东方伊萨酵母FJ-J-3发酵特性的研究
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摘要
该实验研究了东方伊萨酵母(Issatchenkia orientalis)FJ-J-3菌株在不同初始葡萄糖含量、培养温度条件下细胞生长、葡萄糖利用及产乙醇情况。结果表明,在培养基中含有50 g/L葡萄糖时菌体生长量较高,生长速率最快;且乙醇产量较高,产率最高。在培养温度为28℃时菌体生长量最高,且乙醇产量最高。菌株FJ-J-3能够耐受16%vol的乙醇,且在72 h时细胞仍能正常存活。
The cell growth,glucose utilization and ethanol production of Issatchenkia orientalis FJ-J-3 were studied at the conditions of different initial glucose contents and culture temperatures.Results showed that I.orientalis grew well and the growth rate was the fastest in the medium containing glucose 50 g/L,and the ethanol production and productive rate were the highest.At the condition of culture temperature 28 ℃,the cell growth and the ethanol production were the highest.The ethanol tolerance of strain FJ-J-3 was good; it could tolerate 16%vol ethanol and grew well for 72 h.
The cell growth,glucose utilization and ethanol production of Issatchenkia orientalis FJ-J-3 were studied at the conditions of different initial glucose contents and culture temperatures.Results showed that I.orientalis grew well and the growth rate was the fastest in the medium containing glucose 50 g/L,and the ethanol production and productive rate were the highest.At the condition of culture temperature 28 ℃,the cell growth and the ethanol production were the highest.The ethanol tolerance of strain FJ-J-3 was good; it could tolerate 16%vol ethanol and grew well for 72 h.
引文
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[3]TEIXEIRA M C,RAPOSO L R,MIRA N P,et al Genome-wide identification of Saccharomyces cerevisiae genes required for maximal tolerance to ethanol[J].Appl Environ Microbiol,2009,75(18):5761-5772.
[4]于洋,粟春燕,陈晶瑜.3株葡萄酒非酿酒酵母的生长与发酵特性[J].食品科学,2015,36(19):107-109.
[5]张敏,毛健,黄桂东.清酒酿酒酵母酒精耐受机理的研究进展[J].食品工业科技,2012,33(20):342-346.
[6]张强,郭元,韩德明.酿酒酵母乙醇耐受性的研究进展[J].化工进展,2014,33(1):187-189.
[7]王灏,王航,孟春,等.基因组改组技术选育耐高温、耐高乙醇酿酒酵母菌株的研究[J].微生物学通报,2007,34(4):705-708.
[8]娜日苏,苏亚拉图,高凤芹.耐高温耐酸耐酒精酵母的筛选与鉴定[J].草业科学,2013,30(10):1625-1632.
[9]姚淑敏,闫华文,陈璐.不同甜酒曲中可培养真菌的多样性分析[J].中国酿造,2015,34(12):48-54.
[10]闫华文.甜酒曲中真菌多样性的研究及甜酒酿营养成分分析[D].曲阜:曲阜师范大学,2015.
[11]覃香香,张厚瑞,蔡爱华,等.东方伊萨酵母降解木糖醇发酵抑制物研究[J].广西科学,2010,17(4):358-362.
[12]张慕明,余志晟,张洪勋,等.东方伊萨酵母YP-1对染料活性艳红K-2BP的脱色[J].中国环境科学,2009,29(12):1266-1271.
[13]TONDEE T,SIRIANUNTAPIBOON S,OHMOMO S.Decolorization of molasses wastewater by yeast strain,Issatchenkia orientalis No.SF9-246[J].Bioresource Technol,2008,99:5511-5519.
[14]王风芹,汪媛媛,陶西,等.耐高温东方伊萨酵母乙醇发酵特性[J].农业工程学报,2014,30(3):180-187.
[15]KWON Y J,MA AZ,LI Q,et al.Effect of lignocellulosic inhibitory compounds on growth and ethanol fermentation of newly-isolated thermotolerant Issatchenkia orientalis[J].Bioresource Technol,2011(102):8099-8104.
[16]KWON Y J,WANG F,LIU C Z.Deep-bed solid state fermentation of sweet sorghum stalk to ethanol by thermotolerant Issatchenkia orientalis IPE 100[J].Bioresource Technol,2011(102):11262-11265.
[17]杨革.微生物学实验教程[M].北京:科学出版社,2009:21-26.
[18]王继花.盐胁迫下酿酒酵母生理生化特性的研究[D].大连:大连工业大学,2008.
[19]牟建楼,王颉,陈志周,等.木糖发酵液中乙醇的气相色谱法测定[J].酿酒,2005,32(1):77-78.