淫羊藿黄酮类成分指纹图谱研究
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摘要
目的建立淫羊藿中黄酮类成分HPLC指纹图谱。方法采用Agilent ZORBAX Eclispse SB-C18色谱柱(4.6 mm×150 mm,5μm),以乙腈(A)-水(B)为流动相,梯度洗脱(0～8 min,15%→25%A;8～25 min,25%A;25～32 min,25%→33%A,32～48 min,33%→38%A;48～60 min,38%→60%A;60～70 min,60%→85%A;70～75min,15%A),流速1.0m L/min,柱温30℃,检测波长为270nm,进样量10μL。对11批淫羊藿指纹图谱进行相似度分析及方法学考察。结果 11批淫羊藿药材有21个共有峰,相似度为0.845～0.952,对5个色谱峰进行了归属;方法学考察结果表明,建立的分析方法重复性、精密度、稳定性良好;相似度分析将11批淫羊藿分为4大类,与淫羊藿物种鉴定结果一致。结论不同产地淫羊藿有一定的相似性,HPLC指纹图谱能够为淫羊藿的物种鉴定及内在质量评价提供依据。
Objective To establish HPLC fingerprints of flavonoids ingredients in Epimedii Folium. Methods The chromatographic fingerprint was obtained with Agilent ZORBAX Eclispse SB-C18(4.6 mm × 150 mm, 5 μm) as chromatographic column, with acetonitrile(A)-water(B) as the mobile phase for gradient elution(0–8 min, 15%→25% A; 8–25 min, 25% A; 25–32 min, 25%→33% A; 32–48 min, 33%→38% A; 48–60 min, 38%→60% A; 60–70 min, 60%→85% A; 70～75 min, 15% A). The flow rate was 1.0 mL/min, and the column temperature was maintained at 30 ℃. The detection wavelength was set at 270 nm. The similarity analysis and methodological investigation were conducted to fingerprints of 11 batches of Epimedii Folium. Results There were 21 common peaks with similarities between 0.845 and 0.952 in 11 batches of Epimedii Folium and 5 chromatographic peaks were identified. The methodological investigation results showed that the established analytical method had good repeatability, precision and stability. Similarity analysis divided 11 batches of Epimedii Folium into 4 categories, consistent with the identification results of Epimedii Folium species. Conclusion Epimedii Folium from different producing areas have a certain of similarities. HPLC fingerprints can provide basis for species identification and intrinsic quality evaluation of Epimedii Folium.
Objective To establish HPLC fingerprints of flavonoids ingredients in Epimedii Folium. Methods The chromatographic fingerprint was obtained with Agilent ZORBAX Eclispse SB-C18(4.6 mm × 150 mm, 5 μm) as chromatographic column, with acetonitrile(A)-water(B) as the mobile phase for gradient elution(0–8 min, 15%→25% A; 8–25 min, 25% A; 25–32 min, 25%→33% A; 32–48 min, 33%→38% A; 48–60 min, 38%→60% A; 60–70 min, 60%→85% A; 70～75 min, 15% A). The flow rate was 1.0 mL/min, and the column temperature was maintained at 30 ℃. The detection wavelength was set at 270 nm. The similarity analysis and methodological investigation were conducted to fingerprints of 11 batches of Epimedii Folium. Results There were 21 common peaks with similarities between 0.845 and 0.952 in 11 batches of Epimedii Folium and 5 chromatographic peaks were identified. The methodological investigation results showed that the established analytical method had good repeatability, precision and stability. Similarity analysis divided 11 batches of Epimedii Folium into 4 categories, consistent with the identification results of Epimedii Folium species. Conclusion Epimedii Folium from different producing areas have a certain of similarities. HPLC fingerprints can provide basis for species identification and intrinsic quality evaluation of Epimedii Folium.
引文
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[6]蒋军富,李雄英,吴耀生,等.绞股蓝的RAPD指纹图谱鉴定及其特异DNA片段克隆-序列分析[J].中药材,2009,32(2):190-193.
[7]聂韡,朱培林,房海灵,等.HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量[J].中草药,2017,48(1):185-191.
[8]黄再强,陈玲,张燕飞,等.川产几种葛根类药材资源品种的UPLC指纹图谱[J].中国实验方剂学杂志,2017,23(7):41-45.
[9]卢凤来,王磊,颜小捷,等.金银花和山银花的1H-NMR指纹图谱鉴别[J].中成药,2017,39(1):222-224.
[10]曾慧婷,沙秀秀,宿树兰,等.不同产地丹参茎叶UPLC指纹图谱与化学模式识别研究[J].中草药,2017,48(4):767-772.
[11]于桂芳,闫显光,殷洪梅,等.益心舒片的HPLC指纹图谱[J].中国实验方剂学杂志,2017,23(1):69-74.
[12]汪露露,何丹丹,王满,等.基于指纹图谱与主成分分析相结合的复方虎杖方提取工艺研究[J].中草药,2017,48(2):278-282.
[13]梁少梅,张鑫,李敏婷,等.清肺愈痤丸HPLC指纹图谱建立及4种成分测定[J].中成药,2017,39(1):90-93.
[14]刘国雄,李华,张军,等.UPLC指纹图谱快速评价银杏叶提取物及制剂的质量[J].中国实验方剂学杂志,2017,23(1):64-68.
[2]徐艳琴,李作洲,张学军,等.三种药用淫羊藿的地理分布于资源调查[J].武汉植物学研究,2008,26(1):91-98.
[3]裴利宽,黄文华,何天谷,等.中药淫羊藿主要资源种类药材质量的系统研究[J].中国中药杂志,2007,32(21):2217-2222.
[4]裴利宽,郭宝林,黄文华.淫羊藿属主要资源种类的HPLC指纹图谱特征和种类鉴定[J].中国中药杂志,2008,33(14):1662-1668.
[5]李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3104.
[6]蒋军富,李雄英,吴耀生,等.绞股蓝的RAPD指纹图谱鉴定及其特异DNA片段克隆-序列分析[J].中药材,2009,32(2):190-193.
[7]聂韡,朱培林,房海灵,等.HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量[J].中草药,2017,48(1):185-191.
[8]黄再强,陈玲,张燕飞,等.川产几种葛根类药材资源品种的UPLC指纹图谱[J].中国实验方剂学杂志,2017,23(7):41-45.
[9]卢凤来,王磊,颜小捷,等.金银花和山银花的1H-NMR指纹图谱鉴别[J].中成药,2017,39(1):222-224.
[10]曾慧婷,沙秀秀,宿树兰,等.不同产地丹参茎叶UPLC指纹图谱与化学模式识别研究[J].中草药,2017,48(4):767-772.
[11]于桂芳,闫显光,殷洪梅,等.益心舒片的HPLC指纹图谱[J].中国实验方剂学杂志,2017,23(1):69-74.
[12]汪露露,何丹丹,王满,等.基于指纹图谱与主成分分析相结合的复方虎杖方提取工艺研究[J].中草药,2017,48(2):278-282.
[13]梁少梅,张鑫,李敏婷,等.清肺愈痤丸HPLC指纹图谱建立及4种成分测定[J].中成药,2017,39(1):90-93.
[14]刘国雄,李华,张军,等.UPLC指纹图谱快速评价银杏叶提取物及制剂的质量[J].中国实验方剂学杂志,2017,23(1):64-68.