葡萄糖、乳酸钠对丙酸杆菌生长的影响
|
摘要
对费氏丙酸杆菌(Propionibacterium freudenreichii CS1420)发酵培养基的碳源进行优化,旨在提高菌体密度。结果表明,以乳酸钠作为单一碳源时,当乳酸钠添加量为32 g/L时,菌体生长量最大,OD600 nm为1.95;以葡萄糖作为单一碳源时,当葡萄糖添加量为8 g/L时,菌体生长量最大,OD600 nm为1.242;以乳酸钠和葡萄糖作为复合碳源时,当碳源添加量为18.8g/L乳酸钠+1.2g/L葡萄糖时,菌体生长量最大,OD600nm为2.06,这说明,复合碳源可以有效的提高菌体密度。
The effects of the carbon sources of the medium on the growth of Propionibacterium freudenreichii CS1420 were studied in order to increase the cell density. The results showed:the growth rate of the cell was the largest when sodium lactate was used as a single carbon source and the amount of sodium lactate was 32g/L,OD600 nmwas 1.95. The growth rate of the cell was the largest when glucose was used as a single carbon source and the amount of sodium lactate was 8 g/L,OD600 nmwas 1.242. The growth rate of the cell was the largest when glucose and sodium lactate were used as compounded carbons and the amount of sodium lactate was 18.8 g/L sodi-um lactate,1.2 g/L glucose,OD600 nmwas 2.06. This showed that the composite carbon source can effectively improve the cell density.
The effects of the carbon sources of the medium on the growth of Propionibacterium freudenreichii CS1420 were studied in order to increase the cell density. The results showed:the growth rate of the cell was the largest when sodium lactate was used as a single carbon source and the amount of sodium lactate was 32g/L,OD600 nmwas 1.95. The growth rate of the cell was the largest when glucose was used as a single carbon source and the amount of sodium lactate was 8 g/L,OD600 nmwas 1.242. The growth rate of the cell was the largest when glucose and sodium lactate were used as compounded carbons and the amount of sodium lactate was 18.8 g/L sodi-um lactate,1.2 g/L glucose,OD600 nmwas 2.06. This showed that the composite carbon source can effectively improve the cell density.
引文
[1]Muto M.Chemical technology for preservation of food products:food preservative,ε-poly-L-lysine[J].Boukin Boubai,2009,37:765-772
[2]邢海丽,辛嘉英,王艳,等.微生物源天然食品防腐剂的研究进展[J].食品安全质量检测学报,2015(10):3889-3894
[3]岳昊博,岳喜庆,李靖,等.纳他霉素(Natamycin)的特性、应用及生产和研究状况[J].食品科技,2007,32(3):162-166
[4]Talarico T L,Casas I A,Chung T C,et al.Production and isolation of reuterin,a growth inhibitor produced by Lactobacillus reuteri[J].Antimicrobial Agents&Chemotherapy,1988,32(12):1854-1858
[5]Leversee J A,Glatz B A.Detection of the bacteriocin propionicin PLG-1 with polyvalent anti-PLG-1 antiserum[J].Applied&Environmental Microbiology,2001,67(5):2235-2239
[6]冯晗,荣邵丰,贾彩凤,等.丙酸杆菌代谢物对恶臭假单胞菌抑制活性[J].兰州大学学报(自然科学版),2008,44(2):58-62
[7]荣绍丰,常忠义,高红亮,等.不同丙酸杆菌代谢物对酵母菌抑菌作用的研究[J].食品工业,2009(1):1-4
[8]Danilova I V,Bykovchenko T V,Ryzhkova E P.A positive effect of Propionibacterium freudenreichii,on the growth of Saccharomyces cerevisiae,during their cocultivation[J].Applied Biochemistry&Microbiology,2006,42(4):378-383
[9]Hajfarajollah H,Mokhtarani B,Noghabi K A.Newly Antibacterial and Antiadhesive Lipopeptide Biosurfactant Secreted by a Probiotic Strain,Propionibacterium Freudenreichii[J].Applied Biochemistry&Biotechnology,2014,174(8):2725-2740
[10]梁新乐,刘爱琴,钟立人.丙酸杆菌的研究进展及其在食品发酵工业中的应用[J].食品研究与开发,2006,27(6):170-172
[11]诸晓强,陈乐乐.乳品丙酸杆菌素作为生物防腐剂的研究进展[J].中国乳品工业,2006,34(9):40-43
[12]贾彩凤.丙酸杆菌代谢物做为食品防腐剂的研究[D].上海:华东师范大学,2005
[13]郑丽雪,王荆楚,时慧佳,等.一株费氏丙酸杆菌生长特性及其代谢物抑菌作用分析[J].食品科学,2015,36(15):163-166
[14]Martínez-Campos R,Torre M D L.Production of propionate by fedbatch fermentation of Propionibacterium acidipropionici using mixed feed of lactate and glucose[J].Biotechnology Letters,2002,24(6):427-431
[15]陈玉梅,常忠义,王疆元,等.乳酸钠和葡萄糖对薛氏丙酸杆菌生长及代谢物抑菌活性的影响[J].西北农林科技大学学报(自然科学版),2007,35(2):178-182
[2]邢海丽,辛嘉英,王艳,等.微生物源天然食品防腐剂的研究进展[J].食品安全质量检测学报,2015(10):3889-3894
[3]岳昊博,岳喜庆,李靖,等.纳他霉素(Natamycin)的特性、应用及生产和研究状况[J].食品科技,2007,32(3):162-166
[4]Talarico T L,Casas I A,Chung T C,et al.Production and isolation of reuterin,a growth inhibitor produced by Lactobacillus reuteri[J].Antimicrobial Agents&Chemotherapy,1988,32(12):1854-1858
[5]Leversee J A,Glatz B A.Detection of the bacteriocin propionicin PLG-1 with polyvalent anti-PLG-1 antiserum[J].Applied&Environmental Microbiology,2001,67(5):2235-2239
[6]冯晗,荣邵丰,贾彩凤,等.丙酸杆菌代谢物对恶臭假单胞菌抑制活性[J].兰州大学学报(自然科学版),2008,44(2):58-62
[7]荣绍丰,常忠义,高红亮,等.不同丙酸杆菌代谢物对酵母菌抑菌作用的研究[J].食品工业,2009(1):1-4
[8]Danilova I V,Bykovchenko T V,Ryzhkova E P.A positive effect of Propionibacterium freudenreichii,on the growth of Saccharomyces cerevisiae,during their cocultivation[J].Applied Biochemistry&Microbiology,2006,42(4):378-383
[9]Hajfarajollah H,Mokhtarani B,Noghabi K A.Newly Antibacterial and Antiadhesive Lipopeptide Biosurfactant Secreted by a Probiotic Strain,Propionibacterium Freudenreichii[J].Applied Biochemistry&Biotechnology,2014,174(8):2725-2740
[10]梁新乐,刘爱琴,钟立人.丙酸杆菌的研究进展及其在食品发酵工业中的应用[J].食品研究与开发,2006,27(6):170-172
[11]诸晓强,陈乐乐.乳品丙酸杆菌素作为生物防腐剂的研究进展[J].中国乳品工业,2006,34(9):40-43
[12]贾彩凤.丙酸杆菌代谢物做为食品防腐剂的研究[D].上海:华东师范大学,2005
[13]郑丽雪,王荆楚,时慧佳,等.一株费氏丙酸杆菌生长特性及其代谢物抑菌作用分析[J].食品科学,2015,36(15):163-166
[14]Martínez-Campos R,Torre M D L.Production of propionate by fedbatch fermentation of Propionibacterium acidipropionici using mixed feed of lactate and glucose[J].Biotechnology Letters,2002,24(6):427-431
[15]陈玉梅,常忠义,王疆元,等.乳酸钠和葡萄糖对薛氏丙酸杆菌生长及代谢物抑菌活性的影响[J].西北农林科技大学学报(自然科学版),2007,35(2):178-182