载甲钴胺香蕉纤维素微晶/PLGA介孔材料的制备及性能
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摘要
从香蕉树皮中提取香蕉纤维素微晶,将甲钴胺、香蕉纤维素微晶和乳酸-羟基乙酸共聚物(PLGA)制备成载甲钴胺香蕉纤维素微晶/PLGA介孔材料。运用SEM、BET、TG-MS和FTIR对产物进行了表征;采用紫外分光光度计对载甲钴胺介孔材料进行了载药率、包封率、体外释放度测定。红外谱图分析表明,所制备的香蕉纤维素微晶的主要成分为纤维素;BET法测定结果表明,载甲钴胺香蕉纤维素微晶/PLGA介孔材料的平均孔径(4V/A)(V为孔体积,A为表面积)为17.89 nm;TG-MS结果表明,载甲钴胺香蕉纤维素微晶/PLGA介孔材料分解温度约为300℃;体外释放实验表明,该材料对甲钴胺有较好的控释作用,药物释放遵循零级释放动力学方程。
A mesoporous material loading mecobalamin was prepared using mecobalamin, banana microcrystalline cellulose extracted from banana fiber and poly(lactic-co-glycolic acid) PLGA. The products were characterized by SEM, specific surface area and pore size analysis(BET), thermogravimetric analysis-mass spectrometry(TG-MS) and FTIR. The drug loading content,encapsulation efficiency, and in vitro release rate of mesoporous material loading mecobalamin were studied by UV spectrophotometry.FTIR analysis showed that the main component of the prepared banana microcrystalline cellulose was cellulose. The BET results showed that the average pore diameter〔4 V(pore volume)/A(surface area)〕of banana microcrystalline cellulose/PLGA mesoporous material loading mecobalamin was 17.89 nm. TG-MS results demonstrated that the decomposition temperature of the mesoporous material loading mecobalamin was about 300 ℃. In addition, the material had a great controlled release for mecobalamin and the drug release followed the zero-order release kinetics.
A mesoporous material loading mecobalamin was prepared using mecobalamin, banana microcrystalline cellulose extracted from banana fiber and poly(lactic-co-glycolic acid) PLGA. The products were characterized by SEM, specific surface area and pore size analysis(BET), thermogravimetric analysis-mass spectrometry(TG-MS) and FTIR. The drug loading content,encapsulation efficiency, and in vitro release rate of mesoporous material loading mecobalamin were studied by UV spectrophotometry.FTIR analysis showed that the main component of the prepared banana microcrystalline cellulose was cellulose. The BET results showed that the average pore diameter〔4 V(pore volume)/A(surface area)〕of banana microcrystalline cellulose/PLGA mesoporous material loading mecobalamin was 17.89 nm. TG-MS results demonstrated that the decomposition temperature of the mesoporous material loading mecobalamin was about 300 ℃. In addition, the material had a great controlled release for mecobalamin and the drug release followed the zero-order release kinetics.
引文
[1]Gan L,Qian M Q,Shi K Q,et al.Restorative effect and mechanism of mecobalamin on sciatic nerve crush injury in mice[J].Neural Regeneration Research,2014,9(22):1979-1984.
[2]Pang Wuyan(庞妩燕),Yan Fenglan(闫凤岚),Li Li(李理).Clinical observation on treatment of 62 cases ofdiabetic neuropathy with micopol[J].Modern Journal of Integrated Chinese and Western Medicine(现代中西医结合杂志),2000,23(12):2353-2354.
[3]Jia Lianshun(贾连顺),Li Jiashun(李家顺).Spinal trauma surgery[M].Shanghai:Shanghai far East Publishers(上海远东出版社),2000:194-196.
[4]Lv Hong(吕宏),Li Jiashun(李家顺),Ye Xiaojian(叶晓健),et al.The study of methylobalamin slowly release from DL-PLA/CHIcompound membrane as carrier in vitro[J].Journal of Spinal Surgery(脊柱外科杂志),2003,5(1):290-293.
[5]Zhang L Z,Xu L,Li G C,et al.Fabrication of high-strength mecobalamin loaded aligned silk fibroin scaffolds for guiding neuronal orienta-tion[J].Colloids and Surfaces B:Biointerfaces,2019,173:689-697.
[6]Huang Yueshan(黄岳山),Zang Songtao(臧松涛),Wu Xiaoming(吴效明).Preparation and release of polylactic-co-L-alanine[J].Chinese Journal of Pharmaceuticals(中国医药工业杂志),2005,36(3):158-160.
[7]Siqueira G,Bras J,Dufresne A.Cellulose whiskers versus microfibrils:influence of the nature of the nanoparticle and its surface functionalization on the thermal and mechanical properties of nanocomposites[J].Substance,1976,5(15):111-116.
[8]Li Xiaojian(李肖建),Liu Hongxia(刘红霞),Wei Chun(韦春),et al.Preparation and characterization of sisal cellulose microcrystal[J].Polymer Materials Science and Engineering(高分子材料科学与工程),2012,8(28):161-166.
[9]Pang Jinying(庞锦英),Mo Xianzhong(莫羡忠),Liu Yuxin(刘钰馨).Preparation and characterization of flame retardant banana fiber reinforced PLA composites[J].Chemical Industry and Engineering Progress(化工进展),2015,34(4):1050-1054.
[10]Pang Jinying(庞锦英),Mo Xianzhong(莫羡忠),Tan Dengfeng(谭登峰),et al.preparation and application ofbanana cellulose microcrystalline/polylactic acid aerogels:ZL201510399833.4[P].2015-07-09.
[11]Sun Xiaoqiang(孙小强),Liu Yang(刘扬),Chen Liang(陈亮),et al.Release of cross-linked hyaluronic acid bearing doxorubicin hydrochloride[J].Chemical Industry and Engineering Progress(化工进展),2015,34(11):4054-4058.
[12]Wei Liang(韦良).Studies on cobalt catalysts supported on mesostructured cellular silica foams in fischer-tropsch synthesis[D].Suzhou:Soochow University(苏州大学),2016.
[13]Jiang Tingda(蒋挺大).Lignin[M].Beijing:Chemical Industry Press(化学工业出版社),1991:42-46.
[14]Calvo L F,Sanchez M E,Moran A,et al.TG-MS as a technique for a better monitoring of the of the pyrolysis,gasification and combustion of two kinds of sewage sewage sludge[J].Journal of Thermal Analysis and Calorimetry,2004,78(2):587-598.
[15]Yang Feng(杨凤),Zhao Wei(赵伟),Zhang Qian(张欠).Preparation and in vitro releasing property of aspirin-montmorillonite-chitosan sustained-release microsphere[J].Journal of Functional Polymers(功能高分子学报),2010,23(1):29-34.
[16]Huang Yan(黄彦).Molecular weight determination,thermal stability kinetics ofβ-CD ploymer and the influence on water-soluble drug release[D].Guangzhou:Guangdong Pharmaceutical University(广东药学院),2007.
[17]Ye Yujie(叶玉杰),Li Fang(李芳),Ren Dequan(任德全),et al.Origin software was used to fit release rule in vitro of preparations[J].Journal of Mathematical Medicine(数理医药学杂志),2014,27(1):93-94.
[18]Ye Yujie(叶玉杰),Ren Dequan(任德全),Liu Song(刘嵩),et al.The study of origin software was used to fit the Ritger-peppas equation of the erosion type carrier drug delivery system[J].Asiapacific Traditinal Medicine(亚太传统医药),2014,10(4):62-63.
[19]Cheng Kun(程坤).Study a new kind of drug delivery system follow the zero order release-round matrix tablet[D].Nanjing:China Pharmaceutical University(中国药科大学),1999.
[2]Pang Wuyan(庞妩燕),Yan Fenglan(闫凤岚),Li Li(李理).Clinical observation on treatment of 62 cases ofdiabetic neuropathy with micopol[J].Modern Journal of Integrated Chinese and Western Medicine(现代中西医结合杂志),2000,23(12):2353-2354.
[3]Jia Lianshun(贾连顺),Li Jiashun(李家顺).Spinal trauma surgery[M].Shanghai:Shanghai far East Publishers(上海远东出版社),2000:194-196.
[4]Lv Hong(吕宏),Li Jiashun(李家顺),Ye Xiaojian(叶晓健),et al.The study of methylobalamin slowly release from DL-PLA/CHIcompound membrane as carrier in vitro[J].Journal of Spinal Surgery(脊柱外科杂志),2003,5(1):290-293.
[5]Zhang L Z,Xu L,Li G C,et al.Fabrication of high-strength mecobalamin loaded aligned silk fibroin scaffolds for guiding neuronal orienta-tion[J].Colloids and Surfaces B:Biointerfaces,2019,173:689-697.
[6]Huang Yueshan(黄岳山),Zang Songtao(臧松涛),Wu Xiaoming(吴效明).Preparation and release of polylactic-co-L-alanine[J].Chinese Journal of Pharmaceuticals(中国医药工业杂志),2005,36(3):158-160.
[7]Siqueira G,Bras J,Dufresne A.Cellulose whiskers versus microfibrils:influence of the nature of the nanoparticle and its surface functionalization on the thermal and mechanical properties of nanocomposites[J].Substance,1976,5(15):111-116.
[8]Li Xiaojian(李肖建),Liu Hongxia(刘红霞),Wei Chun(韦春),et al.Preparation and characterization of sisal cellulose microcrystal[J].Polymer Materials Science and Engineering(高分子材料科学与工程),2012,8(28):161-166.
[9]Pang Jinying(庞锦英),Mo Xianzhong(莫羡忠),Liu Yuxin(刘钰馨).Preparation and characterization of flame retardant banana fiber reinforced PLA composites[J].Chemical Industry and Engineering Progress(化工进展),2015,34(4):1050-1054.
[10]Pang Jinying(庞锦英),Mo Xianzhong(莫羡忠),Tan Dengfeng(谭登峰),et al.preparation and application ofbanana cellulose microcrystalline/polylactic acid aerogels:ZL201510399833.4[P].2015-07-09.
[11]Sun Xiaoqiang(孙小强),Liu Yang(刘扬),Chen Liang(陈亮),et al.Release of cross-linked hyaluronic acid bearing doxorubicin hydrochloride[J].Chemical Industry and Engineering Progress(化工进展),2015,34(11):4054-4058.
[12]Wei Liang(韦良).Studies on cobalt catalysts supported on mesostructured cellular silica foams in fischer-tropsch synthesis[D].Suzhou:Soochow University(苏州大学),2016.
[13]Jiang Tingda(蒋挺大).Lignin[M].Beijing:Chemical Industry Press(化学工业出版社),1991:42-46.
[14]Calvo L F,Sanchez M E,Moran A,et al.TG-MS as a technique for a better monitoring of the of the pyrolysis,gasification and combustion of two kinds of sewage sewage sludge[J].Journal of Thermal Analysis and Calorimetry,2004,78(2):587-598.
[15]Yang Feng(杨凤),Zhao Wei(赵伟),Zhang Qian(张欠).Preparation and in vitro releasing property of aspirin-montmorillonite-chitosan sustained-release microsphere[J].Journal of Functional Polymers(功能高分子学报),2010,23(1):29-34.
[16]Huang Yan(黄彦).Molecular weight determination,thermal stability kinetics ofβ-CD ploymer and the influence on water-soluble drug release[D].Guangzhou:Guangdong Pharmaceutical University(广东药学院),2007.
[17]Ye Yujie(叶玉杰),Li Fang(李芳),Ren Dequan(任德全),et al.Origin software was used to fit release rule in vitro of preparations[J].Journal of Mathematical Medicine(数理医药学杂志),2014,27(1):93-94.
[18]Ye Yujie(叶玉杰),Ren Dequan(任德全),Liu Song(刘嵩),et al.The study of origin software was used to fit the Ritger-peppas equation of the erosion type carrier drug delivery system[J].Asiapacific Traditinal Medicine(亚太传统医药),2014,10(4):62-63.
[19]Cheng Kun(程坤).Study a new kind of drug delivery system follow the zero order release-round matrix tablet[D].Nanjing:China Pharmaceutical University(中国药科大学),1999.