葛花枳椇子配伍使用对急性酒精中毒小鼠醉酒症状行为学及脑纹状体和海马神经递质的影响
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摘要
目的:探讨葛花枳椇子单独或配伍的最佳给药方式及对急性酒精中毒小鼠脑纹状体多巴胺(dopamine,DA)、5-羟色胺(5-hydroxytryptamine,5-HT)含量和海马乙酰胆碱酯酶(acetylcholinesterase,Ach E)活性的影响。方法:选取ICR小鼠186只,随机分为空白对照组、模型组、阳性对照组、葛花组、枳椇子组及配伍1、2、3组。预防给药,给药30 min后灌酒建立模型,2 h后检测脑纹状体DA、5-HT及海马Ach E的含量。结果:葛花枳椇子单用及配伍对急性酒精中毒的预防作用优于治疗作用。急性酒精中毒小鼠脑纹状体5-HT和海马中Ach E含量较空白对照组显著升高(P<0.05);与模型组比较,枳椇子组可显著降低纹状体DA含量(P<0.05),配伍1,3组可显著降低纹状体5-HT含量(P<0.05)。结论:葛花枳椇子单用及配伍对急性酒精中毒的预防作用更优;葛花枳椇子单独或配伍使用可以调节脑组织神经递质,改善急性酒精中毒时的神经行为异常。
Objective: To investigate better administration method of compatibility of Flos Puerariae Lobatae and Semen Hoveniae,and the influence on content of striatum dopamine( DA),5-hydroxytryptamine( 5-HT) and activity of hippocampus acetylcholinesterase( Ach E). Methods: ICR mice were randomly divided into eight groups,including blank control group,model group,positive control group,Gehua group,Zhijuzi group,and combination group 1,2,3. The mouse model of acute alcoholism was established by giving alcohol to mice orally after 30 min for taking preventive administration method. The striatum DA,5-HT and hippocampus Ach E were measured after 2 h. Results: The compatibility of Flos Puerariae Lobatae and Semen Hoveniae was superior by prevention to treatment. The contents of striatum 5-HT and activity of achippocampus Ach E in the model group were increased compared with those in the blank control group( P < 0. 05). The contents of DA were obviously reduced in Zhijuzi groups( P < 0. 05). The contents of 5-HT were significantly decrease in combination group 1,3( P < 0. 05). Conclusion: There is an improvement on neurobehavioral abnormality by regulating the contents of DA,5-HT and activity of Ach E of compatibility of Flos Puerariae Lobatae and Semen Hoveniae.
Objective: To investigate better administration method of compatibility of Flos Puerariae Lobatae and Semen Hoveniae,and the influence on content of striatum dopamine( DA),5-hydroxytryptamine( 5-HT) and activity of hippocampus acetylcholinesterase( Ach E). Methods: ICR mice were randomly divided into eight groups,including blank control group,model group,positive control group,Gehua group,Zhijuzi group,and combination group 1,2,3. The mouse model of acute alcoholism was established by giving alcohol to mice orally after 30 min for taking preventive administration method. The striatum DA,5-HT and hippocampus Ach E were measured after 2 h. Results: The compatibility of Flos Puerariae Lobatae and Semen Hoveniae was superior by prevention to treatment. The contents of striatum 5-HT and activity of achippocampus Ach E in the model group were increased compared with those in the blank control group( P < 0. 05). The contents of DA were obviously reduced in Zhijuzi groups( P < 0. 05). The contents of 5-HT were significantly decrease in combination group 1,3( P < 0. 05). Conclusion: There is an improvement on neurobehavioral abnormality by regulating the contents of DA,5-HT and activity of Ach E of compatibility of Flos Puerariae Lobatae and Semen Hoveniae.
引文
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[17]刘明,陈绍红,钟赣生,等.葛花枳椇子配伍对酒精性肝损伤大鼠肝脏功能及病理形态的影响[J].南京中医药大学学报,2015,31(2):147-152.
[18]柳海艳,钟赣生,李怡文,等.醇提和水提葛花枳椇子及其配伍对酒精性肝损伤大鼠肝脏抗氧化功能的影响[J].中华中医药杂志,2012,27(4):1181-1184.
[19]潘敏,谢少龙,丘慧秋,等.枳椇子、葛花的最佳配比解酒方影响人体乙醇吸收代谢过程研究[J].中国执业药师,2016,13(3):23-26.
[2]李怡文,刘阳,柳海艳,等.葛花枳椇子配伍使用对醉酒小鼠体内乙醇代谢过程的影响[J].世界中医药,2017,12(8):1885-1889.
[3]张志强,孟欣桐,苗明三.葛花的现代研究与思考[J].中医学报,2016,31(12):1957-1960.
[4]黄菲,潘晓薇,周瑢,等.葛花对缓解小鼠急性酒精中毒的影响[J].烟草科技,2017,50(7):57-61.
[5]宁华.酒精中毒中西药联合治疗的效果观察[J].湖南中医药大学学报,2016,27(a02):1250.
[6]王洁,周瑢,潘晓薇,等.葛根、葛花、枳椇子混合物对小鼠急性酒精中毒醒酒作用的研究[J].药学实践杂志,2017,35(5):398-401,410.
[7]刘明,钟赣生,陈绍红,等.中药防治酒精性肝损伤的实验研究概述[J].中华中医药杂志,2014,29(7):2270-2273.
[8]徐华容,蔡雪洲,袁鑫,等.甘露醇对急性酒精中毒小鼠行为学的影响[J].湖北科技学院学报:医学版,2015,29(1):4-5.
[9]王倩,唐东昕,龙奉玺,等.葛花解酲汤防治“酒伤病”的研究概况[J].中国民族民间医药,2017,26(5):67-69.
[10]高万露,汪小海.急性酒精中毒药物治疗新进展[J].药学与临床研究,2015,23(1):59-61.
[11]杨牧祥,于文涛,胡金宽.酒速愈对急性酒精中毒小鼠海马Ach E和纹状体单胺神经递质含量的影响[J].北京中医药大学学报,2007,30(2):112-114,123.
[12]刘彦明,胡忠心.251例慢性酒精中毒所致精神障碍临床分析[J].中国健康心理学杂志,2007,15(6):488-489.
[13]杨晓华,张华峰,赖江华.中枢单胺类神经递质在酒精依赖中的分子作用机制[J].遗传,2014,36(1):11-20.
[14]熊峰,周毅,赵娟,等.神经递质及其受体在酒精诱导神经系统损伤中的作用[J].神经损伤与功能重建,2014,9(3):243-245.
[15]王俊,刘建萍.酒精所致精神障碍的观察研究[J].中国社区医师:医学专业,2013,15(4):31-32.
[16]王旭,陈绍红,钟赣生,等.葛花、枳蓂子配伍对慢性酒精性肝损伤大鼠海马7种单胺类神经递质及代谢产物含量水平的影响[J].中国现代中药,2016,18(5):558-562,594.
[17]刘明,陈绍红,钟赣生,等.葛花枳椇子配伍对酒精性肝损伤大鼠肝脏功能及病理形态的影响[J].南京中医药大学学报,2015,31(2):147-152.
[18]柳海艳,钟赣生,李怡文,等.醇提和水提葛花枳椇子及其配伍对酒精性肝损伤大鼠肝脏抗氧化功能的影响[J].中华中医药杂志,2012,27(4):1181-1184.
[19]潘敏,谢少龙,丘慧秋,等.枳椇子、葛花的最佳配比解酒方影响人体乙醇吸收代谢过程研究[J].中国执业药师,2016,13(3):23-26.