摘要
目的建立淫羊藿中黄酮类成分HPLC指纹图谱。方法采用Agilent ZORBAX Eclispse SB-C18色谱柱(4.6 mm×150 mm,5μm),以乙腈(A)-水(B)为流动相,梯度洗脱(0~8 min,15%→25%A;8~25 min,25%A;25~32 min,25%→33%A,32~48 min,33%→38%A;48~60 min,38%→60%A;60~70 min,60%→85%A;70~75min,15%A),流速1.0m L/min,柱温30℃,检测波长为270nm,进样量10μL。对11批淫羊藿指纹图谱进行相似度分析及方法学考察。结果 11批淫羊藿药材有21个共有峰,相似度为0.845~0.952,对5个色谱峰进行了归属;方法学考察结果表明,建立的分析方法重复性、精密度、稳定性良好;相似度分析将11批淫羊藿分为4大类,与淫羊藿物种鉴定结果一致。结论不同产地淫羊藿有一定的相似性,HPLC指纹图谱能够为淫羊藿的物种鉴定及内在质量评价提供依据。
Objective To establish HPLC fingerprints of flavonoids ingredients in Epimedii Folium. Methods The chromatographic fingerprint was obtained with Agilent ZORBAX Eclispse SB-C18(4.6 mm × 150 mm, 5 μm) as chromatographic column, with acetonitrile(A)-water(B) as the mobile phase for gradient elution(0–8 min, 15%→25% A; 8–25 min, 25% A; 25–32 min, 25%→33% A; 32–48 min, 33%→38% A; 48–60 min, 38%→60% A; 60–70 min, 60%→85% A; 70~75 min, 15% A). The flow rate was 1.0 mL/min, and the column temperature was maintained at 30 ℃. The detection wavelength was set at 270 nm. The similarity analysis and methodological investigation were conducted to fingerprints of 11 batches of Epimedii Folium. Results There were 21 common peaks with similarities between 0.845 and 0.952 in 11 batches of Epimedii Folium and 5 chromatographic peaks were identified. The methodological investigation results showed that the established analytical method had good repeatability, precision and stability. Similarity analysis divided 11 batches of Epimedii Folium into 4 categories, consistent with the identification results of Epimedii Folium species. Conclusion Epimedii Folium from different producing areas have a certain of similarities. HPLC fingerprints can provide basis for species identification and intrinsic quality evaluation of Epimedii Folium.
引文
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