摘要
以‘金都一号’火龙果新萌芽的茎段为外植体,进行组培技术研究,以建立‘金都一号’火龙果组培快繁体系。结果表明:外植体最佳消毒组合为75%酒精20s+0.1%升汞10~15 min,污染率55.70%;初代培养基为MS+6-BA 8.0 mg/L+NAA 0.1 mg/L,诱导率为85.47%;增殖培养基为MS+6-BA 2.0 mg/L,增殖系数为7.13;生根培养基为MS+ABT60.5 mg/L+IBA 0.5 mg/L+NAA 0.1 mg/L,生根率达到98.56%,苗壮根粗,分枝少。
Segments of newly sprouted stem of pitaya 'Jindu No. 1'(Hylocereus undatus) were used as explants for tissue culture, based on which a rapid propagation system was established. The result showed that the best disinfection combination for the explants was 75% alcohol 20 s + 0.1% mercury chloride 10-15 min and had a contamination rate of 55.70 %. The primary culture medium was MS +6-BA 8.0 mg/L +NAA 0.1 mg/L, and the induction rate was 85.47 %. The proliferating medium was MS +6-BA 2.0 mg/L with a proliferation coefficient of 7.13. The rooting medium was MS + ABT60.5 mg/L + IBA 0.5 mg/L+NAA 0.1 mg/L, the rooting rate was 98.56 %, and the plantlets had thick roots and few branches.
引文
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