用纳氏试剂法测定氨氮时校准曲线的时效性研究
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摘要
采用纳氏试剂分光光度法测定氨氮,在主要试剂配制好后绘制了一条参考校准曲线。分别在2周、1个月、2个月、3个月后绘制校准曲线,对当前样品检测结果与参考校准曲线代入计算的结果进行对比。结果表明,在主要试剂未重新配制的情况下,用参考校准曲线计算的结果与各时期样品检测前绘制校准曲线的计算结果无显著差异。为减少校准曲线的绘制次数,可用参考校准曲线直接代入计算样品氨氮浓度。
A reference calibration curve was drawn after the main reagent was prepared during the determination of ammonia nitrogen concentration by Nessler's reagent spectrophotometer method. The calibration curves were drawn after two weeks, one month, two months and three months, respectively. The ammonia nitrogen concentrations of the current samples were compared with those calculated by the reference calibration curves. The results showed that there was no significant difference between the ammonia nitrogen concentrations calculated by reference calibration curves and those calculated by the calibration curves drawn at different periods, when the main reagents were not re-prepared. Thus, the reference calibration curve can be directly used to calculate the sample's concentration in order to reduce the number of times to draw calibration curves.
A reference calibration curve was drawn after the main reagent was prepared during the determination of ammonia nitrogen concentration by Nessler's reagent spectrophotometer method. The calibration curves were drawn after two weeks, one month, two months and three months, respectively. The ammonia nitrogen concentrations of the current samples were compared with those calculated by the reference calibration curves. The results showed that there was no significant difference between the ammonia nitrogen concentrations calculated by reference calibration curves and those calculated by the calibration curves drawn at different periods, when the main reagents were not re-prepared. Thus, the reference calibration curve can be directly used to calculate the sample's concentration in order to reduce the number of times to draw calibration curves.
引文
[1]纪昳,孙娟,乔丹丹等.纳氏试剂比色法测定污水中氨氮的优化研究[J].环境科学与管理,2017,42(5):123-125.
[2]封跃鹏,邱赫男,孙自杰.纳氏试剂分光光度法测定水中氨氮研究进展[J].环境科学与技术,2016,39(S2):348-352.
[3]邓翔宇.水质监测中氨氮测定的影响因素分析[J].资源节约与环保,2014(4):123-124.
[4]HJ535-2009,水质氨氮的测定纳氏试剂分光光度法[S].
[2]封跃鹏,邱赫男,孙自杰.纳氏试剂分光光度法测定水中氨氮研究进展[J].环境科学与技术,2016,39(S2):348-352.
[3]邓翔宇.水质监测中氨氮测定的影响因素分析[J].资源节约与环保,2014(4):123-124.
[4]HJ535-2009,水质氨氮的测定纳氏试剂分光光度法[S].