樟子松乙醇提取物体外抗氧化活性研究
|
摘要
测定了樟子松球果、树皮、树叶、木质部4个部位提取物的总抗氧化能力、总还原力、二苯代苦味酰基(DPPH·)清除能力、羟基自由基(·OH)清除能力、超氧阴离子■清除能力5个指标,并以抗坏血酸(Vc)为阳性对照评价从樟子松4个部位提取的松多酚体外抗氧化活性。结果表明:(1)从樟子松树皮中提取的樟子松多酚表现出较强的抗氧化能力,在较高浓度下与抗氧化剂(Vc)相差较小;从樟子松球果中提取的樟子松多酚也表现出较强的还原力,甚至超过阳性对照抗氧化剂Vc;(2)从樟子松树皮中提取的樟子松多酚在二苯代苦味酰基(DPPH·)清除能力、羟基自由基(·OH)清除能力上均为三者之中最高的,但都不超过抗氧化剂(Vc)的抗氧化能;(3)超氧阴离子■清除能力。三者的清除率均为负值,说明该方法测定超氧阴离子■清除率存在干扰因素,无法比较三者在这一指标上的能力大小。上述结果表明樟子松乙醇提取物具有较强的抗氧化能力,可作为天然的抗氧化剂。
The ethanol extractantioxidant activity of cone,bark,leaf,xylem four parts of Pinus sylvestris var.mongolica was compared to provide theoretical basis for further exploitation and utilization of pine resources.P. sylvestris var. mongolica bark,leaves,cones,xylem four parts as raw material,the content of polyphenols and flavonoids in the ethanol extract of camphor was determined. Determination of the P. sylvestris var.mongolica ethanol extracts of polyphenols,flavonoids,and ascorbic acid( VC) is applied to make positive control evaluation of the extracts from different parts of total antioxidant capacity,total reducing power,diphenyl bitter acyl( DPPH·) cleaning ability,hydroxyl free radical( ·OH) clear,super oxygen anion■ remove the five indicators,evaluation of pine ethanol extractpolyphenols ofmongolica four parts in vitro antioxidant activity,draw standard curve. The results showed that:( 1) mongolica polyphenols extracted from P. sylvestris var. mongolica bark showed strong antioxidant capacity,in relatively high concentrations and antioxidants( Vc)is smaller; The camphor pine polyphenols,extracted from camphor cones,also exhibit stronger restorative powers,even more than antioxidants( Vc);( 2) P. sylvestris var. mongolica polyphenols extracted from which bark in diphenyl bitter acyl( DPPH·) cleaning ability,hydroxyl free radical( ·OH) removal ability are the highest of the three,but no more than antioxidants( Vc) antioxidant capacity;( 3) super oxygen anion■cleaning ability index,the clearance of all negative,shows that the method for determining the ultra oxygen anion■ the clearance interference factors are unable to compare three size on this metric capability.Experimental results show that the polyphenols have strong antioxidant ability,which can be used as a natural antioxidant.
The ethanol extractantioxidant activity of cone,bark,leaf,xylem four parts of Pinus sylvestris var.mongolica was compared to provide theoretical basis for further exploitation and utilization of pine resources.P. sylvestris var. mongolica bark,leaves,cones,xylem four parts as raw material,the content of polyphenols and flavonoids in the ethanol extract of camphor was determined. Determination of the P. sylvestris var.mongolica ethanol extracts of polyphenols,flavonoids,and ascorbic acid( VC) is applied to make positive control evaluation of the extracts from different parts of total antioxidant capacity,total reducing power,diphenyl bitter acyl( DPPH·) cleaning ability,hydroxyl free radical( ·OH) clear,super oxygen anion■ remove the five indicators,evaluation of pine ethanol extractpolyphenols ofmongolica four parts in vitro antioxidant activity,draw standard curve. The results showed that:( 1) mongolica polyphenols extracted from P. sylvestris var. mongolica bark showed strong antioxidant capacity,in relatively high concentrations and antioxidants( Vc)is smaller; The camphor pine polyphenols,extracted from camphor cones,also exhibit stronger restorative powers,even more than antioxidants( Vc);( 2) P. sylvestris var. mongolica polyphenols extracted from which bark in diphenyl bitter acyl( DPPH·) cleaning ability,hydroxyl free radical( ·OH) removal ability are the highest of the three,but no more than antioxidants( Vc) antioxidant capacity;( 3) super oxygen anion■cleaning ability index,the clearance of all negative,shows that the method for determining the ultra oxygen anion■ the clearance interference factors are unable to compare three size on this metric capability.Experimental results show that the polyphenols have strong antioxidant ability,which can be used as a natural antioxidant.
引文
1.喻泓.呼伦贝尔沙地樟子松林对林火干扰的响应[D].北京:中国林业科学研究院,2009.YU H.Responses of Mongolian pine forest to wildland fire in the Hulun Buir sandland,Inner Mongolia,China[D].Beijing:Chinese Academy of Forestry Sciences,2009.
2.李波,包怡红,王振宇.松多酚生物活性研究进展[J].黑龙江农业科学,2013(10):133-136.Li B,Bao Y H,Wang Z Y.Research progress on biological activity of pine polyphenol[J].Heilongjiang Agricultural Sciences,2013(10):133-136.
3.Liazid A,Schwarz M,Varela R M,et al.Evaluation of various extractiontechniques for obtaining bioactive extracts from pine seeds[J].Foodand Bioproducts Processing,2010,88(2-3):247-252.
4.Gordon M H.Dietary antioxidants in disease prevention[J].Natural Product Reports,1996,13(4):265-273.
5.Rohdewald P.A review of the French maritime pine bark extract(Pycnogenol),a herbal medication with a diverse clinical pharmacology[J].International Journal of Clinical Pharmacology and Therapeutics,2002,40(4):158-168.
6.刘冉,王振宇,卢静,等.两种松科植物松多酚体外抗氧化活性评价[J].食品工业科技,2013,34(4):159-163.Liu R,Wang Z Y,Lu J,et,al.Analysis of antioxidant properties of pine polyphenols fromtwo species of Pinaceae in vitro[J].Science and Technology of Food Industry,2013,34(4):159-163.
7.Pinelo M,Rubilar M,Sineiro J,etal.Extraction of antioxidantphenolics from almond hulls(Prunus amygdalus)and pine sawdust(Pinus pinaster)[J].Food Chemistry,2004,85(2):267-273.
8.Yu L M,Zhao M M,Wang J S,et al.Antioxidant,immunomodulatory and anti-breast cancer activities of phenolic extract frompine(Pinus massonlana Lamb)bark[J].Innovative Food Science&Emerging Technologies,2008,9(1):122-128.
9.郭庆启,张娜,李梦云,等.落叶松松塔多酚成分和抗氧化能力与海拔的相关性研究[J].北京林业大学学报,2013,35(1):59-63.Guo Q Q,Zhang N,Li M Y,etal.Relationship betweenpolyphenols and antioxidation in larch pine cones and altitude[J].Journal of Beijing Forestry University,2013,35(1):59-63.
10.Taner G,Aydin S,Ayta9 Z,et al.Genotoxic and antigenotoxic effects of pycnogenol in human lymphocytes assessed by the alkaline comet assay[J].Toxicology Letters,2011,S205:S111.
11.李珊珊,吴倩,袁茹玉,等.莲属植物类黄酮代谢产物的研究进展[J].植物学报,2014,49(6):738-750.Li S S,Wu Q,Yuan R Y,etal.Recent advances in metabolic products of flavonoids in Nelumbo[J].Chinese Bulletin of Botany,2014,49(6):738-750.
12.陈小红,韦莉,黄玉琼,等.鸳鸯茉莉花色变化过程中的生理生化特性研究[J].植物科学学报,2018,36(4):595-602.Chen X H,Wei L,Huang Y Q,et al.Study on the physiological and biochemical characteristics of Brunfelsia acuminata petals during flowering[J].Plant Science Journal,2018,36(4):595-602.
13.刘素军,蒙美莲,陈有君.干旱胁迫及复水对马铃薯类黄酮合成途径中关键酶及基因表达的影响[J].植物生理学报,2018,54(1):81-91.Liu S J,Meng M L,Chen Y J.Effects of drought stress and rehydration on gene expression of key enzymes in the flavonoid biosynthesis pathway in potato[J].Plant Physiology Journal,2018,54(1):81-91.
14.蒋明义.水分胁迫下植物体内·OH的产生与细胞的氧化损伤[J].植物学报,1999,41(3):229-234.Jiang M Y.Generation of hydroxyl radicals and its relation to cellular oxidative damage in plants subjected to water stress[J].Acta Botanica Sinica,1999,41(3):229-234.
15.景红娟,周广舟,谭晓荣,等.活性氧对植物自噬调控的研究进展[J].植物学报,2012,47(5):534-542.Jing H J,Zhou G Z,Tan X R,etal.Research progress in regulation of reactive oxygen species in plant autophagy[J].Chinese Bulletin of Botany,2012,47(5):534-542.
16.张梦如,杨玉梅,成蕴秀,等.植物活性氧的产生及其作用和危害[J].西北植物学报,2014,34(9):1916-1926.Zhang M R,Yang Y M,Cheng Y X,etal.Generation of reactive oxygen species and their functions and deleterious effects in plants[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(9):1916-1926.
17.张力平,孙长霞,李俊清,等.植物多酚的研究现状及发展前景[J].林业科学,2005,41(6):157-162.Zhang L P,Sun C X,Li J Q,etal.The present conditions and development trend of plant polyphenols research[J].Scientia Silvae Sinicae,2005,41(6):157-162.
18.范现丽,王高,王宏,等.香桃木叶片粗多酚抗氧化活性的研究[J].植物研究,2009,29(3):375-379.Fan X L,Wang G,Wang H,etal.Antioxidant activities of crude polyphenol extracts from leaves of Myrtus communis[J].Bulletin of Botanical Research,2009,29(3):375-379.
2.李波,包怡红,王振宇.松多酚生物活性研究进展[J].黑龙江农业科学,2013(10):133-136.Li B,Bao Y H,Wang Z Y.Research progress on biological activity of pine polyphenol[J].Heilongjiang Agricultural Sciences,2013(10):133-136.
3.Liazid A,Schwarz M,Varela R M,et al.Evaluation of various extractiontechniques for obtaining bioactive extracts from pine seeds[J].Foodand Bioproducts Processing,2010,88(2-3):247-252.
4.Gordon M H.Dietary antioxidants in disease prevention[J].Natural Product Reports,1996,13(4):265-273.
5.Rohdewald P.A review of the French maritime pine bark extract(Pycnogenol),a herbal medication with a diverse clinical pharmacology[J].International Journal of Clinical Pharmacology and Therapeutics,2002,40(4):158-168.
6.刘冉,王振宇,卢静,等.两种松科植物松多酚体外抗氧化活性评价[J].食品工业科技,2013,34(4):159-163.Liu R,Wang Z Y,Lu J,et,al.Analysis of antioxidant properties of pine polyphenols fromtwo species of Pinaceae in vitro[J].Science and Technology of Food Industry,2013,34(4):159-163.
7.Pinelo M,Rubilar M,Sineiro J,etal.Extraction of antioxidantphenolics from almond hulls(Prunus amygdalus)and pine sawdust(Pinus pinaster)[J].Food Chemistry,2004,85(2):267-273.
8.Yu L M,Zhao M M,Wang J S,et al.Antioxidant,immunomodulatory and anti-breast cancer activities of phenolic extract frompine(Pinus massonlana Lamb)bark[J].Innovative Food Science&Emerging Technologies,2008,9(1):122-128.
9.郭庆启,张娜,李梦云,等.落叶松松塔多酚成分和抗氧化能力与海拔的相关性研究[J].北京林业大学学报,2013,35(1):59-63.Guo Q Q,Zhang N,Li M Y,etal.Relationship betweenpolyphenols and antioxidation in larch pine cones and altitude[J].Journal of Beijing Forestry University,2013,35(1):59-63.
10.Taner G,Aydin S,Ayta9 Z,et al.Genotoxic and antigenotoxic effects of pycnogenol in human lymphocytes assessed by the alkaline comet assay[J].Toxicology Letters,2011,S205:S111.
11.李珊珊,吴倩,袁茹玉,等.莲属植物类黄酮代谢产物的研究进展[J].植物学报,2014,49(6):738-750.Li S S,Wu Q,Yuan R Y,etal.Recent advances in metabolic products of flavonoids in Nelumbo[J].Chinese Bulletin of Botany,2014,49(6):738-750.
12.陈小红,韦莉,黄玉琼,等.鸳鸯茉莉花色变化过程中的生理生化特性研究[J].植物科学学报,2018,36(4):595-602.Chen X H,Wei L,Huang Y Q,et al.Study on the physiological and biochemical characteristics of Brunfelsia acuminata petals during flowering[J].Plant Science Journal,2018,36(4):595-602.
13.刘素军,蒙美莲,陈有君.干旱胁迫及复水对马铃薯类黄酮合成途径中关键酶及基因表达的影响[J].植物生理学报,2018,54(1):81-91.Liu S J,Meng M L,Chen Y J.Effects of drought stress and rehydration on gene expression of key enzymes in the flavonoid biosynthesis pathway in potato[J].Plant Physiology Journal,2018,54(1):81-91.
14.蒋明义.水分胁迫下植物体内·OH的产生与细胞的氧化损伤[J].植物学报,1999,41(3):229-234.Jiang M Y.Generation of hydroxyl radicals and its relation to cellular oxidative damage in plants subjected to water stress[J].Acta Botanica Sinica,1999,41(3):229-234.
15.景红娟,周广舟,谭晓荣,等.活性氧对植物自噬调控的研究进展[J].植物学报,2012,47(5):534-542.Jing H J,Zhou G Z,Tan X R,etal.Research progress in regulation of reactive oxygen species in plant autophagy[J].Chinese Bulletin of Botany,2012,47(5):534-542.
16.张梦如,杨玉梅,成蕴秀,等.植物活性氧的产生及其作用和危害[J].西北植物学报,2014,34(9):1916-1926.Zhang M R,Yang Y M,Cheng Y X,etal.Generation of reactive oxygen species and their functions and deleterious effects in plants[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(9):1916-1926.
17.张力平,孙长霞,李俊清,等.植物多酚的研究现状及发展前景[J].林业科学,2005,41(6):157-162.Zhang L P,Sun C X,Li J Q,etal.The present conditions and development trend of plant polyphenols research[J].Scientia Silvae Sinicae,2005,41(6):157-162.
18.范现丽,王高,王宏,等.香桃木叶片粗多酚抗氧化活性的研究[J].植物研究,2009,29(3):375-379.Fan X L,Wang G,Wang H,etal.Antioxidant activities of crude polyphenol extracts from leaves of Myrtus communis[J].Bulletin of Botanical Research,2009,29(3):375-379.