荧光假单胞菌与红绒盖牛肝菌共接种对杨树氮代谢和矿质元素含量的影响
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摘要
【目的】探究荧光假单胞菌与红绒盖牛肝菌共接种条件下NL-895杨植株氮代谢及矿质元素含量的响应,在养分代谢水平上揭示溶磷细菌与外生菌根菌互作对杨树生长的影响,为杨树复合菌剂的开发与应用提供理论依据。【方法】对2年生NL-895盆栽菌共接种荧光假单胞菌JW-JS1和红绒盖牛肝菌(Xc),测定氮代谢指标可溶性蛋白质含量、硝态氮含量和硝酸还原酶活性以及植株矿质元素含量。【结果】无论JW-JS1或Xc单接种,还是JW-JS1与Xc共接种均能显著提高NL-895杨树叶片可溶性蛋白、硝态氮含量和硝酸还原酶活性以及植株的矿质元素含量,且共接种处理优于各单接种处理。共接种30天后NL-895杨的可溶性蛋白质含量、硝态氮含量和硝酸还原酶活性分别比CK增长20.19%、79.99%和33.08%,共接种90天后增长76.76%、106.62%和34.86%,共接种150天后增长137.99%、50.70%和59.89%,植株内P、Mg、K、Ca、Mn和Zn含量分别比CK增长71.66%、23.67%、49.71%、22.90%、84.57%和151.56%。相关性分析发现,共接种30天后NL-895杨苗高和茎粗与硝态氮含量显著正相关(P<0.05);共接种90天后NL-895杨苗茎粗与可溶性蛋白含量显著正相关(P<0.05);共接种150天后NL-895杨苗高与硝酸还原酶活性、Mg含量、K含量显著正相关(P<0.05),NL-895杨茎粗与P含量显著正相关(P<0.05)、K含量极显著正相关(P<0.01)。【结论】荧光假单胞菌JW-JS1与红绒盖牛肝菌共接种显著改善NL-895杨的氮代谢功能和矿质元素的吸收从而促进NL-895杨的生长。
【Objective】This paper aimed to explore the response of Populus×euramericana cv.‘Nanlin-895'(NL-895) to nitrogen metabolism and mineral elements under co-inoculation with P. fluorescent and X. chrysenteron, to reveal the effects of interaction between phosphate-solubilizing bacteria and ectomycorrhizal fungi on poplar growth at the level of nutrient metabolism, so as to provide a theoretical basis for developing and exploiting the compound microbial fertilizer for poplar. 【Method】 A pot experiment was conducted to investigate the effects of co-inoculation with Pseudomonas fluorescent JW-JS1 and Xerocomus chrysenteron on the nitrogen metabolism(soluble protein contents, nitrate nitrogen contents and nitrate reductase activity) and mineral element contents of NL-895 poplar in the greenhouse. 【Result】 Both JW-JS1 or Xc single inoculation and JW-JS1 and Xc co-inoculation significantly increased the soluble protein contents, nitrate nitrogen contents and nitrate reductase activity of NL-895 Poplar, and improved its mineral element content, and then the treatment effect of co-inoculation was higher than single inoculations and the control. The soluble protein content, the nitrate nitrogen content and the nitrate reductase activity of NL-895 poplar increased 20.19%, 79.99%, and 33.08%, respectively after 30 days of co-inoculation, 76.76%, 106.62%, and 34.86% after 90 days of co-inoculation and 137.99%, 50.70%, and 59.89% after 150 days of co-inoculation, compared with the control. The contents of P, Mg, K, Ca, Mn and Zn of NL-895 Poplar increased 71.66%, 23.67%, 49.71%, 22.90%, 84.57% and 151.56%, respectively compared with the control after 150 days of co-inoculation. Correlation analysis showed that the height and stem diameter of NL-895 seedlings were positively correlated with nitrate nitrogen content after 30 days of co-inoculation(P<0.05), and the height of NL-895 seedlings was positively correlated with soluble protein content after 90 days of co-inoculation(P<0.05). The height of NL-895 seedlings was positively correlated with nitrate reductase activity and K contents(P<0.05) after 150 days of co-inoculation, and the stem diameter of NL-895 seedlings was positively correlated with P contents(P<0.05) and K contents(P<0.01) after 150 days of co-inoculation. 【Conclusion】 The co-inoculation of Pseudomonas fluorescent JW-JS1 and Xerocomus chrysenteron significantly improved the nitrogen metabolism and mineral element absorption of NL-895 poplar, thus promoting the growth of NL-895 poplar.
【Objective】This paper aimed to explore the response of Populus×euramericana cv.‘Nanlin-895'(NL-895) to nitrogen metabolism and mineral elements under co-inoculation with P. fluorescent and X. chrysenteron, to reveal the effects of interaction between phosphate-solubilizing bacteria and ectomycorrhizal fungi on poplar growth at the level of nutrient metabolism, so as to provide a theoretical basis for developing and exploiting the compound microbial fertilizer for poplar. 【Method】 A pot experiment was conducted to investigate the effects of co-inoculation with Pseudomonas fluorescent JW-JS1 and Xerocomus chrysenteron on the nitrogen metabolism(soluble protein contents, nitrate nitrogen contents and nitrate reductase activity) and mineral element contents of NL-895 poplar in the greenhouse. 【Result】 Both JW-JS1 or Xc single inoculation and JW-JS1 and Xc co-inoculation significantly increased the soluble protein contents, nitrate nitrogen contents and nitrate reductase activity of NL-895 Poplar, and improved its mineral element content, and then the treatment effect of co-inoculation was higher than single inoculations and the control. The soluble protein content, the nitrate nitrogen content and the nitrate reductase activity of NL-895 poplar increased 20.19%, 79.99%, and 33.08%, respectively after 30 days of co-inoculation, 76.76%, 106.62%, and 34.86% after 90 days of co-inoculation and 137.99%, 50.70%, and 59.89% after 150 days of co-inoculation, compared with the control. The contents of P, Mg, K, Ca, Mn and Zn of NL-895 Poplar increased 71.66%, 23.67%, 49.71%, 22.90%, 84.57% and 151.56%, respectively compared with the control after 150 days of co-inoculation. Correlation analysis showed that the height and stem diameter of NL-895 seedlings were positively correlated with nitrate nitrogen content after 30 days of co-inoculation(P<0.05), and the height of NL-895 seedlings was positively correlated with soluble protein content after 90 days of co-inoculation(P<0.05). The height of NL-895 seedlings was positively correlated with nitrate reductase activity and K contents(P<0.05) after 150 days of co-inoculation, and the stem diameter of NL-895 seedlings was positively correlated with P contents(P<0.05) and K contents(P<0.01) after 150 days of co-inoculation. 【Conclusion】 The co-inoculation of Pseudomonas fluorescent JW-JS1 and Xerocomus chrysenteron significantly improved the nitrogen metabolism and mineral element absorption of NL-895 poplar, thus promoting the growth of NL-895 poplar.
引文
蔡红光, 米国华, 陈范骏, 等.2010. 玉米叶片SPAD值、全氮及硝态氮含量的品种间变异. 植物营养与肥料学报, 16(4): 866-873.(Cai H G, Mi G H, Chen F J, et al. 2010. Genotypic variation of leaf SPAD value, nitrogen and nitrate content in maize. Plant Nutrition and Fertilizer Science, 16(4): 866-873. [in Chinese])
陈新平, 邹春琴, 刘亚萍, 等. 2000. 菠菜不同品种累积硝酸盐能力的差异及其原因. 植物营养与肥料学报, 6(1): 30-34.(Chen X P, Zou C Q, Liu Y P, et al. 2000. The nitrate content difference and the reason among four spinach varieties. Plant nutrition and fertilizer Science, 6(1): 30-34. [in Chinese])
段巍巍, 赵红梅, 郭程瑾, 等. 2007. 夏玉米光合特性对氮素用量的反应. 作物学报, 33(6): 949-954.(Duan W W, Zhao H M, Guo C J, et al. 2007. Responses of photosynthesis characteristics to nitrogen application rates in summer maize(Zea mays L.). Acta Agronomica Sinica, 33(6): 949-954. [in Chinese])
孔令刚, 刘福德, 王华田, 等. 2006. 施肥对I-107杨树人工林土壤根际效应的影响. 中国水土保持科学,4(5): 60-65.(Kong L G, Liu F D, Wang H T, et al. 2006. Effect of fertilization on soil microflora and enzyme rhizosphere effect in poplar forestland. Science of Soil and Water Conservation, 4(5): 60-65.[in Chinese])
李 倩, 吴小芹, 叶建仁. 2015. 一种马尾松菌根辅助细菌—短芽孢杆菌的筛选及鉴定. 林业科学,51(5): 159-164.(Li Q, Wu X Q, Ye J R. 2015. Isolation and identification of a mycorrhiza helper bacteria (MHB) — Brevibacillus reuszeri on Pinus massoniana roots. Scientia Silvae Sinicae, 51(5): 159-164. [in Chinese])
李 莎, 唐 明, 黄玲玲. 2011. 接种乳黄粘盖牛肝菌和荧光假单胞菌对油松苗生长及猝倒病的影响. 西北植物学报, 31(7): 1384-1389.(Li S, Tang M, Huang L L. 2011. Effect of inoculating Suillus lactifluus and Pseudomonas fluorescens to Pinus tabulaeformis growth and resistant of damping-off. Acta Botanica Boreali-Occidentalia Sinica, 31(7): 1384-1389. [in Chinese])
李熙英, 刘继生, 黄世臣. 2010. 不同施肥条件下丛枝菌根对1年生杨树扦插苗生长的影响. 林业科技, 35(1): 22-25.(Li X Y, Liu J S, Huang S Z. 2010. Effect on growth of one -year poplar seeding with VAM in different fertilization methods. Forestry Science & Technology, 35(1): 22-25.[in Chinese])
梁 军, 张 颖, 贾秀贞, 等. 2003. 外生菌根菌对杨树生长及抗逆性指标的效应. 南京林业大学学报:自然科学版, 27(4): 39-43.(Liang J, Zhang Y, Jia X Z, et al. 2003. Effects of ectomycorrhizae on growth and resistance of poplar. Journal of Nanjing Forestry University:Natural Science Edition, 27(4): 39-43. [in Chinese])
刘 辉, 吴小芹, 任嘉红,等. 2013. 一株荧光假单胞菌的溶磷特性及其对杨树的促生效果. 林业科学, 49(9): 112-118.(Liu H, Wu X Q, Ren J H, et al. 2013. Phosphate-dissolving characteristics and growth promoting effect of Pseudomonads fluorescent JW-JS1 on poplar seedlings. Scientia Silvae Sinicae, 49(9): 112-118. [in Chinese])
刘 辉,吴小芹,任嘉红,等. 2012. 荧光假单胞菌及红绒盖牛肝菌接种对 NL-895 杨生长和光合特征的影响. 西部林业科学, 41(1): 53-59.(Liu H, Wu X Q, Ren J H, et al. 2012. Effect of Inoculating Pesudomonas fluorenscens and Xerocomus chrysenteron on growth and photosynthetic characteristics of NL-895 poplar. Journal of West China Forestry Science, 41(1): 53-59. [in Chinese])
刘方春,邢尚军, 马海林, 等. 2013. 根际促生细菌(PGPR)对冬枣根际土壤微生物数量及细菌多样性影响. 林业科学, 49(8): 75-80.(Liu F C, Xing S J, Ma H L, et al. 2003. Effect of plant growth-promoting rhizobacteria (PGPR) on the microorganism population and bacterial diversity in Ziziphus jujuba rhizosphere soil. Scientia Silvae Sinicae, 49(8): 75-80. [in Chinese])
刘雅荣, 王世绩, 周国璋, 等. 1988. 杨树苗木叶硝酸还原酶活力的初步研究. 林业科学研究, 1(3): 340-344.(Liu Y R, Wang S J, Zhou G Z, et al. 1988. A preliminary study on nitrate reductase in the leaves of poplar. Forest research, 1(3): 340-344. [in Chinese])
刘祖祺, 张石城. 1994. 植物抗性生理学. 北京: 农业出版社, 371-372.(Liu Z Q, Zhang S C. 1994. The physiology of plant resistance. Beijing: Agricultural Press, 371-372. [in Chinese])
马 磊, 吴小芹. 2007. 九种外生菌根菌与杨树苗木的菌根化研究. 南京林业大学学报:自然科学版, 31(6): 29-33.(Ma L, Wu X Q. 2007. Research on mycorrhizal formation of nine ectomycorrhizal fungi with poplar seedlings. Journal of Nanjing Forestry University:Natural Science Edition, 31(6): 29-33. [in Chinese])
马光恕,刘 涛,廉 华,等.2013. 氮钾配施对麦瓶草(面条菜)品质性状及相关酶活性的影响. 中国土壤与肥料,(4): 77-82.(Ma G S, Liu T, Lian H, et al. 2013. Effects of combined application of nitrogen and potassium on quality traits and related enzymes activity of Silene conoidea L. (noodles greens). Soil and Fertilizer Sciences in China,(4): 77-82. [in Chinese])
潘超美, 郭庆荣, 邱桥姐, 等. 2000. VAM 菌根真菌对玉米生长及根际土壤微生态环境的影响. 土壤与环境, 9(4): 304-306.(Pang C M, Guo Q R, Qiu Q J, et al. 2000. Effect of VAM fungus on the growth of core and micro-ecological environment of core rhizosphere. Soil and Environmental Sciences, 9(4): 304-306. [in Chinese])
秦芳玲, 田中民. 2009. 同时接种解磷细菌与丛枝菌根真菌对低磷土壤红三叶草养分利用的影响. 西北农林科技大学学报:自然科学版, 37(6): 151-157.(Qin F L, Tian Z M. 2009. Effect of co-inoculation with arbuscular mycorrhizal fungi and four different phosphate-solubilizing bacteria on nutrients uptake of red clover in a low phosphorus soil. Journal of Northwest A&F University:Natural Science Edition, 37(6): 151-157. [in Chinese])
任嘉红, 李 浩, 刘 辉, 等. 2016. 吡咯伯克霍尔德氏菌JK-SH007对杨树根际微生物数量及功能多样性的影响. 林业科学, 52(5): 126-133.(Ren J H, Li H, Liu H, et al. 2016. Influence of Burkholderia pyrrocinia JK-SH007 on the microbial population and functional diversity of microbial communities in the rhizosphere soil of poplar. Scientia Silvae Sinicae, 52(5): 126-133. [in Chinese])
盛江梅, 吴小芹, 侯亮亮.2014.黑松—黄色须腹菌共生体根际菌根辅助细菌的筛选及鉴定. 东北林业大学学报, 42(5): 110-114.(Sheng J M, Wu X Q, Hou L L. 2014. Isolating and identifying mycorrhiza helper bacteria from the rhizosphere soil of Pinus thunbergii inoculated with Rhizipogen luteous. Journal of Northeast Forestry University, 42(5): 110-114. [in Chinese])
宋 微, 吴小芹. 2011. 外生菌根真菌对NL-895杨光合作用的影响. 西北植物学报, 31(7): 1474-1478.(Song W, Wu X Q. 2011. Effect of ectomycorrhizal fungi on photosynthesis of poplar NL-895. Acta Botanica Boreali-Occidentalia Sinica, 31(7): 1474-1478. [in Chinese])
唐 菁. 2006. 杨树施用细菌肥料的增长效应及作用机理研究. 北京:中国林业科学研究院博士论文.(Tang J. 2006. Studies on promotion of bacterial fertilizers on poplar growth and its mechanism. Beijing: PhD thesis of Chinese Academy of Forestry. [in Chinese])
姚如斌,吴小芹. 2012. 高效解磷细菌与菌根真菌菌剂交互作用对杨树的促生效应. 南京林业大学学报:自然科学版, 36(5): 170-173.(Yao R B, Wu X Q. 2012. Interaction between high effective phosphate-solubilizing bacteria and mycorrhizal fungi and its effects on poplar growth. Journal of Nanjing Forestry University:Natural Science Edition, 36(5): 170-173. [in Chinese])
曾丽琼. 2010. 几种优良外生菌根菌的应用和胶丸菌剂的制备. 南京:南京林业大学硕士论文.(Zeng L Q. 2010. Field application of ectomcorrhizal fungi and study on the process of capsules microbial agent. Nanjing: MS thesis of Nanjing Forestry University. [in Chinese])
赵秀云,韩素芬. 2001. 固氮细菌与杨树的联合固氮作用. 南京林业大学学报:自然科学版, 25(4): 17-20.(Zhao X Y, Han S F. 2001. Nitrogen fixing bacteria and poplar’s associative nitrogen fixation. Journal of Nanjing Forestry University:Natural Science Edition, 25(4): 17-20.[in Chinese])
Adesemoye A O, Torbert H A, Kloepper J W. 2008. Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Canadian Journal of Microbiology, 54: 876-886.
Aspray T J, Frey-Klett P, Jones J E. 2006. Mycorrhization helper bacteria: a case of specificity for altering ectomycorrhiza architecture but not ectomycorrhiza formation. Mycorrhiza, 16(8): 533-541.
Duponnois R, Plenchette C. 2003. A mycorrhiza helper bacterium enhances ectomycorrhizal and endomycorrhizal symbiosis of Australian Acacia species. Mycorrhiza, 13(2): 85-91.
Founoune H, Duponnois R, Meyer J M, et al. 2002. Interactions between ectomycorrhizal symbiosis and fluorescent pseudomonads on Acacia holosericea: isolation of mycorrhiza helper bacteria (MHB) from a Soudano-Sahelian soil. FEMS Microbiology Ecology, 41(1): 37-46.
Hassani F, Ardakani M, Asgharzade A, et al. 2014. Efficiency of mycorrhizal fungi and phosphate solubilizing bacteria on phosphorus uptake and chlorophyll index in potato plantlets. International Journal of Biosciences, 4(1): 244-251.
Kim K Y, Jordan D. McDonald G.A. 1998. Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity. Biology and Fertility of Soils, 26: 79-87.
Liu H, Wu X Q, Ren J H. and Ye J R. 2011. Isolation and identification of phosphobacteria in poplar rhizosphere rom different regions of China. Pedosphere, 21(1): 90-97.
Nadeem S M, Ahmad M, Zahir Z, et al. 2014. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnology Advances, 32(2): 429-448.
Zaidi A, Khan M S. 2007. Stimulatory effects of dual inoculation with phosphate-solubilizing microorganisms and arbuscular mycorrhizal fungus on chickpea. Australian Journal of Experimental Agriculture, 47(8): 1016-1022.
陈新平, 邹春琴, 刘亚萍, 等. 2000. 菠菜不同品种累积硝酸盐能力的差异及其原因. 植物营养与肥料学报, 6(1): 30-34.(Chen X P, Zou C Q, Liu Y P, et al. 2000. The nitrate content difference and the reason among four spinach varieties. Plant nutrition and fertilizer Science, 6(1): 30-34. [in Chinese])
段巍巍, 赵红梅, 郭程瑾, 等. 2007. 夏玉米光合特性对氮素用量的反应. 作物学报, 33(6): 949-954.(Duan W W, Zhao H M, Guo C J, et al. 2007. Responses of photosynthesis characteristics to nitrogen application rates in summer maize(Zea mays L.). Acta Agronomica Sinica, 33(6): 949-954. [in Chinese])
孔令刚, 刘福德, 王华田, 等. 2006. 施肥对I-107杨树人工林土壤根际效应的影响. 中国水土保持科学,4(5): 60-65.(Kong L G, Liu F D, Wang H T, et al. 2006. Effect of fertilization on soil microflora and enzyme rhizosphere effect in poplar forestland. Science of Soil and Water Conservation, 4(5): 60-65.[in Chinese])
李 倩, 吴小芹, 叶建仁. 2015. 一种马尾松菌根辅助细菌—短芽孢杆菌的筛选及鉴定. 林业科学,51(5): 159-164.(Li Q, Wu X Q, Ye J R. 2015. Isolation and identification of a mycorrhiza helper bacteria (MHB) — Brevibacillus reuszeri on Pinus massoniana roots. Scientia Silvae Sinicae, 51(5): 159-164. [in Chinese])
李 莎, 唐 明, 黄玲玲. 2011. 接种乳黄粘盖牛肝菌和荧光假单胞菌对油松苗生长及猝倒病的影响. 西北植物学报, 31(7): 1384-1389.(Li S, Tang M, Huang L L. 2011. Effect of inoculating Suillus lactifluus and Pseudomonas fluorescens to Pinus tabulaeformis growth and resistant of damping-off. Acta Botanica Boreali-Occidentalia Sinica, 31(7): 1384-1389. [in Chinese])
李熙英, 刘继生, 黄世臣. 2010. 不同施肥条件下丛枝菌根对1年生杨树扦插苗生长的影响. 林业科技, 35(1): 22-25.(Li X Y, Liu J S, Huang S Z. 2010. Effect on growth of one -year poplar seeding with VAM in different fertilization methods. Forestry Science & Technology, 35(1): 22-25.[in Chinese])
梁 军, 张 颖, 贾秀贞, 等. 2003. 外生菌根菌对杨树生长及抗逆性指标的效应. 南京林业大学学报:自然科学版, 27(4): 39-43.(Liang J, Zhang Y, Jia X Z, et al. 2003. Effects of ectomycorrhizae on growth and resistance of poplar. Journal of Nanjing Forestry University:Natural Science Edition, 27(4): 39-43. [in Chinese])
刘 辉, 吴小芹, 任嘉红,等. 2013. 一株荧光假单胞菌的溶磷特性及其对杨树的促生效果. 林业科学, 49(9): 112-118.(Liu H, Wu X Q, Ren J H, et al. 2013. Phosphate-dissolving characteristics and growth promoting effect of Pseudomonads fluorescent JW-JS1 on poplar seedlings. Scientia Silvae Sinicae, 49(9): 112-118. [in Chinese])
刘 辉,吴小芹,任嘉红,等. 2012. 荧光假单胞菌及红绒盖牛肝菌接种对 NL-895 杨生长和光合特征的影响. 西部林业科学, 41(1): 53-59.(Liu H, Wu X Q, Ren J H, et al. 2012. Effect of Inoculating Pesudomonas fluorenscens and Xerocomus chrysenteron on growth and photosynthetic characteristics of NL-895 poplar. Journal of West China Forestry Science, 41(1): 53-59. [in Chinese])
刘方春,邢尚军, 马海林, 等. 2013. 根际促生细菌(PGPR)对冬枣根际土壤微生物数量及细菌多样性影响. 林业科学, 49(8): 75-80.(Liu F C, Xing S J, Ma H L, et al. 2003. Effect of plant growth-promoting rhizobacteria (PGPR) on the microorganism population and bacterial diversity in Ziziphus jujuba rhizosphere soil. Scientia Silvae Sinicae, 49(8): 75-80. [in Chinese])
刘雅荣, 王世绩, 周国璋, 等. 1988. 杨树苗木叶硝酸还原酶活力的初步研究. 林业科学研究, 1(3): 340-344.(Liu Y R, Wang S J, Zhou G Z, et al. 1988. A preliminary study on nitrate reductase in the leaves of poplar. Forest research, 1(3): 340-344. [in Chinese])
刘祖祺, 张石城. 1994. 植物抗性生理学. 北京: 农业出版社, 371-372.(Liu Z Q, Zhang S C. 1994. The physiology of plant resistance. Beijing: Agricultural Press, 371-372. [in Chinese])
马 磊, 吴小芹. 2007. 九种外生菌根菌与杨树苗木的菌根化研究. 南京林业大学学报:自然科学版, 31(6): 29-33.(Ma L, Wu X Q. 2007. Research on mycorrhizal formation of nine ectomycorrhizal fungi with poplar seedlings. Journal of Nanjing Forestry University:Natural Science Edition, 31(6): 29-33. [in Chinese])
马光恕,刘 涛,廉 华,等.2013. 氮钾配施对麦瓶草(面条菜)品质性状及相关酶活性的影响. 中国土壤与肥料,(4): 77-82.(Ma G S, Liu T, Lian H, et al. 2013. Effects of combined application of nitrogen and potassium on quality traits and related enzymes activity of Silene conoidea L. (noodles greens). Soil and Fertilizer Sciences in China,(4): 77-82. [in Chinese])
潘超美, 郭庆荣, 邱桥姐, 等. 2000. VAM 菌根真菌对玉米生长及根际土壤微生态环境的影响. 土壤与环境, 9(4): 304-306.(Pang C M, Guo Q R, Qiu Q J, et al. 2000. Effect of VAM fungus on the growth of core and micro-ecological environment of core rhizosphere. Soil and Environmental Sciences, 9(4): 304-306. [in Chinese])
秦芳玲, 田中民. 2009. 同时接种解磷细菌与丛枝菌根真菌对低磷土壤红三叶草养分利用的影响. 西北农林科技大学学报:自然科学版, 37(6): 151-157.(Qin F L, Tian Z M. 2009. Effect of co-inoculation with arbuscular mycorrhizal fungi and four different phosphate-solubilizing bacteria on nutrients uptake of red clover in a low phosphorus soil. Journal of Northwest A&F University:Natural Science Edition, 37(6): 151-157. [in Chinese])
任嘉红, 李 浩, 刘 辉, 等. 2016. 吡咯伯克霍尔德氏菌JK-SH007对杨树根际微生物数量及功能多样性的影响. 林业科学, 52(5): 126-133.(Ren J H, Li H, Liu H, et al. 2016. Influence of Burkholderia pyrrocinia JK-SH007 on the microbial population and functional diversity of microbial communities in the rhizosphere soil of poplar. Scientia Silvae Sinicae, 52(5): 126-133. [in Chinese])
盛江梅, 吴小芹, 侯亮亮.2014.黑松—黄色须腹菌共生体根际菌根辅助细菌的筛选及鉴定. 东北林业大学学报, 42(5): 110-114.(Sheng J M, Wu X Q, Hou L L. 2014. Isolating and identifying mycorrhiza helper bacteria from the rhizosphere soil of Pinus thunbergii inoculated with Rhizipogen luteous. Journal of Northeast Forestry University, 42(5): 110-114. [in Chinese])
宋 微, 吴小芹. 2011. 外生菌根真菌对NL-895杨光合作用的影响. 西北植物学报, 31(7): 1474-1478.(Song W, Wu X Q. 2011. Effect of ectomycorrhizal fungi on photosynthesis of poplar NL-895. Acta Botanica Boreali-Occidentalia Sinica, 31(7): 1474-1478. [in Chinese])
唐 菁. 2006. 杨树施用细菌肥料的增长效应及作用机理研究. 北京:中国林业科学研究院博士论文.(Tang J. 2006. Studies on promotion of bacterial fertilizers on poplar growth and its mechanism. Beijing: PhD thesis of Chinese Academy of Forestry. [in Chinese])
姚如斌,吴小芹. 2012. 高效解磷细菌与菌根真菌菌剂交互作用对杨树的促生效应. 南京林业大学学报:自然科学版, 36(5): 170-173.(Yao R B, Wu X Q. 2012. Interaction between high effective phosphate-solubilizing bacteria and mycorrhizal fungi and its effects on poplar growth. Journal of Nanjing Forestry University:Natural Science Edition, 36(5): 170-173. [in Chinese])
曾丽琼. 2010. 几种优良外生菌根菌的应用和胶丸菌剂的制备. 南京:南京林业大学硕士论文.(Zeng L Q. 2010. Field application of ectomcorrhizal fungi and study on the process of capsules microbial agent. Nanjing: MS thesis of Nanjing Forestry University. [in Chinese])
赵秀云,韩素芬. 2001. 固氮细菌与杨树的联合固氮作用. 南京林业大学学报:自然科学版, 25(4): 17-20.(Zhao X Y, Han S F. 2001. Nitrogen fixing bacteria and poplar’s associative nitrogen fixation. Journal of Nanjing Forestry University:Natural Science Edition, 25(4): 17-20.[in Chinese])
Adesemoye A O, Torbert H A, Kloepper J W. 2008. Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Canadian Journal of Microbiology, 54: 876-886.
Aspray T J, Frey-Klett P, Jones J E. 2006. Mycorrhization helper bacteria: a case of specificity for altering ectomycorrhiza architecture but not ectomycorrhiza formation. Mycorrhiza, 16(8): 533-541.
Duponnois R, Plenchette C. 2003. A mycorrhiza helper bacterium enhances ectomycorrhizal and endomycorrhizal symbiosis of Australian Acacia species. Mycorrhiza, 13(2): 85-91.
Founoune H, Duponnois R, Meyer J M, et al. 2002. Interactions between ectomycorrhizal symbiosis and fluorescent pseudomonads on Acacia holosericea: isolation of mycorrhiza helper bacteria (MHB) from a Soudano-Sahelian soil. FEMS Microbiology Ecology, 41(1): 37-46.
Hassani F, Ardakani M, Asgharzade A, et al. 2014. Efficiency of mycorrhizal fungi and phosphate solubilizing bacteria on phosphorus uptake and chlorophyll index in potato plantlets. International Journal of Biosciences, 4(1): 244-251.
Kim K Y, Jordan D. McDonald G.A. 1998. Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity. Biology and Fertility of Soils, 26: 79-87.
Liu H, Wu X Q, Ren J H. and Ye J R. 2011. Isolation and identification of phosphobacteria in poplar rhizosphere rom different regions of China. Pedosphere, 21(1): 90-97.
Nadeem S M, Ahmad M, Zahir Z, et al. 2014. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnology Advances, 32(2): 429-448.
Zaidi A, Khan M S. 2007. Stimulatory effects of dual inoculation with phosphate-solubilizing microorganisms and arbuscular mycorrhizal fungus on chickpea. Australian Journal of Experimental Agriculture, 47(8): 1016-1022.