不同的萃取方式分析葫芦巴浸膏挥发性成分
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摘要
为了明确葫芦巴浸膏中挥发性成分组成、含量及不同萃取方式对葫芦巴浸膏挥发性成分萃取效果的影响,分别采用固相微萃取(SPME)、溶剂萃取(LLE)和同时蒸馏萃取(SDE)对葫芦巴浸膏中挥发性成分进行提取,利用GC-MS技术对挥发性成分进行分析鉴定。3种萃取方法共鉴定出160种挥发性成分,其中固相微萃取(SPME)37种、溶剂萃取(LLE)66种、同时蒸馏萃取(SDE)113种,3种方式共有的16种,具有葫芦巴浸膏特征香味成分的葫芦巴内酯3种方式均能萃取出来,比较3种萃取方法可知:同时蒸馏萃取(SDE)和溶剂萃取(LLE)对葫芦巴浸膏中香味物质提取的更为全面,两种萃取方式共鉴定出151种。
In order to clarify the composition and content of volatile components in the fenugreek extract and the effect of different extraction methods on the extraction of volatile components of fenugreek extract,the volatile components in the fenugreek extract were extracted by solid phase microextraction(SPME),solvent extraction(LLE) and simultaneous distillation extraction(SDE),and the volatile components were analyzed and identified by GC-MS.A total of 160 volatile components were identified by three extraction methods,including 37 solid phase microextraction(SPME),66 solvent extraction(LLE),and 113 kinds of simultaneous distillation(SDE).The fenugreek lactone characteristic of the fenugreek extract can be extracted in three ways.Comparing the three extraction methods,it can be known that the simultaneous extraction(SDE) and solvent extraction(LLE) extract the aroma from the fenugreek extract more comprehensive,and a total of 151 species were identified by two extraction methods.
In order to clarify the composition and content of volatile components in the fenugreek extract and the effect of different extraction methods on the extraction of volatile components of fenugreek extract,the volatile components in the fenugreek extract were extracted by solid phase microextraction(SPME),solvent extraction(LLE) and simultaneous distillation extraction(SDE),and the volatile components were analyzed and identified by GC-MS.A total of 160 volatile components were identified by three extraction methods,including 37 solid phase microextraction(SPME),66 solvent extraction(LLE),and 113 kinds of simultaneous distillation(SDE).The fenugreek lactone characteristic of the fenugreek extract can be extracted in three ways.Comparing the three extraction methods,it can be known that the simultaneous extraction(SDE) and solvent extraction(LLE) extract the aroma from the fenugreek extract more comprehensive,and a total of 151 species were identified by two extraction methods.
引文
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[5]苏勇,冒德寿,曲荣芬,等.搅拌棒磁子萃取-热脱附-气相色谱质谱联用分析葫芦巴浸膏的挥发性成分[J].食品工业科技,2012,33(13):78-80.
[6]JU S K,KANG S S,CHOI J S,et al.Antioxidant components from aralia continentalis[J].Korean J.Pharmacogn.,1998,29(1):13-87.
[7]YOSHIKAWA M,MURAKAMI T,KOMATSU H,et al.Medicinal foodstuffs.Ⅳ.Fenugreek seed.(1):structures of trigoneosides Ⅰa,Ⅰb,Ⅱa,Ⅱb,Ⅲa,and Ⅲb,new furostanol saponins from the seeds of Indian Trigonella foenum-graecum L[J].Chem.Pharm.Bull.,1997,45(1):81-87.
[8]谢剑平.烟草香原料[M].北京:化学工业出版社,2009.
[9]李汉超,王淑娴.烟草、烟气化学及分析[M].郑州:河南科学技术出版社,1991.
[10]黄伟.SDE/GC-MS法对两种不同葫芦巴浸膏香气成分的分析[J].上海应用技术学院学报(自然科学版),2005,(4):318-321.
[11]廖素兰,翁器林,唐云云,等.不同萃取方式分析武夷水仙茶挥发性成分[J].食品工业科技,2017,38(12):7-16.
[12]陈燕,李小惠,张丹,等.不同萃取方式结合GC-MS对本格烧酒中风味物质的分析[J].食品工业科技,2016,37(12):60-66.
[13]侯鹏娟,郭鹏,茹琦,等.黄葵籽中挥发性成分的GC-MS分析研究[J].食品科技,2017,42(2):281-285.
[14]龙园园,杨秀芳,吴妍,等.同时蒸馏萃取法提取花椒挥发油工艺的优化[J].中成药,2018,40(9):2 076-2 079.
[15]张岚,李连元,李淑敏,等.固相微萃取检验技术应用及影响因素分析[J].中国公共卫生,2005,21(6):746-747.