HPLC测定青萘伯喹片中萘酚喹的含量
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摘要
目的:建立高效液相色谱法测定青萘伯喹片中萘酚喹含量。方法:采用Diamonsil ODS C18柱(4.6 mm×250mm,5μm);乙腈-0.25%二乙胺溶液(磷酸调节pH至2.5)(23∶77)为流动相,流速0.8 mL.min-1,检测波长为342 nm,柱温25℃。结果:萘酚喹在0.16～0.8 mg.L-1与峰面积呈良好的线性关系(r=0.999 976,n=5),平均加样回收率为99.56%,RSD 0.32%(n=9)。结论:该法操作简便、准确,适用于复方制剂青萘伯喹片中萘酚喹的含量测定。
Objective:To establish an HPLC method for the determinetion of naphthoquine in antimalaria drug Artemisinine Naphthoquine Primaquine Tablets.Method:The assay was performed by a Diamonsil ODS C18 columm(4.6 mm×250 mm,5 μm)and a mobile phase of acetonitrile-0.25% diethylamine with radio of 23 ∶ 77(pH 2.5,adjusted with phosphoric acid),and the flow rate was 0.8 mL · min-1.The wavelength of detector was set at 342 nm and the column temperature was maintained at 25 ℃.Result: Naphthoquine has a good linearity in the rang of 0.16-0.8 μg(r=0.999 9),and the average recovery was 99.56%with RSD of 0.32%(n=9).Conclusion:The method was simple,accurate and suitable for the determination of naphtoquine in Artemisinine Naphthoquine Primaquine Tablets.
Objective:To establish an HPLC method for the determinetion of naphthoquine in antimalaria drug Artemisinine Naphthoquine Primaquine Tablets.Method:The assay was performed by a Diamonsil ODS C18 columm(4.6 mm×250 mm,5 μm)and a mobile phase of acetonitrile-0.25% diethylamine with radio of 23 ∶ 77(pH 2.5,adjusted with phosphoric acid),and the flow rate was 0.8 mL · min-1.The wavelength of detector was set at 342 nm and the column temperature was maintained at 25 ℃.Result: Naphthoquine has a good linearity in the rang of 0.16-0.8 μg(r=0.999 9),and the average recovery was 99.56%with RSD of 0.32%(n=9).Conclusion:The method was simple,accurate and suitable for the determination of naphtoquine in Artemisinine Naphthoquine Primaquine Tablets.
引文
[1]中华人民共和国卫生部部颁标准(暂行)[S].磷酸萘酚喹质量标准(WS-84(X-67)-931993).
[2]杨家庆,詹利之,林燕芳,等.非水溶液滴定法测定抗药萘酚喹的含量[J].中国实验方剂学杂志,2012,18(5):104.
[3]张玉英,潘锡强.HPLC法同时测定复方萘酚喹片中磷酸萘酚喹和甲氧苄啶的含量[J].中国现代应用药学杂志,2000,17(6):472.
[4]彭茗,宋冬梅,杨永健,等.RP-HPLC法测定磷酸萘酚喹的有关物质及含量[J]中国新药杂志,2010,19(10):897.
[5]李鑫健,方卒芬,祝明.HPLC法同时测定利胆排石片中大黄素、大黄酚、及大黄素甲醚[J].中国实验方剂学杂志,2011,17(23):82.
[6]杨青,郭炎荣.RP-HPLC法测定盐酸洛美沙星滴眼液的含量[J].中国实验方剂学杂志,2009,15(4):24.
[2]杨家庆,詹利之,林燕芳,等.非水溶液滴定法测定抗药萘酚喹的含量[J].中国实验方剂学杂志,2012,18(5):104.
[3]张玉英,潘锡强.HPLC法同时测定复方萘酚喹片中磷酸萘酚喹和甲氧苄啶的含量[J].中国现代应用药学杂志,2000,17(6):472.
[4]彭茗,宋冬梅,杨永健,等.RP-HPLC法测定磷酸萘酚喹的有关物质及含量[J]中国新药杂志,2010,19(10):897.
[5]李鑫健,方卒芬,祝明.HPLC法同时测定利胆排石片中大黄素、大黄酚、及大黄素甲醚[J].中国实验方剂学杂志,2011,17(23):82.
[6]杨青,郭炎荣.RP-HPLC法测定盐酸洛美沙星滴眼液的含量[J].中国实验方剂学杂志,2009,15(4):24.