男性不同类型染色体多态性对常规体外受精治疗结局的影响
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摘要
目的:探讨男性不同类型染色体多态性对常规体外受精(IVF)治疗结局的影响。方法:回顾性分析2014年6月-2016年6月在本院接受常规IVF行染色体核型分析的患者,根据男性染色体多态性分为4组,其中D/G随体增加者为A组21例,次溢痕增加(包括1号、9号、16号染色体)为B组20例,9号染色体臂间倒位为C组17例,Y染色体变异(包括大Y和小Y)为D组20例,正常者为对照组120例。对各组精液参数、胚胎发育以及临床妊娠结局进行比较。结果:5组精液量、精子浓度、前向精子百分比、存活率和精子正常形态比较差异无统计学意义(P>0.05),正常受精率、总受精率、优胚率、植入率、床妊娠率以及早期流产率差异无统计学意义(P>0.05)。结论:男性染色体不同类型的多态性对于实施常规IVF-ET治疗结局无明显影响。
Objective:To investigate the effects of different types of chromosomal polymorphism of male on outcomes of conventional in vitro fertilization(IVF).Methods:A retrospective analysis was performed about patients undergone routine IVF AND karyotype analysis in the fourth hospital of Hebei medical university between June 2014 to June2016.According to the chromosome polymorphism of male,the patients were divided into four groups:patients with D/G satellite increased in group A(n=21),patients with secondary constriction increases,which included 1,9,and16 chromosomes in group B(n=20),patients with pericentric inversion of chromosome 9 in group C(n=17),patients with big or small Y chromosome in group D(n=20),and patients with normal karyotype in group E(n=120).The semen parameters,situation of embryonic development,and pregnancy outcomes were compared among the groups.Results:There were no statistical significant different in semen volume,sperm concentration,percentage of sperm moving forward,rate of sperm viability,and rate of sperm normal morphology among the five groups(P>0.05).And There were also no statistically significant different in normal fertilization rate,total fertilization rate,good embryo rate,embryo implantation rate,clinical pregnancy rate,and early abortion rate among the five groups(P>0.05).Conclusion:The different types of male chromosome polymorphism has no any significant effect on outcomes of routine IVF.
Objective:To investigate the effects of different types of chromosomal polymorphism of male on outcomes of conventional in vitro fertilization(IVF).Methods:A retrospective analysis was performed about patients undergone routine IVF AND karyotype analysis in the fourth hospital of Hebei medical university between June 2014 to June2016.According to the chromosome polymorphism of male,the patients were divided into four groups:patients with D/G satellite increased in group A(n=21),patients with secondary constriction increases,which included 1,9,and16 chromosomes in group B(n=20),patients with pericentric inversion of chromosome 9 in group C(n=17),patients with big or small Y chromosome in group D(n=20),and patients with normal karyotype in group E(n=120).The semen parameters,situation of embryonic development,and pregnancy outcomes were compared among the groups.Results:There were no statistical significant different in semen volume,sperm concentration,percentage of sperm moving forward,rate of sperm viability,and rate of sperm normal morphology among the five groups(P>0.05).And There were also no statistically significant different in normal fertilization rate,total fertilization rate,good embryo rate,embryo implantation rate,clinical pregnancy rate,and early abortion rate among the five groups(P>0.05).Conclusion:The different types of male chromosome polymorphism has no any significant effect on outcomes of routine IVF.
引文
[1]李丽莎,任洪进,宁郁玲,等.138例染色体多态的细胞遗传学分析及临床表现[J].中华医学遗传学杂志,2007,24(3):362.
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[3] Caglayan A,Ozyazgan I,Demiryilmaz F,et al.Are heterochromatin polymorphisms associated with recurrent miscarriage?[J].Obs Gyn Res,2010,36(4):774-776.
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[8] Conly,Rieder E.The verteb rate cellk inetochore and roles during itosis[J].Trends Cell Biology,1998,8(4):310.
[9]杨丽霞,张琪瑶,孙伟.31例染色体多态性与生殖异常的关系[J].中国优生与遗传杂志,2008,16(4):38-39.
[10]刘波,李山,秦雪,等.异常孕产史夫妇染色体的分析[J].中国优生与遗传杂志,2005,13(4):45-46.
[11]程涌江,叶竞妍.Y染色体长度变异与疾病的关系[J].中国优生与遗传杂志,2005,13(6):34-35.
[12]韩芳,刘娟,翁宁,等.染色体异常对体外胚胎发育的影响[J].中华医学遗传学杂志,2007,24(1):112.
[13]周焕庚,夏家辉,张思仲.人类染色体[M].北京:科学出版社,1987,61-63.
[14]胡晓东,曾勇,莫美兰,等.大Y染色体对体外受精与胚胎移植的影响[J].中华男科学杂志,2006,12(12):1088-1090.
[15]戴小凡,赵菁,刘凌云,等.染色体多态性对男性精液质量的影响[J].中国妇幼保健,2014,29:567-568.
[16] Bhasin MK.Human population cytogenetics:a review[J].Int J Hum Genet,2005,5(2):83-152.
[17]高辉.染色体多态性对IVF/ICSI-ET妊娠结局的影响[J].中国优生与遗传杂志,2016,24(2):104-105.
[18]郭威,郭艺红,苏迎春,等.男性染色体多态对IVF/ICSI-ET结局的影响[J].中国计划生育学杂志,2015,23(10):670-673.
[2] Venkatesh S,Thilagavathi J,Kumar K.Cytogenetic,Y chromosome microdeletion,sperm chromatin and oxidative stress analysis in male partners of couples experiencing recurrent spontaneous abortions[J].Arch Gynecol Obstet,2011,284(6):1577-1584.
[3] Caglayan A,Ozyazgan I,Demiryilmaz F,et al.Are heterochromatin polymorphisms associated with recurrent miscarriage?[J].Obs Gyn Res,2010,36(4):774-776.
[4]世界卫生组织.世界卫生组织人类精液检查与处理实验室手册[M].第5版.北京:人民卫生出版社,2011.
[5]余红,赵矫,赵小平,等.染色体多态性的生育异常临床效应分析[J].现代预防医学,2010,37(10):1998-1999.
[6]张思仲.染色体诊断与遗传咨询中的多态性问题和困惑[J].中华医学遗传学杂志,2010,27(6):707-709.
[7] Zhimulev IF,Beliaeva ES.Heterochromatin,gene position effect and gene silencing[J].Genetica,2003,39(2):187-201.
[8] Conly,Rieder E.The verteb rate cellk inetochore and roles during itosis[J].Trends Cell Biology,1998,8(4):310.
[9]杨丽霞,张琪瑶,孙伟.31例染色体多态性与生殖异常的关系[J].中国优生与遗传杂志,2008,16(4):38-39.
[10]刘波,李山,秦雪,等.异常孕产史夫妇染色体的分析[J].中国优生与遗传杂志,2005,13(4):45-46.
[11]程涌江,叶竞妍.Y染色体长度变异与疾病的关系[J].中国优生与遗传杂志,2005,13(6):34-35.
[12]韩芳,刘娟,翁宁,等.染色体异常对体外胚胎发育的影响[J].中华医学遗传学杂志,2007,24(1):112.
[13]周焕庚,夏家辉,张思仲.人类染色体[M].北京:科学出版社,1987,61-63.
[14]胡晓东,曾勇,莫美兰,等.大Y染色体对体外受精与胚胎移植的影响[J].中华男科学杂志,2006,12(12):1088-1090.
[15]戴小凡,赵菁,刘凌云,等.染色体多态性对男性精液质量的影响[J].中国妇幼保健,2014,29:567-568.
[16] Bhasin MK.Human population cytogenetics:a review[J].Int J Hum Genet,2005,5(2):83-152.
[17]高辉.染色体多态性对IVF/ICSI-ET妊娠结局的影响[J].中国优生与遗传杂志,2016,24(2):104-105.
[18]郭威,郭艺红,苏迎春,等.男性染色体多态对IVF/ICSI-ET结局的影响[J].中国计划生育学杂志,2015,23(10):670-673.