Annexin A7在高癌家系鼻咽癌人群中的表达水平
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摘要
目的检测膜联蛋白A7(Annexin A7)在高癌家系鼻咽癌组织中的表达情况,以期探讨高癌家系鼻咽癌患者与Annexin A7之间的关系。方法采集门诊患者鼻咽组织标本(高癌家系鼻咽癌组织标本、散发性鼻咽癌组织标本及鼻咽黏膜慢性炎症患者组织标本各15例),应用RT-PCR及Western blot检测Annexin A7在各组标本的mRNA及蛋白质水平的表达情况。结果在高癌家系鼻咽癌患者、散发性鼻咽癌患者及鼻咽黏膜慢性炎症患者鼻咽组织中,Annexin A7 mRNA的相对表达量依次为0.828±0.046、0.459±0.048、0.312±0.072,各组间差异有统计学意义(P<0.05)。在高癌家系鼻咽癌患者、散发性鼻咽癌患者及鼻咽黏膜慢性炎症患者鼻咽组织中,Annexin A7蛋白相对表达量依次为0.867±0.068、0.624±0.065、0.392±0.087,各组间差异有统计学意义(P<0.05)。Annexin A7蛋白在鼻咽癌组织中的表达与TNM分期及淋巴结转移有关,与患者的年龄﹑性别无关。结论 Annexin A7在高癌家系鼻咽癌患者鼻咽组织中表达明显上调。Annexin A7的表达和肿瘤的分期和淋巴结转移有关。
Objective To investigate the expressions of the Annexin A7 in familial nasopharyngeal carcinoma(NPC), and the significance of Annexin A7 in familial NPC. Methods The clinical and pathological data of 15 familial NPC, 15 sporadic NPC and 15 chronic inflammation of nasopharyngeal mucosa cases were collected. RT-PCR and Western blot were applied to assess the expressions of Annexin A7 on the mRNA level and the protein level. Results The relative expressions of Annexin A7 mRNA in familial NPC, sporadic NPC and chronic inflammation patients were 0.828±0.046, 0.459±0.048 and 0.312±0.072, respectively, with significant difference among the 3 groups(P<0.05). The relative expressions of Annexin A7 in familial NPC, sporadic NPC and chronic inflammation patients were 0.867±0.068, 0.624±0.065 and 0.392±0.087, respectively, with significant difference among the 3 groups(P<0.05). The expression of Annexin A7 in NPC was correlated with tumor stage and lymph node metastasis. Conclusions The expression of Annexin A7 is up-regulated in familial NPC and correlated with tumor stage and lymph node metastasis.
Objective To investigate the expressions of the Annexin A7 in familial nasopharyngeal carcinoma(NPC), and the significance of Annexin A7 in familial NPC. Methods The clinical and pathological data of 15 familial NPC, 15 sporadic NPC and 15 chronic inflammation of nasopharyngeal mucosa cases were collected. RT-PCR and Western blot were applied to assess the expressions of Annexin A7 on the mRNA level and the protein level. Results The relative expressions of Annexin A7 mRNA in familial NPC, sporadic NPC and chronic inflammation patients were 0.828±0.046, 0.459±0.048 and 0.312±0.072, respectively, with significant difference among the 3 groups(P<0.05). The relative expressions of Annexin A7 in familial NPC, sporadic NPC and chronic inflammation patients were 0.867±0.068, 0.624±0.065 and 0.392±0.087, respectively, with significant difference among the 3 groups(P<0.05). The expression of Annexin A7 in NPC was correlated with tumor stage and lymph node metastasis. Conclusions The expression of Annexin A7 is up-regulated in familial NPC and correlated with tumor stage and lymph node metastasis.
引文
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[22] Ji H,Moritz RL,Kim YS,et al.Analysis of Ras-induced oncogenic transformation of NIH-3T3 cells using differential-display 2-DE proteomics[J].Electrophoresis,2007,28(12):1997-2008.
[23] 张华宋,陈舒华,张继平,等.F-肌动蛋白在鼻咽癌不同家族史患者中的表达[J].广东医学,2016,37(10):1501-1504.
[24] 陈舒华,刘丹,张继平,等.不同家族史鼻咽癌患者癌组织Prohibitin的表达[J].中国中西医结合耳鼻咽喉科杂志,2016,24(2):84-88.
[25] 杨民正,梁庆模.鼻咽癌CT表现与Annexin A7蛋白表达关系的研究[J].中国临床医学影像杂志,2011,22(1):6-9.
[2] Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61(2):69-90.
[3] Wee JT,Ha TC,Loong SL,et al.Is nasopharyngeal cancer really a "Cantonese cancer" [J].Chin J Cancer,2010,29(5):517-526.
[4] Ren ZF,Liu WS,Qin HD,et al.Effect of family history of cancers and environmental factors on risk of nasopharyngeal carcinoma in Guangdong,China[J].Cancer Epidemiol,2010,34(4):419-424.
[5] Huang SF,Hsiao JH,Young CK,et al.Familial aggregation of nasopharyngeal carcinoma in Taiwan[J].Oral Oncol,2017,73:10-15.
[6] Bei JX,Jia WH,Zeng YX.Familial and large-scale case-control studies identify genes associated with nasopharyngeal carcinoma[J].Semin Cancer Biol,2012,22(2):96-106.
[7] Chang ET,Adami HO.The enigmatic epidemiology of nasopharyngeal carcinoma[J].Cancer Epidemiol Biomarkers Prev,2006,15(10):1765-1777.
[8] Loh KS,Goh BC,Lu J,et al.Familial nasopharyngeal carcinoma in a cohort of 200 patients[J].Arch Otolaryngol Head Neck Surg,2006,132(1):82-85.
[9] 贾卫华,许宗利,冯炳健,等.广东鼻咽癌高危家族成员发病危险性的流行病学研究[J].癌症,2004,22(7):767-770.
[10] 陈舒华.鼻咽癌高癌家系体质证型的蛋白质组学研究[D].长沙:湖南中医药大学,2008.
[11] Caohuy H,Pollard HB.Activation of annexin 7 by protein kinase C in vitro and in vivo[J].J Biol Chem,2001,276(16):12813-12821.
[12] Srivastava M,Torosyan Y,Raffeld M,et al.ANXA7 expression represents hormone-relevant tumor suppression in different can-cers[J].Int J Cancer,2007,121(12):2628-2636.
[13] 潘建基.鼻咽癌2008分期介绍[C].2009′海峡两岸医药卫生交流与合作会议、海峡两岸立体定向肿瘤放射治疗技术论坛,北京:2009.
[14] Creutz CE,Pazoles CJ,Pollard HB.Identification and purification of an adrenal medullary protein (synexin) that causes calcium-dependent aggregation of isolated chromaffin granules[J].J Biol Chem,1978,253(8):2858-2866.
[15] Pollard HB,Burns AL,Rojas E.Synexin,a new member of the annexin gene family,is a calcium channel and membrane fusion protein[J].Prog Clin Biol Res,1990,349:159-172.
[16] Bai L,Guo Y,Du Y,et al.47kDa isoform of Annexin A7 affecting the apoptosis of mouse hepatocarcinoma cells line[J].Biomed Pharmacother,2016,83:1127-1131.
[17] Ye W,Li Y,Fan L,et al.Effect of annexin A7 suppression on the apoptosis of gastric cancer cells[J].Mol Cell Biochem,2017,429(1/2):33-43.
[18] Yuan HF,Li Y,Zhao Q,et al.Expression of annexin A7 and its clinical significance in differentiation and metastasis of gastric carcinoma[J].Int J Clin Exp Pathol,2014,7(10):6567-6574.
[19] Torosyan Y,Simakova O,Naga S,et al.Annexin-A7 protects normal prostate cells and induces distinct patterns of RB-associated cytotoxicity in androgen-sensitive and -resistant prostate cancer cells[J].Int J Cancer,2009,125(11):2528-2539.
[20] Srivastava M,Bubendorf L,Raffeld M,et al.Prognostic impact of ANX7-GTPase in metastatic and HER2-negative breast cancer patients[J].Clin Cancer Res,2004,10(7):2344-2350.
[21] Huang Y,Wang H,Yang Y.Annexin A7 is correlated with better clinical outcomes of patients with breast cancer[J].J Cell Biochem,2018,119(9):7577-7584.
[22] Ji H,Moritz RL,Kim YS,et al.Analysis of Ras-induced oncogenic transformation of NIH-3T3 cells using differential-display 2-DE proteomics[J].Electrophoresis,2007,28(12):1997-2008.
[23] 张华宋,陈舒华,张继平,等.F-肌动蛋白在鼻咽癌不同家族史患者中的表达[J].广东医学,2016,37(10):1501-1504.
[24] 陈舒华,刘丹,张继平,等.不同家族史鼻咽癌患者癌组织Prohibitin的表达[J].中国中西医结合耳鼻咽喉科杂志,2016,24(2):84-88.
[25] 杨民正,梁庆模.鼻咽癌CT表现与Annexin A7蛋白表达关系的研究[J].中国临床医学影像杂志,2011,22(1):6-9.