转基因棉花对三突花蛛生物学特性和体内酶活的影响
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摘要
目的:研究2种转基因抗虫棉对三突花蛛生物学特性和体内酶活的影响。方法:在人工条件下,采用棉蚜饲喂法,测定转基因抗虫棉对三突花蛛生物学特性变化的影响,观测三突花蛛的捕食功能反应和体内抗氧化酶活性的变化。结果:取食转基因抗虫棉上棉蚜的三突花蛛生物学特性没有显著变化,捕食功能反应表明捕食效率和控虫效果显著下降,体内GST、SOD、CAT酶活成下降趋势,但没有显著差异。结论:转基因抗虫棉对三突花蛛生物学特性和体内酶活无明显不利影响,这为研究转基因棉对棉田捕食天敌的影响补充了基础资料。
Objective:The aim was to study the effects of two kinds of transgenic insect-resistant cotton on the biological characteristics and enzymatic activities of Misumenops tricuspidatus Fabricius.Method:Under artificial conditions,the biological characteristics of Misumenops tricuspidatus Fabricius were measured by feeding cotton aphids from transgenic insect-resistant cotton.And the predatory response and the activity of antioxidant enzymes in vivo were observed.Result:The results showed that there no significant difference in the biological characteristics of T Misumenops tricuspidatus Fabricius feeding on transgenic insectresistant cotton aphids.Predatory functional response showed that the predation efficiency and insect control effect decreased significantly,while the activities of GST,SOD and CAT enzymes decreased in vivo,with no significant difference.Conclusion:Transgenic insect-resistant cotton had no significant adverse effects on the biological characteristics and enzymatic activities of the spider.The research supplements a basic data on the influence of GM cotton on predatory natural enemies in cotton field.
Objective:The aim was to study the effects of two kinds of transgenic insect-resistant cotton on the biological characteristics and enzymatic activities of Misumenops tricuspidatus Fabricius.Method:Under artificial conditions,the biological characteristics of Misumenops tricuspidatus Fabricius were measured by feeding cotton aphids from transgenic insect-resistant cotton.And the predatory response and the activity of antioxidant enzymes in vivo were observed.Result:The results showed that there no significant difference in the biological characteristics of T Misumenops tricuspidatus Fabricius feeding on transgenic insectresistant cotton aphids.Predatory functional response showed that the predation efficiency and insect control effect decreased significantly,while the activities of GST,SOD and CAT enzymes decreased in vivo,with no significant difference.Conclusion:Transgenic insect-resistant cotton had no significant adverse effects on the biological characteristics and enzymatic activities of the spider.The research supplements a basic data on the influence of GM cotton on predatory natural enemies in cotton field.
引文
[1]张立兰,陈亮,张宏福.转基因作物对畜禽肠道非预期效应的研究进展[J].中国农业科技导报,2018,20(11):1-13.
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[12]李笠坤,雒珺瑜,吴益东,等.转Cry1Ac/1Ab基因棉花对棉铃虫的控制效果及对甜菜夜蛾和斜纹夜蛾生长发育的影响[J].植物保护学报,2018,45(3):561-567.
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[14]辛肇军,李照会,叶保华.三突花蛛对亚洲玉米螟幼虫的捕食作用[J].山东农业科学,2012,44(10):89-91.
[15]雒珺瑜,张帅,张开心,等.不同调查方法对转基因棉田节肢动物群落构成及生物多样性的影响[J].生物安全学报,2018,27(2):139-144.
[16]张广铎,张征田,庞振凌,等.取食转基因棉上的白粉虱对T纹豹蛛生长发育及捕食的影响[J].安徽农业科学,2012,40(25):12533-12535.
[17]姜文虎,张德健,刘军侠,等.转Bt基因和非转基因杨树--棉花复合系统中节肢动物群落比较分析[J].林业科学,2018,54(10):73-79.
[18]邓曙东,徐静,张青文,等.转Bt基因棉对非靶标害虫及害虫天敌种群动态的影响[J].昆虫学报,2003,46(1):1-5.
[19]李荣田,王新宇,田崇兵,等.转cry1C*及cry2A*基因早粳稻Bt蛋白的时空表达和抗螟虫性[J].作物学报,2018,44(12):1829-1836.
[20]Han Y,Chen J,Wang H,et al.No impact of transgenic cry2Aa rice on Anagrus nilaparvatae,an egg parasitoid of Nilaparvata lugens,in laboratory tests[J].Biological Control,2015,82:46-51.
[2]崔宁波,郑雪梅.转基因作物产业化风险管理的国际比较及其借鉴[J].江苏农业科学,2018,46(13):1-5.
[3]韩兰芝,白树雄,赵建周,等.转基因抗虫棉花和玉米与节肢动物相关的生态安全性研究进展[J].昆虫学报,2007,50(7):727-736.
[4]ISAAA.Global status of commercialized biotech/GM crops in2017:Biotech crop adoption surges as economic benefits accumulate in 22 years[A]//.In:International Service for the Acquisition of Agribiotech Applications Brief No.53[C].Ithaca New York,2017.
[5]Birkett,M.A.&Pickett,J.A..Aphid sex pheromones:from discovery to commercial production[J].Phytochemistry,2003,62(5):651-656.
[6]王英丽,魏纪珍,张丽丽,等.Cry1Ac和Cry2Ab蛋白对大草蛉生长发育及酶活力的影响[J].植物保护学报,2014,41(3):285-291.
[7]Yu H L,Li Y H,Wu K M.Risk assessment and ecological effects of transgenic Bacillus thuringiensis crops on non-target organisms[J].Journal of Integrative Plant Biology,2011,53(7):520-538.
[8]王福莲,张帆,谢广林.三突花蛛和T纹豹蛛对豇豆荚螟幼虫的捕食作用[J].安徽农业科学,2006,34(2):275-276,278.
[9]邱良妙,刘其全,林仁魁,等.转Bt基因水稻对主要非靶标昆虫的生态安全性研究进展[J].福建农业学报,2018,33(3):326-333.
[10]李海强,王冬梅,李号宾,等.转基因棉花对棉蚜个体生长发育和繁殖能力的影响[J].新疆农业科学,2018,55(8):1467-1472.
[11]王文晶,王瑞林,于福兰,等.转Bt水稻对捕食性天敌黑肩绿盲蝽种群动态的影响[J].应用昆虫学报,2014,51(5):1173-1183.
[12]李笠坤,雒珺瑜,吴益东,等.转Cry1Ac/1Ab基因棉花对棉铃虫的控制效果及对甜菜夜蛾和斜纹夜蛾生长发育的影响[J].植物保护学报,2018,45(3):561-567.
[13]周霞,谢翔,左娇,等.转基因作物对天敌的影响研究进展[J].环境昆虫学报,2018,40(5):1021-1026.
[14]辛肇军,李照会,叶保华.三突花蛛对亚洲玉米螟幼虫的捕食作用[J].山东农业科学,2012,44(10):89-91.
[15]雒珺瑜,张帅,张开心,等.不同调查方法对转基因棉田节肢动物群落构成及生物多样性的影响[J].生物安全学报,2018,27(2):139-144.
[16]张广铎,张征田,庞振凌,等.取食转基因棉上的白粉虱对T纹豹蛛生长发育及捕食的影响[J].安徽农业科学,2012,40(25):12533-12535.
[17]姜文虎,张德健,刘军侠,等.转Bt基因和非转基因杨树--棉花复合系统中节肢动物群落比较分析[J].林业科学,2018,54(10):73-79.
[18]邓曙东,徐静,张青文,等.转Bt基因棉对非靶标害虫及害虫天敌种群动态的影响[J].昆虫学报,2003,46(1):1-5.
[19]李荣田,王新宇,田崇兵,等.转cry1C*及cry2A*基因早粳稻Bt蛋白的时空表达和抗螟虫性[J].作物学报,2018,44(12):1829-1836.
[20]Han Y,Chen J,Wang H,et al.No impact of transgenic cry2Aa rice on Anagrus nilaparvatae,an egg parasitoid of Nilaparvata lugens,in laboratory tests[J].Biological Control,2015,82:46-51.