北豆根中化学成分及其抗缺氧活性研究
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摘要
采用制备薄层色谱、硅胶柱色谱、ODS柱色谱、D101大孔树脂柱色谱、Sephadex LH-20凝胶柱色谱等色谱分离方法,对北豆根的75%乙醇提取物进行分离纯化,根据化合物的理化性质和波谱数据进行结构鉴定。从北豆根的75%乙醇提取物中分离得到15个化合物,分别鉴定为N-methylcorydaldine(1)、白蓬草福林(2)、千金藤啶碱(3)、阿克吐明(4)、蝙蝠葛苏林碱(5)、阿克吐米定(6)、蝙蝠葛新苛林碱(7)、蝙蝠葛辛碱(8)、6-O-去甲基蝙蝠葛波酚碱(9)、蝙蝠葛定碱(10)、蝙蝠葛糖苷(11)、刺五加苷D(12)、马兜铃内酯(13)、马兜铃酸萜酯Ⅰ(14)和马兜铃酸(15),化合物12～15为首次从蝙蝠葛属植物中分离得到。利用MTT法对所分得量较大的6种生物碱进行了抗缺氧活性研究,结果表明6种生物碱均具有较显著的抗缺氧活性。
To study the chemical constituents from the rhizome of Menispermum dauricum,fifteen compounds,N-methylcorydaldine( 1),thalifoline( 2),stepholidine( 3),acutumine( 4),daurisoline( 5),acutumidine( 6),dauricicoline( 7),bianfugecine( 8),6-O-demethylmenisporphine( 9),bianfugedine( 10),dauricoside( 11),eleutheroside D( 12),aristolactone( 13),aristoloterpenateⅠ( 14) and aristolochic acid( 15) were isolated from 75% ethanol extract of Menispermi Rhizoma by using thin layer chromatography and column chromatography methods. Their structures were identified based on their physicochemical properties and spectral data. Among them,compounds 12-15 were obtained from the genus Menispermum for the first time. Six alkaloids with higher contents were subjected to evaluate the anti-hypoxic activities by using MTT method. As a result,six alkaloids exhibited significant anti-hypoxia activities.
To study the chemical constituents from the rhizome of Menispermum dauricum,fifteen compounds,N-methylcorydaldine( 1),thalifoline( 2),stepholidine( 3),acutumine( 4),daurisoline( 5),acutumidine( 6),dauricicoline( 7),bianfugecine( 8),6-O-demethylmenisporphine( 9),bianfugedine( 10),dauricoside( 11),eleutheroside D( 12),aristolactone( 13),aristoloterpenateⅠ( 14) and aristolochic acid( 15) were isolated from 75% ethanol extract of Menispermi Rhizoma by using thin layer chromatography and column chromatography methods. Their structures were identified based on their physicochemical properties and spectral data. Among them,compounds 12-15 were obtained from the genus Menispermum for the first time. Six alkaloids with higher contents were subjected to evaluate the anti-hypoxic activities by using MTT method. As a result,six alkaloids exhibited significant anti-hypoxia activities.
引文
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[3]李晓庄,赵慧玲,黄文平,等.UPLC-Q-TOF-MS/MS快速鉴别北豆根中的生物碱类成分[J].中国实验方剂学杂志,2017,23(15):97.
[4] Chen J Y,Xie Y F,Zhou T X,et al. Chemical constituents ofMenispermum dauricum[J]. Chin J Nat Med,2012,10(4):292.
[5] Zhang Z J,Zhang X Q,Ye W C,et al. A new 1,4-phenanthra-quinone from Menispermum dauricum[J]. Nat Prod Res,2004,18(4):301.
[6]郑艳春,崔雅慧,秦婷.北豆根中生物碱类成分的研究[J].中国当代医药,2011,18(13):17.
[7]郑丽娜,罗栋,孙蓉.北豆根不同组分发挥抗炎作用的安全范围研究[J].中国药物警戒,2012,9(7):335.
[8]苏云明,李永强,周媛.蝙蝠葛酚性碱对实验性心肌缺血保护作用的研究[J].中国药师,2002,6(5):326.
[9] Wang F,Qu L,Lv Q,et al. Effect of phenolic alkaloids fromMenispermum dauricum on myocardial-cerebral ischemia/reperfu-sion injury in rabbits[J]. Acta Pharm Sin,2001,22(12):1130.
[10] Zhao J,Lian Y,Lu C F,et al. Inhibitory effects of a bisbenzyli-soquinline alkaloid dauricine on HERG potassium channels[J].J Ethnopharmacol,2012,141(2):685.
[11]孙蓉,王晨.北豆根毒性研究进展[J].中国药物警戒,2009,6(9):546.
[12]张晓琦,叶文才,赵守训.蝙蝠葛中异喹啉酮的分离与鉴定[J].中国药科大学学报,2001,32(2):96.
[13]王恩军,马云保,张雪梅,等.秤钩风中的生物碱成分[J].中国中药杂志,2008,33(21):2503.
[14] Hu S M,Xu S X,Yao X S,et al. Dauricoside,a new glycosidalalkaloid having an inhibitory activity against blood-platelet aggre-gation[J]. Chem Pharm Bull,1993,41(10):1866.
[15] Sugimoto Y,Babiker H A A,Saisho T,et al. Chlorinated alka-loids in Menispermum dauricum DC. root culture[J]. J OrgChem,2001,66(10):3299.
[16]郑锡文,闵知大,赵守训.蝙蝠葛中的新生物碱———蝙蝠葛苏林碱[J].科学通报,1979,24(6):285.
[17]潘锡平,胡崇家,曾繁典.蝙蝠葛中一新双苄基异喹啉生物碱[J].中国药物化学杂志,1999,9(2):123.
[18]谭宁华,赵守训,丁林生,等.氧化异阿朴啡型生物碱[J].中国药科大学学报,1990,21(6):377.
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[22]周艳丽,赵旭,李硕熙,等.北豆根根茎中生物碱类化学成分及其生物活性研究[J].中国现代中药,2018,20(2):163.