绿萼梅药材超高效液相色谱指纹图谱研究
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摘要
目的采用超高效液相色谱法(ultra performance liquid chromatography,UPLC)建立绿萼梅药材的指纹图谱,为有效控制绿萼梅药材的质量奠定基础。方法采用CORTECS C18色谱柱(2.1mm×100mm,1.6μm),以乙腈(A)-0.1%甲酸水(B)溶液为流动相进行梯度洗脱,进样量为1μL,流速为0.3mL/min,检测波长260nm。结果通过相似度分析确定19个色谱峰构成绿萼梅药材指纹图谱的特征峰。结论采用UPLC方法建立的指纹图谱具有精密度、稳定性、重复性良好的特点,可为绿萼梅化学成分的进一步提取分离和药效物质基础的研究提供科学依据。
Objective To establish the fingerprints of Mume Flos by ultra-performance liquid chromatography(UPLC),and to provide a basis for the effective control of quality of Mume Flos.Methods UPLC was performed on a CORTECS C18 column(2.1 mm ×100 mm,1.6 mm)with a mobile phase of acetonitrile(A)-0.1%formic acid(B)solution for gradient elution at a sample size was of 1μL,a flow rate of0.3 mL/min,and a detection wavelength of 260 nm.Results Similarity analysis identified 19 characteristic peaks in the fingerprints of Mume Flos.Conclusion The fingerprints established using UPLC are accurate,stable,and reproducible,and provide a scientific basis for further isolation of chemical constituents from Mume Flos and basic research on its effective components.
Objective To establish the fingerprints of Mume Flos by ultra-performance liquid chromatography(UPLC),and to provide a basis for the effective control of quality of Mume Flos.Methods UPLC was performed on a CORTECS C18 column(2.1 mm ×100 mm,1.6 mm)with a mobile phase of acetonitrile(A)-0.1%formic acid(B)solution for gradient elution at a sample size was of 1μL,a flow rate of0.3 mL/min,and a detection wavelength of 260 nm.Results Similarity analysis identified 19 characteristic peaks in the fingerprints of Mume Flos.Conclusion The fingerprints established using UPLC are accurate,stable,and reproducible,and provide a scientific basis for further isolation of chemical constituents from Mume Flos and basic research on its effective components.
引文
[1]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:311.
[2]王灿灿,张伟,吴德玲,等.白梅花化学成分及其药理作用研究进展[J].广州化工,2017,45(24):40-42.
[3]张清华,张玲,尚立霞,等.白梅花的化学成分研究[J].中药材,2008,3(11):1666-1668.
[4]FUJIMOTO K,NAKAMURA S,MATSUMOTO T,et al.Structures of acylated sucroses from the flower buds of Prunus mume[J].Journal of Natural Medicines,2014,68(3):481-487.
[5]NAKAMURA S,FUJIMOTO K,MATSUMOTO T,et al.Structures of acylated sucroses and an acylated flavonol glycoside and inhibitory effects of constituents on aldose reductase from the flower buds of Prunus mume[J].Journal of Natural Medicines,2013,67(4):799-806.
[6]FUJIMOTO K,NAKAMURA S,MATSUMOTO T,et al.Medicinal flowers.XXXVIII.structures of acylated sucroses and inhibitory effects of constituents on aldose reducatase from the flower buds of Prunus mume[J].Chemical Pharmaceutical Bulletin,2013,61(4):445-451.
[7]张伟,郭晶晶,郑太华,等.不同产地梅花中绿原酸含量测定[J].安徽中医学院学报,2013,32(6):89-90.
[8]张晓霞,房经贵,陈世忠,等.高效液相色谱法测定中药梅花中绿原酸、芦丁、金丝桃苷和异槲皮素的含量[J].中国医院药学杂志,2016,36(1):107-109.
[2]王灿灿,张伟,吴德玲,等.白梅花化学成分及其药理作用研究进展[J].广州化工,2017,45(24):40-42.
[3]张清华,张玲,尚立霞,等.白梅花的化学成分研究[J].中药材,2008,3(11):1666-1668.
[4]FUJIMOTO K,NAKAMURA S,MATSUMOTO T,et al.Structures of acylated sucroses from the flower buds of Prunus mume[J].Journal of Natural Medicines,2014,68(3):481-487.
[5]NAKAMURA S,FUJIMOTO K,MATSUMOTO T,et al.Structures of acylated sucroses and an acylated flavonol glycoside and inhibitory effects of constituents on aldose reductase from the flower buds of Prunus mume[J].Journal of Natural Medicines,2013,67(4):799-806.
[6]FUJIMOTO K,NAKAMURA S,MATSUMOTO T,et al.Medicinal flowers.XXXVIII.structures of acylated sucroses and inhibitory effects of constituents on aldose reducatase from the flower buds of Prunus mume[J].Chemical Pharmaceutical Bulletin,2013,61(4):445-451.
[7]张伟,郭晶晶,郑太华,等.不同产地梅花中绿原酸含量测定[J].安徽中医学院学报,2013,32(6):89-90.
[8]张晓霞,房经贵,陈世忠,等.高效液相色谱法测定中药梅花中绿原酸、芦丁、金丝桃苷和异槲皮素的含量[J].中国医院药学杂志,2016,36(1):107-109.