微波法提取木鳖子中丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的工艺研究
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摘要
目的:建立木鳖子中丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的微波提取方法,为木鳖子药材的进一步开发利用提供实验依据。方法:实验采用微波提取技术,以丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯含量为指标,考察乙醇浓度、微波功率、提取时间及提取温度对提取工艺的影响。结果:丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的线性范围为0.024~0.120 mg,平均加样回收率为100.2%。微波提取的最佳提取工艺为35%乙醇65℃,400 w提取12 min,平均提取率为0.267%。结论:微波法提取木鳖子中丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯的方法可靠稳定,优选出的提取工艺简单、节能、省时可行。
Objective: To establish the microwave extraction method of gypsogenin-3-O-glucuronide from momordica cochinchinensis, and provide the experimental basis for the further development of momordica cochinchinensis. Methods: Using microwave extraction technology, we studied the effects of ethanol concentration,microwave power, extraction time, extraction temperature on extraction process of gypsogenin-3-O-glucuronide.Results: Linear range of gypsogenin-3-O-glucuronide was 0.024 ~0.120 mg, and the average recovery rate was 100.2%. The best extraction process was to extract 12 minutes with 35% ethanol, 65 ℃ and 400 w. The average extraction rate was 83.4%. Conclusion: The microwave extraction method was reliable and stable to extract the gypsogenin-3-O-glucuronide from momordica cochinchinensis. At the same time, this optimized extraction process was simple, energy conversation, time-saving and feasible.
Objective: To establish the microwave extraction method of gypsogenin-3-O-glucuronide from momordica cochinchinensis, and provide the experimental basis for the further development of momordica cochinchinensis. Methods: Using microwave extraction technology, we studied the effects of ethanol concentration,microwave power, extraction time, extraction temperature on extraction process of gypsogenin-3-O-glucuronide.Results: Linear range of gypsogenin-3-O-glucuronide was 0.024 ~0.120 mg, and the average recovery rate was 100.2%. The best extraction process was to extract 12 minutes with 35% ethanol, 65 ℃ and 400 w. The average extraction rate was 83.4%. Conclusion: The microwave extraction method was reliable and stable to extract the gypsogenin-3-O-glucuronide from momordica cochinchinensis. At the same time, this optimized extraction process was simple, energy conversation, time-saving and feasible.
引文
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[2]林慧彬,彭相君,路俊仙,等.木鳖子的本草考证[J].四川中医,2009,27(2):54-56.
[3]姜仁辉.木鳖子皂苷分离纯化、鉴定及活性研究[D].济南:山东大学,2009:5-7.
[4]王秀琴,白宗利.木鳖子研究进展[J].辽宁中医药大学学报,2007,9(2):56-57.
[5]赵二劳,郭青枝,赵德千,等.微波辅助提取葛根素的研究[J].时珍国医国药,2009,20(2):321-322.
[6]郭振库,金钦汉,范国强,等.黄芩中黄芩苷微波提取的实验研究[J].中草药,2001,32(11):985-988.
[7]李辉,马书伟,刘艳,等.基于抗炎药效实验优选苦豆子提取工艺[J].北京中医药大学学报,2016,39(1):67-72.
[8]李凤英,王英姿,王晴,等.正交试验法优化珠子参总皂苷提取工艺[J].北京中医药大学学报,2016,39(2):151-155.
[9]周茆,石晓朦,王正宽,等.正交试验优化杏贝止咳提取物的微波干燥工艺[J].现代药物与临床,2016,31(2):143-148.
[10]欧阳丽娜,李兰林,吴雪,等.正交设计优选竹节参总皂苷微波提取工艺的研究[J].中草药,2010,41(10):1639-1642.
[11]龚志华,梁丹,张志博,等.微波辅助提取茶籽皂苷的研究[J].茶叶科学,2013,33(4):358-363.