两种速冻机速冻效果的对比分析
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摘要
目的比较两种不同速冻方式的速冻机,选择更适合的速冻方式,缩短血浆速冻时间,提高速冻效果。方法200mL新鲜血浆25袋,称重平均分成2袋并标记,分别置于MBF-21平板血浆速冻机和YCS-I对流型血液成分速冻机内进行速冻,再经过解冻融化留样检测FⅧ、Fbg含量。利用温度探头模拟袋监测两台速冻机速冻温度的变化情况。结果同源、同量的新鲜冰冻血浆中的FⅧ含量,以平板速冻机明显高于强制空气对流型速冻机(P <0.01);Fbg含量比较差异无统计学意义(P>0.05)。相对于强制对流型速冻机,平板速冻机温度下降速度快。结论两种速冻机的速冻效果存在差异,平板速冻机要优于强制空气对流型速冻机。
Objective To compare the two quick-freezing freezers in plasma storage,shorten freezing time of plasma and improve the freezing effect. Method Twenty five bags of 200 mL fresh plasma were weighed and evenly divided into two bags with labels,synchronously placed in the MBF-2 1 plate plasma machine and the YCS-I convection blood component machine,and then thawed to detect the contents of FⅧ and Fbg. The temperature probe simulating bag was used to monitor the quick freezing temperature changes of the two freezers. Result The content of FⅧ in freshly frozen plasma of the same amount was significantly higher than that in forced air convection freezer(P <0.01). There was no significant difference in Fbg content(P >0.05). Compared to the forced convection freezer,flat-panel freezer temperature dropped faster. Conclusion The flat-plate quick-freezing machine is superior to the forced air convection type quick-freezing machine in fresh plasma freezing.
Objective To compare the two quick-freezing freezers in plasma storage,shorten freezing time of plasma and improve the freezing effect. Method Twenty five bags of 200 mL fresh plasma were weighed and evenly divided into two bags with labels,synchronously placed in the MBF-2 1 plate plasma machine and the YCS-I convection blood component machine,and then thawed to detect the contents of FⅧ and Fbg. The temperature probe simulating bag was used to monitor the quick freezing temperature changes of the two freezers. Result The content of FⅧ in freshly frozen plasma of the same amount was significantly higher than that in forced air convection freezer(P <0.01). There was no significant difference in Fbg content(P >0.05). Compared to the forced convection freezer,flat-panel freezer temperature dropped faster. Conclusion The flat-plate quick-freezing machine is superior to the forced air convection type quick-freezing machine in fresh plasma freezing.
引文
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6王憬惺,主编·输血技术[M].第3版,北京:人民卫生出版社,2013:85-88.
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8付涌水,主编.临床输血[M].第3版.北京:人民卫生出版社,2013:40-43.
9李建华,周忠英,张瑜.2种速冻方式对新鲜冰冻血浆和冷沉淀质量的影响比较[J].中国输血杂志,2015,28(8):979-980.
10傅国英,徐雪梅,江素君.速冻设备与速冻时间对制备冷沉淀质量的探讨[J].中国输血杂志,2017,30(7):836-837.
11李阿中,周华平.2种血浆速冻方法的比较[J].中国输血杂志.2013,26(9):913-914.
2赵宏祥,葛健民,程玉根.新鲜冰冻血浆制备中凝血因子活性保护的探讨[J].中国现代医生,2007,45(2):16-17.
3何红.延长制备时间对新鲜冰冻血浆和冷沉淀质量的影响[J].血栓与止血学,2016,22(1):79-81.
4中华人民共和国卫生部.全血及成分血质量要求(GB1849-2012). 2012:07.
5刘晓丽,张微,徐书琴,等.三种速冻方法制备新鲜冰冻血浆的比较分析[J].中国输血杂志[J].2016,29(1):85-87.
6王憬惺,主编·输血技术[M].第3版,北京:人民卫生出版社,2013:85-88.
7 Serious hazards of transfusion(SHOT).Annual Report2003. Manchester:Serious Hazards of Transfusion Steering Group, 2003.
8付涌水,主编.临床输血[M].第3版.北京:人民卫生出版社,2013:40-43.
9李建华,周忠英,张瑜.2种速冻方式对新鲜冰冻血浆和冷沉淀质量的影响比较[J].中国输血杂志,2015,28(8):979-980.
10傅国英,徐雪梅,江素君.速冻设备与速冻时间对制备冷沉淀质量的探讨[J].中国输血杂志,2017,30(7):836-837.
11李阿中,周华平.2种血浆速冻方法的比较[J].中国输血杂志.2013,26(9):913-914.