to study the attribution of original herbs by comparing with those of single herb and negative herb. Results: Totally 22 characteristic peaks were shown in the fingerprint of Xiangjuganmao granules. The similarities of 18 batches of Xiangjuganmao granules were within the range of 0. 892-0. 998. Among the 22 peaks,19 ones were defined the attribution of original herbs,and 22 chemical components were identified. Conclusion: The method is with good precision,reproducibility and stability,which can be used to control the quality of Xiangjuganmao granules.
Objective: To establish the fingerprint of the volatile oil in Xiangjuganmao granules and study the attribution of original herbs. Methods: The fingerprint of the volatile oil was established by GC-MS,and the characteristic peaks were used to study the attribution of original herbs by comparing with those of single herb and negative herb. Results: Totally 22 characteristic peaks were shown in the fingerprint of Xiangjuganmao granules. The similarities of 18 batches of Xiangjuganmao granules were within the range of 0. 892-0. 998. Among the 22 peaks,19 ones were defined the attribution of original herbs,and 22 chemical components were identified. Conclusion: The method is with good precision,reproducibility and stability,which can be used to control the quality of Xiangjuganmao granules.
引文
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13 李佳,刘红燕,张永清.顶空固相微萃取-气质色谱联用技术分析海州香薷与石香薷中挥发性成分[J].中国实验方剂学杂志,2013,19(16):118-122
14 中国药典[S].2015年版.一部.397
2 刘红淼,李艳玲,杨继章.广藿香油的药理作用研究进展[J].中国药房,2012,23(47):4506-4508
3 黄罗生,顾燕飞,李红.中药挥发油及芳香性药物的研究进展[J].中国中药杂志,2009,55(12):1606-1611
4 刘世增,杨吉成,顾振纶.香菊感冒冲剂的药理作用研究[J].中成药,1997,19(3):25-27
5 闭兴明,孟日增,郭娜,等.麝香草酚体外抗真菌活性研究[J].中国农学通报,2009,25(1):21-24
6 孙宇,庄严.中药挥发油抗肿瘤研究进展[J].黑龙江科技信息,2015,8:23-23
7 丁长玲,崔俊凤,张雪美,等.复方三黄酊HPLC指纹图谱的建立和化学成分的归属[J].中国药师,2017,20(9):1564-1566,1572
8 中国药典[S].2015年版.四部.203
9 别文华,邓蔚,李力,等.金玄痔科熏洗散有效部位制剂的GC-MS指纹图谱研究[J].中国药师,2015,18(3):390-393
10 佘金明,董红霞,梁逸曾,等.青蒿挥发油成分的GC-MS分析与化学计量学解析法[J].中成药,2011,33(1):99-103
11 宋青坡,韩永成,陈宁,等.不同产地野菊花挥发油成分气相指纹图谱研究[J].中国实验方剂学杂志,2014,20(11):79-82
12 杨银慧,豆小文,孔维军,等.GC-MS法对市售广藿香油的成分分析及质量评价[J].中南药学,2015,13(6):631-634
13 李佳,刘红燕,张永清.顶空固相微萃取-气质色谱联用技术分析海州香薷与石香薷中挥发性成分[J].中国实验方剂学杂志,2013,19(16):118-122
14 中国药典[S].2015年版.一部.397