菌根辅助细菌短小芽孢杆菌HR10扩繁条件优化
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摘要
菌根辅助细菌(mycorrhiza helper bacteria,MHB)短小芽孢杆菌(Bacillus pumilus)HR10可与外生菌根真菌(ectomycorrhizal fungi,EMF)互作,对马尾松具有显著促生作用。为将短小芽孢杆菌HR10进行规模化应用,在前期对其增殖扩繁培养基成分优化的基础上,对短小芽孢杆菌HR10培养条件(p H、发酵瓶装液量、发酵温度、接种量)进行了试验优化。结果表明,该菌株最适扩繁条件为:p H 6.5、装液量50%、发酵温度28℃、接种量5%。发酵液生物量在此条件下比前期的培养条件下提高了10.4%。
Previous research indicated that Bacillus pumilus HR10 could significantly promote the growth of Pinus massoniana in association with ectomycorrhizal fungi. With the purpose of expanding the application of this strain,the fermentation conditions of B. pumilus HR10(p H,fermentation broth,temperature and inoculation amount) were optimized based on our previous culture medium optimization. Our results suggested that the optimal fermentation conditions for the propagation of B. pumilus HR10 were p H 6. 5,fermentation broth 50%,temperature 28 ℃,inoculation amount 5%. The biomass in the fermentation broth showed an increase of 10. 4% comparing with original fermentation conditions.
Previous research indicated that Bacillus pumilus HR10 could significantly promote the growth of Pinus massoniana in association with ectomycorrhizal fungi. With the purpose of expanding the application of this strain,the fermentation conditions of B. pumilus HR10(p H,fermentation broth,temperature and inoculation amount) were optimized based on our previous culture medium optimization. Our results suggested that the optimal fermentation conditions for the propagation of B. pumilus HR10 were p H 6. 5,fermentation broth 50%,temperature 28 ℃,inoculation amount 5%. The biomass in the fermentation broth showed an increase of 10. 4% comparing with original fermentation conditions.
引文
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[2]Hassen A I,Labuschagne N.Root colonization and growth enhancement in wheat and tomato by rhizobacteria isolated from the rhizoplane of grasses[J].World J Microbiol Biotechnol,2010,26(10):1837-1846.
[3]盛江梅.黑松菌根根际菌根辅助细菌(MHB)筛选研究[D].南京:南京林业大学,2008.
[4]侯亮亮.黑松根际菌根辅助细菌与外生菌根真菌互作及其促生机制研究[D].南京:南京林业大学,2009.
[5]黄谚谚,吴华珠,许旭萍.短小芽孢杆菌产碱性纤维素酶的发酵工艺优化[J].贵州农业科学,2012,40(5):172-175.
[6]龚勇良,朱传合,刘丽英.短小芽孢杆菌产角蛋白酶液体发酵条件的优化[J].食品与发酵科技,2014,50(1):19-24.
[7]林司曦,吴小芹,丁晓磊,等.短小芽孢杆菌Bacillus pumilus HR10增殖扩繁培养基组分优化[J].微生物学杂志,2014,34(6):32-38.
[8]张蕾,石新丽,李敏,等.短小芽孢杆菌TY079产抗菌物质的发酵培养基研究[J].河北省科学院学报,2010,27(2):47-50.
[9]胡婷婷.油菜籽皮发酵纤维素酶菌种的选育及条件优化[D].武汉:湖北工业大学,2008,40-42.
[10]林运萍.枯草芽孢杆菌B68发酵条件及诱变选育的研究[D].广州:华南热带农业大学,2006.
[11]谢卉.产琼胶酶菌株Agarivorans albus QM38的发酵条件优化、酶的分离纯化及酶学性质研究[D].青岛:中国海洋大学,2009.
[12]张智,黄放,王闯.青春双歧杆菌耐氧驯化及培养条件的响应面法优化[J].食品工业科技,2011,32(4):208-212.
[13]谷祖敏,纪明山,韩秀华,等.鸭跖草叶点霉粗毒素的提取方法和产毒条件的研究[J].微生物学通报,2007,34(5):946-949.
[14]荆卓琼,李美丽,陈秀蓉,等.枯草芽孢杆菌B2发酵工艺及其质量检测方法研究[J].甘肃农业大学学报,2010,4(2):121-142.