甘草油的质量标准研究
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摘要
目的:探讨甘草油的质量标准,为临床甘草油的合理使用提供依据与参考。方法:采用薄层鉴别法(TLC)对甘草油进行鉴别,根据《中国药典》中有关方法对甘草油进行常规检查,设定甘草苷作为对比。采用HPLC法测定甘草油中甘草苷的药物含量;采用紫外分光光度法测定甘草油中总黄酮的含量。结果:本研究中对甘草油进行6次测量,在不同选择溶液及参比溶液,将其放置在337 nm部位完成吸光度值A的测定,结果表明:甘草油中总黄酮含量存在明显的差异,均集中在13.2096～20.0451 mg,平均值为1.81;精密称取10μl甘草油放入液相色谱仪中,利用外标法测定甘草油中甘草苷及甘草酸含量,结果表明:不同试验下甘草油中甘草苷与甘草酸含量存在明显的差异性,甘草苷含量主要集中在0.1143～0.3743 mg,甘草酸含量为5.5433～6.3571 mg。结论:本研究起草的甘草油质量标准更加贴近我国现状,具有操作简单、方法准确、可靠等优点,具备良好的专属性、准确性,能有效的控制甘草油药材质量,值得应用。
Objective:To investigate the quality standards of licorice oil and provide basis and reference for the rational use of clinical licorice oil.Methods:The licorice oil was identified by thin layer identification(TLC) method.According to the relevant methods in the Chinese pharmacopoeia,the licorice oil was routinely inspected and glycyrrhizin was set as a comparison.The content of drug in licorice oil and glycyrrhizin was determined by HPLC.The content of total flavonoids in licorice oil was determined by UV spectrophotometry.Results:In this study,six measurements of licorice oil were performed.The absorbance values A were determined at 337 nm for different selected solutions and reference solutions.The results showed that the flavonoid content in licorice oil was significantly different under different absorbances.The differences were all concentrated between 13.2096 and 20.0451 mg with an average of 1.81%;10 μl of licorice oil was precisely weighed into the liquid chromatograph and the content of glycyrrhizin and glycyrrhizic acid in the licorice oil was determined by external standard method.The results showed that:Under the experiment,the content of glycyrrhizin and glycyrrhizic acid in licorice oil was obviously different.The content of glycyrrhizin was mainly concentrated in 0.1143-0.3743 mg and the content of glycyrrhizic acid was 5.5433-6.3571 mg.Conclusion:The licorice oil quality standard drafted in this study is more closely related to the status quo in China.It has the advantages of simple operation,accurate and reliable methods,good specificity and accuracy,and can effectively control the quality of licorice oil and medicinal materials,and is worthy of popularization and application.
Objective:To investigate the quality standards of licorice oil and provide basis and reference for the rational use of clinical licorice oil.Methods:The licorice oil was identified by thin layer identification(TLC) method.According to the relevant methods in the Chinese pharmacopoeia,the licorice oil was routinely inspected and glycyrrhizin was set as a comparison.The content of drug in licorice oil and glycyrrhizin was determined by HPLC.The content of total flavonoids in licorice oil was determined by UV spectrophotometry.Results:In this study,six measurements of licorice oil were performed.The absorbance values A were determined at 337 nm for different selected solutions and reference solutions.The results showed that the flavonoid content in licorice oil was significantly different under different absorbances.The differences were all concentrated between 13.2096 and 20.0451 mg with an average of 1.81%;10 μl of licorice oil was precisely weighed into the liquid chromatograph and the content of glycyrrhizin and glycyrrhizic acid in the licorice oil was determined by external standard method.The results showed that:Under the experiment,the content of glycyrrhizin and glycyrrhizic acid in licorice oil was obviously different.The content of glycyrrhizin was mainly concentrated in 0.1143-0.3743 mg and the content of glycyrrhizic acid was 5.5433-6.3571 mg.Conclusion:The licorice oil quality standard drafted in this study is more closely related to the status quo in China.It has the advantages of simple operation,accurate and reliable methods,good specificity and accuracy,and can effectively control the quality of licorice oil and medicinal materials,and is worthy of popularization and application.
引文
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[11] 刘琳琪,谭向宇,赵晨曦,等.大黄-栀子药对配伍比例和提取工艺对大黄中蒽醌类成分溶出的影响[J].中草药,2017,48(2):283-287.
[12] 颜永刚,尹立敏,王红艳,等.大黄-桃仁药对不同配比对大黄中10种成分提取量的影响[J].中药材,2016,39(7):1578-1581.
[13] Oaks BM,Laugero KD,Stewart CP,et al.Late-pregnancy salivary cortisol concentrations of ghanaian women participating in a randomized controlled trial of prenatal Lipid-based nutrient supplements[J].Journal of Nutrition,2016,146(2):343.
[14] 董红娇,陈晓虎,曾锐.UPLC-Q-Exactive四级杆-静电场轨道阱高分辨质谱联用快速分析民族药小大黄的化学成分[J].中草药,2016,47(14):2428-2435.
[15] 马喆,赵珺睿,董冉冉,等.基于高内涵分析技术的何首乌提取物及其主要成分肝毒性研究[J].中草药,2016,47(22):4021-4029.
[2] 王小宁,杨黎彬,张存劳.复方沙棘片的制备和质量标准研究[J].食品研究与开发,2016,37(14):75-79.
[3] 梁莹莹,张淑君,赫军,等.痛块消口服液的质量标准研究[J].中国药房,2017,28(3):374-376.
[4] Olivieri I,DAttino RM,Gualberti G,et al.THU0398 observational study on the quality of life of italian axial spondyloarthritis patients (QUASAR):Baseline data[J].Annals of the Rheumatic Diseases,2016,75(Suppl 2):332-333.
[5] 司席席,孙洪胜.复方金芩片的质量标准研究[J].中国药房,2016,27(3):415-417.
[6] 魏菱,陈虎,周永胜,等.数字化全口义齿个别托盘制作与临床应用时间评价[J].北京大学学报:医学版,2017,49(1):86-91.
[7] 王武,程英升,李明华,等.Willis覆膜支架治疗颅内段颈内动脉病变临床应用[J].介入放射学杂志,2016,25(3):185-188.
[8] 关健,曹晓瑞,王刚,等.多模式血液管理策略在初次单侧膝、髋关节置换术中的临床应用[J].中华解剖与临床杂志,2017,22(2):116-120.
[9] Jiang Z,Wang Y,Zheng Y,et al.Ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry and chemometric analysis of licorice based on the simultaneous determination of saponins and flavonoids[J].Journal of Separation Science,2016,39(15):2928-2940.
[10] 王亮,郭威,周倩,等.基于固相萃取和内标特征图谱的大黄质量评价方法[J].中药材,2016,39(6):1327-1332.
[11] 刘琳琪,谭向宇,赵晨曦,等.大黄-栀子药对配伍比例和提取工艺对大黄中蒽醌类成分溶出的影响[J].中草药,2017,48(2):283-287.
[12] 颜永刚,尹立敏,王红艳,等.大黄-桃仁药对不同配比对大黄中10种成分提取量的影响[J].中药材,2016,39(7):1578-1581.
[13] Oaks BM,Laugero KD,Stewart CP,et al.Late-pregnancy salivary cortisol concentrations of ghanaian women participating in a randomized controlled trial of prenatal Lipid-based nutrient supplements[J].Journal of Nutrition,2016,146(2):343.
[14] 董红娇,陈晓虎,曾锐.UPLC-Q-Exactive四级杆-静电场轨道阱高分辨质谱联用快速分析民族药小大黄的化学成分[J].中草药,2016,47(14):2428-2435.
[15] 马喆,赵珺睿,董冉冉,等.基于高内涵分析技术的何首乌提取物及其主要成分肝毒性研究[J].中草药,2016,47(22):4021-4029.