不同叶型五指毛桃的形态及生理指标测定
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摘要
五指毛桃(Ficus hirta Vahl)具有较高的遗传多样性。本研究对野外不同叶型五指毛桃数量及生长环境展开调查,并以新鲜采摘的五指毛桃为实验材料,通过测定其叶柄长、叶片长、叶片宽、叶面积、叶周长、表皮毛长度、密度、表皮扁平细胞和气孔器等形态学指标及叶绿素、脯氨酸、可溶性糖含量等生理学指标,比较不同叶型五指毛桃间的差异。结果表明:野外4种叶型五指毛桃数量从大到小依次为:全缘型、三指深裂型、浅裂型、五指深裂型;且其在叶片宽、叶周长、叶面积、表皮毛长度和密度、上表皮扁平细胞形态、下表皮气孔密度和气孔宽、根茎叶含水量、脯氨酸、可溶性糖含量等方面均存在差异。本研究将为进一步了解和利用不同叶型五指毛桃种质资源提供参考依据。
Ficus hirta Vahl has high genetic diversity. In this study, the number and growth environment of Ficus hirta Vahl with different leaf types in the field were investigated. The fresh-picked Ficus hirta Vahl were used as experimental materials. The morphological indexes such as petiole length, leaf length, leaf width, leaf area, leaf perimeter, epidermal hair length, density, epidermal flat cells and stomas, and the physiological indexes such as chlorophyll, proline and soluble sugar content were measured and compared among different leaf types of Ficus hirta Vahl. The results showed that the number of Ficus hirta Vahl with four leaf types in the field was in the order of undivided Ficus hirta Vahl, three-finger partite Ficus hirta Vahl, lobed Ficus hirta Vahl and five-finger partite Ficus hirta Vahl, and there were differences in leaf width, leaf perimeter, leaf area, epidermal hair length and density, upper epidermal flat cell morphology, lower epidermal stomatal density and stomatal width, root, stem and leaf water content, proline and soluble sugar content among the four leaf types. The experiment will provide reference for further understanding and utilizing different leaf types of Ficus hirta Vahl germplasm resources.
Ficus hirta Vahl has high genetic diversity. In this study, the number and growth environment of Ficus hirta Vahl with different leaf types in the field were investigated. The fresh-picked Ficus hirta Vahl were used as experimental materials. The morphological indexes such as petiole length, leaf length, leaf width, leaf area, leaf perimeter, epidermal hair length, density, epidermal flat cells and stomas, and the physiological indexes such as chlorophyll, proline and soluble sugar content were measured and compared among different leaf types of Ficus hirta Vahl. The results showed that the number of Ficus hirta Vahl with four leaf types in the field was in the order of undivided Ficus hirta Vahl, three-finger partite Ficus hirta Vahl, lobed Ficus hirta Vahl and five-finger partite Ficus hirta Vahl, and there were differences in leaf width, leaf perimeter, leaf area, epidermal hair length and density, upper epidermal flat cell morphology, lower epidermal stomatal density and stomatal width, root, stem and leaf water content, proline and soluble sugar content among the four leaf types. The experiment will provide reference for further understanding and utilizing different leaf types of Ficus hirta Vahl germplasm resources.
引文
Feng Y.,Ding A.Q.,and Pan D.M.,2016,Content of soluble sugar and expression analysis of NtTIM gene from bud in Narcissus tazetta var.chinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(12):294-301(冯莹,丁安琪,潘东明,2016,水仙芽可溶性糖含量及NtTIM基因的表达分析,基因组学与应用生物学,35(12):294-301)
Hang Y.Y.,Huang C.H.,Mu S.,and Xu L.S.,2004,Study on leaf morphological diversity of Dioscorea zingiberensis,Zhiw u Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),26(4):398-404(杭悦宇,黄春洪,穆森,徐珞珊,2004,盾叶薯蓣叶片形态多样性研究,植物分类与资源学报,26(4):398-404)
Li J.L.,Han L.,Jin M.,Zhang H.C.,and Su Y.H.,2015,Characteristics analysis of Ammopiptanthus mongolicus stomata and inward potassium channels in guard cell of Ammopip-tanthus mongolicus,Jiyinzuxue Yu Yingyong Shengwuxue(Ge nomics and Applied Biology),34(11):2502-2507(李俊林,韩蕾,金曼,张焕朝,苏彦华,2015,蒙古沙冬青气孔及保卫细胞内向钾离子通道特性研究,基因组学与应用生物学,34(11):2502-2507)
Liu Q.,Li F.R.,and Peng W.,2017,Estimating in situ maximum net photosynthetic rate of Larix olgensis based on abscised measurement,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),28(10):3208-3216(刘强,李凤日,彭娓,2017,基于离体测量估计长白落叶松原位最大净光合速率的方法,应用生态学报,28(10):3208-3216)
Liu X.Z.,2010,Extraction and antioxidant activity of active component in Ficus hirta Vahl,Thesis for M.S.,South China Normal University,Supervisor:Peng Y.H.,pp.1-7(刘肖珍,2010,五指毛桃活性成分的提取与功效研究,硕士学位论文,华南师范大学,导师:彭永宏,pp.1-7)
Liu Y.X.,Wang J.,and Lan H.Y.,2017,Advance in gene regulatory network of plant trichome development controlling,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):196-204(刘艳霞,王娟,兰海燕,2017,基因调控网络调节植物表皮毛发育的研究进展,分子植物育种,15(4):196-204)
Ma Y.J.,Liu H.,Shi J.J.,Shang M.Y.,Li Y.L.,Liu G.X.,Xu F.,and Cai S.Q.,2017,Study on quality standard of Fici Hirtae Radi,Zhongcaoyao(Chinese Traditional and Herbal Drugs),48(4):782-791(马雅静,刘焕,史晶晶,尚明英,李耀利,刘广学,徐风,蔡少青,2017,五指毛桃的质量标准研究,中草药,48(4):782-791)
Tian Y.,Jiang J.L.,Shen J.X.,and Li L.,2017,Effect of low temperature stress on growth and physiological indexes of five cultivars of cucumber,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(5):352-358(田雲,蒋景龙,沈季雪,李丽,2017,低温胁迫对5个栽培品种黄瓜生长及生理指标的影响,分子植物育种,15(5):352-358)
Wang Y.,2008,Pharmacological studies on invigorating qi of radix fci hirtae and comparison with astragalus,Thesis for M.S.,Guangzhou University of Chinese Medicine,Supervisor:Ye M.R.,pp.4-6(王艳,2008,五指毛桃益气作用的药理研究及与黄芪的比较,硕士学位论文,广州中医药大学,导师:叶木荣,pp.4-6)
Hang Y.Y.,Huang C.H.,Mu S.,and Xu L.S.,2004,Study on leaf morphological diversity of Dioscorea zingiberensis,Zhiw u Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),26(4):398-404(杭悦宇,黄春洪,穆森,徐珞珊,2004,盾叶薯蓣叶片形态多样性研究,植物分类与资源学报,26(4):398-404)
Li J.L.,Han L.,Jin M.,Zhang H.C.,and Su Y.H.,2015,Characteristics analysis of Ammopiptanthus mongolicus stomata and inward potassium channels in guard cell of Ammopip-tanthus mongolicus,Jiyinzuxue Yu Yingyong Shengwuxue(Ge nomics and Applied Biology),34(11):2502-2507(李俊林,韩蕾,金曼,张焕朝,苏彦华,2015,蒙古沙冬青气孔及保卫细胞内向钾离子通道特性研究,基因组学与应用生物学,34(11):2502-2507)
Liu Q.,Li F.R.,and Peng W.,2017,Estimating in situ maximum net photosynthetic rate of Larix olgensis based on abscised measurement,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),28(10):3208-3216(刘强,李凤日,彭娓,2017,基于离体测量估计长白落叶松原位最大净光合速率的方法,应用生态学报,28(10):3208-3216)
Liu X.Z.,2010,Extraction and antioxidant activity of active component in Ficus hirta Vahl,Thesis for M.S.,South China Normal University,Supervisor:Peng Y.H.,pp.1-7(刘肖珍,2010,五指毛桃活性成分的提取与功效研究,硕士学位论文,华南师范大学,导师:彭永宏,pp.1-7)
Liu Y.X.,Wang J.,and Lan H.Y.,2017,Advance in gene regulatory network of plant trichome development controlling,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):196-204(刘艳霞,王娟,兰海燕,2017,基因调控网络调节植物表皮毛发育的研究进展,分子植物育种,15(4):196-204)
Ma Y.J.,Liu H.,Shi J.J.,Shang M.Y.,Li Y.L.,Liu G.X.,Xu F.,and Cai S.Q.,2017,Study on quality standard of Fici Hirtae Radi,Zhongcaoyao(Chinese Traditional and Herbal Drugs),48(4):782-791(马雅静,刘焕,史晶晶,尚明英,李耀利,刘广学,徐风,蔡少青,2017,五指毛桃的质量标准研究,中草药,48(4):782-791)
Tian Y.,Jiang J.L.,Shen J.X.,and Li L.,2017,Effect of low temperature stress on growth and physiological indexes of five cultivars of cucumber,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(5):352-358(田雲,蒋景龙,沈季雪,李丽,2017,低温胁迫对5个栽培品种黄瓜生长及生理指标的影响,分子植物育种,15(5):352-358)
Wang Y.,2008,Pharmacological studies on invigorating qi of radix fci hirtae and comparison with astragalus,Thesis for M.S.,Guangzhou University of Chinese Medicine,Supervisor:Ye M.R.,pp.4-6(王艳,2008,五指毛桃益气作用的药理研究及与黄芪的比较,硕士学位论文,广州中医药大学,导师:叶木荣,pp.4-6)