陇南红提葡萄茎段组织培养研究
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摘要
以红提葡萄单芽茎段为外植体,研究了不同的消毒时间对葡萄外植体萌发的影响,确定最佳消毒时间,初代培养、继代增殖培养、生根培养以及组培苗驯化移栽等过程的条件。结果表明:使用75%乙醇消毒60s,0.1%氯化汞消毒8min,可以达到最佳灭菌效果,最佳初代培养基为DKW培养基+蔗糖30g·L~(-1)+琼脂8g·L~(-1)+6-BA0.2mg·L~(-1)+IAA0.2mg·L~(-1),生根培养基为1/2改良DKW培养基+IBA0.5mg·L~(-1)+NAA0.5mg·L~(-1)。
We used red grape stem with single bud as explants to study effect of different times of disinfection on their germination and to determine the best sterilization time needed.The tissue culture proceeded initial culture,secondary proliferation culture,root culture and seedling transplanting.The results showed that 75% ethanol for 60 sor 0.1% mercuric chloride for 8 min can achieve the best effect.The best original culture medium is DKW medium +sucrose 30 g·L~(-1)+agar 8 g·L~(-1)+6-BA0.2 mg·L~(-1)+IAA0.2 mg·L~(-1),and the rooting medium is 1/2 improved DKW medium+IBA0.5 mg·L~(-1)+NAA0.5 mg·L~(-1).
We used red grape stem with single bud as explants to study effect of different times of disinfection on their germination and to determine the best sterilization time needed.The tissue culture proceeded initial culture,secondary proliferation culture,root culture and seedling transplanting.The results showed that 75% ethanol for 60 sor 0.1% mercuric chloride for 8 min can achieve the best effect.The best original culture medium is DKW medium +sucrose 30 g·L~(-1)+agar 8 g·L~(-1)+6-BA0.2 mg·L~(-1)+IAA0.2 mg·L~(-1),and the rooting medium is 1/2 improved DKW medium+IBA0.5 mg·L~(-1)+NAA0.5 mg·L~(-1).
引文
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[2]张广波,王辉.组织培养技术对于葡萄的积极作用[J].现代园艺,2016(6):151-152.
[3]崔鹏,高秀宇.葡萄组织培养中愈伤组织的诱导[J].湖北农业科学,2012,5(4):827-830.
[4]修德仁.鲜食葡萄栽培与保鲜技术大全[M].北京:中国农业出版社,2004.
[5]宾宇波,沙海峰,任建武,等.百花山葡萄组织培养和快速繁殖[J].西北林学院学报,2013,28(6):99-102.
[6]徐美隆,刘静,王巧珍,等.酿酒葡萄“赤霞珠”组织培养快繁技术优化[J].北方园艺,2016(22):117-120.
[7]李国树,范国树,邱璐,等.水晶葡萄茎尖组织培养研究[J].中国南方果树,2010,39(2):66-67.
[8]张福庆,于向君,薛俊.酒用葡萄组培脱毒快繁技术的研究[J].中外葡萄与葡萄酒,2001,2(1):14-16.
[9]敬东,张丽,马洪爱,等.宁夏三个葡萄品种高效再生体系的建立[J].北方园艺,2011(22):118-121.
[10]沈传进,王利民.鲜食葡萄组织培养快速繁育系统的建立[J].河北农业科学,2011,15(7):16-21.
[11]高志明,闻杰.红提葡萄组织培养的研究[J].北方园艺,2011(9):144-145.
[12]高金辉,王玲,张厚良,等.山葡萄组织培养方法[J].东北林业大学学报,2007,35(7):37-39.