摘要
以茎瘤芥叶为试材,经过烘干、粉碎和灭酶处理,选取甲醇浓度、料液比、提取温度和提取时间进行单因素试验和正交试验,研究了茎瘤芥叶芥子油苷的最佳提取工艺。结果表明:茎瘤芥叶粉需在沸水浴中灭酶100 min,提取条件优化为甲醇浓度90%,料液比1:10,70℃的恒水浴中提取15 min,振摇洗脱2次,可以达到最佳的提取效果。
After drying,crushing and enzyme inactivation leaves of tumorous stem mustard,choose methanol concentration,solid-liquid ratio,extraction temperature and extraction time for the single factor test and orthogonal test,research the best extraction process of glucosinolate in leaves of tumorous stem mustard.The results showed that the best effect of extraction can be achieved by inactivation in boiling water 100 min,extraction conditions on methanol concentration 90% ,aterial liquid ratio 1:10,and shake 2 times.
引文
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