5种山榄科果树的耐寒性研究
|
摘要
【目的】低温是热带果树北移的关键限制因素,寒害会导致它们的产量下降甚至引种失败。对5种热带珍稀山榄科果树幼苗的耐寒性进行研究,为其引种驯化及推广种植提供理论依据。【方法】以2 a(年)苗龄实生苗的古巴牛乳树[Manilkara roxburghiana(Wight)Dubard]、人心果[Manilkara zapota(L.)van Royen]、神秘果(Synsepalum dulcif-cum Denill)、蛋黄果(Lucuma nervosa A. DC.)和星苹果(Chrysophyllum cainito L.)为试材,于光照培养箱中先9℃处理72 h,后3℃处理24 h。测定低温胁迫下叶片的叶绿素含量、相对电导率(REC)、半致死温度(LT50)、游离脯氨酸含量、可溶性蛋白含量、可溶性糖含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性等指标的变化。【结果】随着温度下降,5种果树的叶绿素含量不断降低,在3℃时皆显著低于对照;脯氨酸含量不断上升,在9℃、3℃均明显高于对照;可溶性蛋白含量先升后降,除人心果在3℃时和对照没有显著差异,其他4种在9℃、3℃时均显著高于对照;可溶性糖含量在9℃时达到峰值,此时5者之间有显著差异,其中古巴牛乳树最高,星苹果最低;MDA含量不断上升,到3℃时,与对照相比,只有古巴牛乳树、人心果没有显著升高,其他3种均有显著升高;SOD、POD及CAT活性均先升后降,在9℃时达到峰值,古巴牛乳树、人心果SOD、POD活性总体上显著高于其他3种,古巴牛乳树的CAT活性显著高于其他4种,星苹果最低。【结论】低温胁迫下,5种山榄科果树叶片各指标的变化趋势大体一致,但变化程度各有不同,耐寒性强弱顺序为:古巴牛乳树>人心果>神秘果>蛋黄果>星苹果。脯氨酸的含量和升幅,可溶性蛋白、可溶性糖的含量及SOD、POD、CAT的活性可以作为山榄科果树耐寒性评价的重要参考指标。
【Objective】Cold temperature is a key limit of northward expansion of the cultivation of trop-ical fruit trees. Chilling injure often causes yield reduction and even crop failure. Previous studies aboutSapotaceae fruit trees focused mainly on the description of commercial value and the prospects for devel-opment, but there have been few reports about the mechanisms of response to cold temperature whenthey were introduced northward. In this study. The semi-lethal temperature(LT50), as well as the chang-es in activities of antioxidant enzymes and the contents of osmoregulation substances in 5 Sapotaceaespecies under cold stress were studied in order to provide a theoretical basis for introduction and plant-ing these species.【Methods】Two-year-old grafted seedlings of five Sapotaceae species including Ma-nilkara roxburghiana(Wight) Dubard, Manilkara zapota(L.) van Royen, Synsepalum dulcifcum Denill,Lucuma nervosa A. DC. and Chrysophyllum cainito L. were placed in incubators for low temperaturetreatments. With 25 ℃ as the control, the experiment set two temperature treatments. First 9 ℃ for 72 h,and then 3 ℃ for 24 h. Mature leaves were collected immediately after every treatments and divided in-to two parts. One was used to determine chlorophylls and relative electric conductivity(REC); the otherwas stored at-80 ℃ for the analyses of the parameters including contents of proline, soluble proteins,soluble sugars and malondialdehyde(MDA), and the activities of superoxide dismutase(SOD), peroxi-dase activity(POD) and catalase(CAT). REC was determined following DENG Renju. LT50 was mea-sured according to LIU Shihong. Chlorophylls were measured using acetone-ethanol mixing method.POD, CAT, soluble sugars and MDA were determined following Zhang Zhiliang and Qu Weijing, whileSOD, proline and soluble proteins were analyzed using the methods of Li Hesheng's.【Results】The chlo-rophyll values of the 5 species kept decreasing as the temperature dropped. The range of decrease inchlorophylls was the largest in S. dulcifcum and lowest in M. roxburghiana and M. zapota. The value at3 ℃ in every specie was significantly lower than in the control. REC values in the 5 species increasedbut the rate of increase were not the same. The values in roxburghiana, M. zapota. and C. cainito at 9 ℃or at 3 ℃ were not significantly higher than their control values. A rapid rise was found in C. cainito at9 ℃ and in S. dulcifcum at 3 ℃. The REC value in M. roxburghiana or M. zapota was significantly low-er than the other 3 species. Regressed logistic equations between REC and temperature were established.The LT50 was calculated from the equations. The cold resistance based on LT50 was in the order of M. rox-burghiana(3.01)>M. zapota(3.32)>S. dulcifcum(3.36)>L. nervosa(3.80) >C. cainito(4.11). Pro-line content increased constantly and was significantly higher at 9 ℃or at 3 ℃ than at the control temper-ature. The content and the range of increase in M. roxburghiana and M. zapota. were higer than in theother 3 species. The lowest proline content was found in C. cainito. Soluble protein content increasedfirst at 9 ℃ and then decreased gradually at 3 ℃. Except for M. zapota, the protein values in other 4 spe-cies were significantly higher than the control either at 9℃or at 3 ℃. Soluble sugar content at either 9 ℃or 3 ℃ in every specie was higher than in the control. It rose rapidly and reached the peak at 9 ℃ whenthe value in every specie significantly differed from each other. The highest value was in M. roxburghi-ana and the lowest value in C. cainito. MDA increased constantly with the drop of temperature. It wassignificantly higher at 3℃ than the control except for M. roxburghiana and M. zapota. The activities ofSOD, POD or CAT in all specie showed a similar pattern with the decrease in temperature. The valuerose first and reached its peak values at 9 ℃, and then declined at 3 ℃. M. roxburghiana and M. zapotamaintained significantly higher SOD and POD activities than the other three species at all the low tem-peratures. CAT activity in M. roxburghiana was distinctly higher than that in the other species.【Conclu-sion】Chlorophyll content in all specie was lower under chilling stress than in the control. The values ofMDA, REC, proline, soluble proteins and soluble sugars were higher under cold stress compared withthe control. On the whole, the change patterns of parameter were similar among the five species undercold stress. Based on the changes in osmoregulation substance, antioxidant enzymes and LT50,the cold re-sistance was in the order of M. roxburghiana>M. zapota>S. dulcifcum>L. nervosa>C. cainito. Thecold resistance of the two M. species were obviously higher than the other three. The indexes includingthe content and the increase range of proline, the contents of soluble proteins or soluble sugars, the activi-ties of SOD, POD and CAT might be used to measure the cold resistance of Sapotaceae fruit trees.
【Objective】Cold temperature is a key limit of northward expansion of the cultivation of trop-ical fruit trees. Chilling injure often causes yield reduction and even crop failure. Previous studies aboutSapotaceae fruit trees focused mainly on the description of commercial value and the prospects for devel-opment, but there have been few reports about the mechanisms of response to cold temperature whenthey were introduced northward. In this study. The semi-lethal temperature(LT50), as well as the chang-es in activities of antioxidant enzymes and the contents of osmoregulation substances in 5 Sapotaceaespecies under cold stress were studied in order to provide a theoretical basis for introduction and plant-ing these species.【Methods】Two-year-old grafted seedlings of five Sapotaceae species including Ma-nilkara roxburghiana(Wight) Dubard, Manilkara zapota(L.) van Royen, Synsepalum dulcifcum Denill,Lucuma nervosa A. DC. and Chrysophyllum cainito L. were placed in incubators for low temperaturetreatments. With 25 ℃ as the control, the experiment set two temperature treatments. First 9 ℃ for 72 h,and then 3 ℃ for 24 h. Mature leaves were collected immediately after every treatments and divided in-to two parts. One was used to determine chlorophylls and relative electric conductivity(REC); the otherwas stored at-80 ℃ for the analyses of the parameters including contents of proline, soluble proteins,soluble sugars and malondialdehyde(MDA), and the activities of superoxide dismutase(SOD), peroxi-dase activity(POD) and catalase(CAT). REC was determined following DENG Renju. LT50 was mea-sured according to LIU Shihong. Chlorophylls were measured using acetone-ethanol mixing method.POD, CAT, soluble sugars and MDA were determined following Zhang Zhiliang and Qu Weijing, whileSOD, proline and soluble proteins were analyzed using the methods of Li Hesheng's.【Results】The chlo-rophyll values of the 5 species kept decreasing as the temperature dropped. The range of decrease inchlorophylls was the largest in S. dulcifcum and lowest in M. roxburghiana and M. zapota. The value at3 ℃ in every specie was significantly lower than in the control. REC values in the 5 species increasedbut the rate of increase were not the same. The values in roxburghiana, M. zapota. and C. cainito at 9 ℃or at 3 ℃ were not significantly higher than their control values. A rapid rise was found in C. cainito at9 ℃ and in S. dulcifcum at 3 ℃. The REC value in M. roxburghiana or M. zapota was significantly low-er than the other 3 species. Regressed logistic equations between REC and temperature were established.The LT50 was calculated from the equations. The cold resistance based on LT50 was in the order of M. rox-burghiana(3.01)>M. zapota(3.32)>S. dulcifcum(3.36)>L. nervosa(3.80) >C. cainito(4.11). Pro-line content increased constantly and was significantly higher at 9 ℃or at 3 ℃ than at the control temper-ature. The content and the range of increase in M. roxburghiana and M. zapota. were higer than in theother 3 species. The lowest proline content was found in C. cainito. Soluble protein content increasedfirst at 9 ℃ and then decreased gradually at 3 ℃. Except for M. zapota, the protein values in other 4 spe-cies were significantly higher than the control either at 9℃or at 3 ℃. Soluble sugar content at either 9 ℃or 3 ℃ in every specie was higher than in the control. It rose rapidly and reached the peak at 9 ℃ whenthe value in every specie significantly differed from each other. The highest value was in M. roxburghi-ana and the lowest value in C. cainito. MDA increased constantly with the drop of temperature. It wassignificantly higher at 3℃ than the control except for M. roxburghiana and M. zapota. The activities ofSOD, POD or CAT in all specie showed a similar pattern with the decrease in temperature. The valuerose first and reached its peak values at 9 ℃, and then declined at 3 ℃. M. roxburghiana and M. zapotamaintained significantly higher SOD and POD activities than the other three species at all the low tem-peratures. CAT activity in M. roxburghiana was distinctly higher than that in the other species.【Conclu-sion】Chlorophyll content in all specie was lower under chilling stress than in the control. The values ofMDA, REC, proline, soluble proteins and soluble sugars were higher under cold stress compared withthe control. On the whole, the change patterns of parameter were similar among the five species undercold stress. Based on the changes in osmoregulation substance, antioxidant enzymes and LT50,the cold re-sistance was in the order of M. roxburghiana>M. zapota>S. dulcifcum>L. nervosa>C. cainito. Thecold resistance of the two M. species were obviously higher than the other three. The indexes includingthe content and the increase range of proline, the contents of soluble proteins or soluble sugars, the activi-ties of SOD, POD and CAT might be used to measure the cold resistance of Sapotaceae fruit trees.
引文
[1]李丽纯,陈福梓,王加义,陈惠,林晶,李丽容.基于GIS的台湾青枣在福建引扩种的气候适宜性区划[J].中国生态农业学报,2017,25(1):47-54.LI Lichun,CHEN Fuzi,WANG Jiayi,CHEN Hui,LIN Jing,LILirong. Climate suitability regionalization for Taiwan green ju-jube introduction and expansion in Fujian province using GIS[J]. Chinese Journal of Eco-Agriculture,2017,25(1):47-54.
[2]杨为海,邹明宏,曾辉,万继锋,张汉周,石胜友,陆超忠.模拟低温对龙眼果实发育及其果皮H2O2代谢的影响[J].热带作物学报,2017,38(3):432-437.YANG Weihai,ZOU Minghong,ZENG Hui,WAN Jifeng,ZHANG Hanzhou,SHI Shengyou,LU Chaozhong. Effects ofsimulated low development and its temperature on longan fruitperiearp H2O2metabolism[J]. Chinese Journal of Tropical Crops,2017,38(3):432-437.
[3]常晓晓,陆育生,林志雄,潘建平,邱继水,唐振强.枇杷幼果雨雪冻害的理化响应[J].中国农学通报,2017,33(31):68-73.CHANG Xiaoxiao,LU Yusheng,LIN Zhixiong,PAN Jianping,QIU Jishui,TANG Zhenqiang. The physiochemical response ofyoung loquat fruit to freeze injury[J]. Chinese Agricultural Sci-ences Bulletin,2017,33(31):68-73.
[4]李运合,李玉生,张金梅,陈晓玲,赵艳华,吴永杰.芒果细胞超低温保存存活机理[J].植物生理学报,2016,52(10):1474-1480.LI Yunhe,LI Yusheng,ZHANG Jinmei,CHEN Xiaoling,ZHAOYanhua,WU Yongjie. The survival mechanism of mango cellsduring cryopreservation[J]. Plant Physiology Journal,2016,52(10):1474-1480.
[5]华敏,范鸿雁,张治礼,何凡,王祥和,胡福初.不良气候条件对海南杧果反季节生产的影响及预防措施[J].中国热带农业,2013(1):16-18.HUA Min,FAN Hongyan,ZHANG Zhili,HE Fan,WANGXianghe,HU Fuchu. Effect and precaution of bad weather condi-tion to counter-season production in Hainan[J]. China TropicalAgriculture,2013(1):16-18.
[6]刘冰浩,徐宁,朱建华,梁文,彭宏祥.黄江流广西龙眼种质耐寒性的CTR值鉴定[J].西南农业学报,2006,19(4):668-671.LIU Binghao,XU Ning,ZHU Jianhua,LIANG Wen,PENGHongxiang. The CTR identification of chilling-tolerance of lon-gan germplasm in Guangxi[J]. Southwest China Journal of Agri-cultural Sciences,2006,19(4):668-671.
[7]董万鹏,龙秀琴,代丽华,孙立坤,李培林,杨雨嘉.越冬期间西番莲低温半致死温度及越冬表现研究[J].核农学报,2016,30(8):1656-1663.DONG Wanpeng,LONG Xiuqin,DAI Lihua,SUN Likun,LIPeilin,YANG Yujia. Study on the semi-lethal temperature andwintering performance of Passiflora edulis in verwintering[J].Journal of Nuclear Agricultural Sciences,2016,30(8):1656-1663.
[8]邓仁菊,范建新,王永清,金吉芬,刘涛.火龙果幼苗对低温胁迫的生理响应及其抗寒性综合评价[J].植物生理学报,2014,50(10):1529-1534.DENG Renju,FAN Jianxin,WANG Yongqing,JIN Jifen,LIUTao. Physiological responses of pitaya(Hylocereus spp.)seed-lings to chilling stress and comprehensive evaluation of theircold resistance[J]. Plant Physiology Journal,2014,50(10):1529-1534.
[9]刘育梅,金亮,郑国华,邱静瑜,梁诗.苏拉威西凤果叶片在不同低温胁迫下的生理特性变化[J].福建农业学报,2017,32(2):167-170.LIU Yumei,JIN Liang,ZHENG Guohua,QIU Jingyu,LIANGShi. Changes of physiological characteristics in Garcinia celebi-ca L. leaves under different low temperature stress[J]. FujianJournal of Agricultural Sciences,2017,32(2):167-170.
[10]刘育梅.山榄科(Sapotaceae)果树在NaCl胁迫下的适应生理、组织结构变化及差异蛋白研究[D].厦门:厦门大学,2011.LIU Yumei. The physiological adaptability,changes in tissuestructure and differentially expressed proteins of Sapotaceae spe-cies under NaCl stress[D]. Xiamen:Xiamen University,2011.
[11]刘育梅,宋志瑜,池敏杰,叶婷,卢昌义.五种山榄科果树叶片组织结构与耐盐性的相关性[J].厦门大学学报,2017,56(2):300-304.LIU Yumei,SONG Zhiyu,CHI Minjie,YE Ting,LU Changyi.Correlation research on leaf anatomy of 5 Sapotaceae speciesand their salt tolerance[J]. Journal of Xiamen University,2017,56(2):300-304.
[12]刘育梅,胡宏友,童庆宣,李学梅,池敏杰,卢昌义. NaCl胁迫对两种铁线子属果树叶片生理特性的影响[J].热带作物学报,2011,32(9):1679-1682.LIU Yumei,HU Hongyou,TONG Qingxuan,LI Xuemei,CHIMinjie,LU Changyi. Effect of NaCl stress on physiological char-acteristics of two Manilkara species leaves[J]. Chinese Joumalof Tropical Crops,2011,32(9):1679-1682.
[13]谭才邓,李静,邓毛程.神秘果果实总RNA提取方法比较研究[J].基因组学与应用生物学,2013,32(3):409-412.TAN Caideng,LI Jing,DENG Maocheng. Study on total RNAextraction methods from miracle fruit[J]. Genomics and AppliedBiology,2013,32(3):409-412.
[14]黄巨波,刘红,卢圣楼,张浩.神秘果种子蛋白质的提取与降糖效用研究[J].天然产物研究与开发,2012,24(10):1441-1443.HUANG Jubo,LIU Hong,LU Shenglou,ZHANG Hao. Studyon hypoglycemic activity and extraction of Synsepalum dulcifi-cum seed protein[J]. Natural Product Research and Develop-ment,2012,24(10):1441-1443.
[15] LU S L,LIU H,CHEN G Y. Chemical composition of leaf essen-tial oil of synsepalum dulcificum and evaluation of its antibacte-rial and antitumoral activities in vitro[J]. Chemistry and Industryof Forest Products,2014,34(1):121-127.
[16] MEIRA N A,ROCHA L W,DA SILVA G F,QUINTAL Z M,MONACHE D F D,FILHO V C,QUINT?O N L. Chrysophyl-lum cainito leaves are effective against pre-clinical chronic painmodels:Analysis of crude extract,fraction and isolated com-pounds in mice[J]. Journal of Ethnopharmacology,2016,184:30-41.
[17] MAO L M,QI X W,HAO J H,LIU H F,XU Q H,BU P L. Invitro,ex vivo and in vivo anti-hypertensive activity of Chryso-phyllum cainito L. extract[J]. International Journal of Clinicaland Experimental Medicine,2015,8(10):17912-17922.
[18]魏秀清,余东,章希娟,许玲,陈志峰,张丽梅,许家辉. 6个杧果品种耐寒性的研究[J].果树学报,2017,34(5):594-602.WEI Xiuqing,YU Dong,ZHANG Xijuan,XU Ling,CHEN Zhi-feng,ZHANG Limei,XU Jiahui. A study on cold resistance insix mango cultivars[J]. Journal of Fruit Science,2017,34(5):594-602.
[19]王小媚,唐文忠,任惠,方位宽,苏伟强,刘业强,陆贵峰.水杨酸对低温胁迫番木瓜幼苗生理指标及叶片组织结构的影响[J].南方农业学报,2016,47(8):1290-1296.WANG Xiaomei,TANG Wenzhong,REN Hui,FANG Weikuan,SU Weiqiang,LIU Yeqiang,LU Guifeng. Effects of salicylic ac-id on physiological index and tissue structure of Carica papayaL. seedling under cold stress[J]. Journal of Southern Agricul-ture,2016,47(8):1290-1296.
[20] LAO D L C,SHIESH C C,LIN H L. Determination of chillingsen-sitivity of mango(Mangifera indica L.)leaves using chloro-phyll fluorescence[J]. Horticulture Nchu,2010,35(4):23-32.
[21]黄馨莹.杧果栽培种耐逆境温度筛选指标的建立[D].台中:中兴大学,2011.HUANG Xinying. Establishing screening methods and indicesfor tolerance under stress temperatures in mango(Magifera indi-ca L.)[D]. Taichung:National Chung Hsing University,2011.
[22]邓仁菊,范建新,王永清,金吉芬,刘涛.低温胁迫下火龙果的半致死温度及抗寒性分析[J].植物生理学报,2014,50(11):1742-1748.DENG Renju,FAN Jianxin,WANG Yongqing,JIN Jifen,LIUTao. Semilethal temperature of pitaya under low temperaturestress and evaluation on their cold resistance[J]. Plant Physiolo-gy Journal,2014,50(11):1742-1748.
[23]刘世红,田耀华,魏丽萍,周会平.西双版纳30个橡胶树品种的低温半致死温度及低温对抗氧化系统的影响[J].植物生理学报,2011,47(5):505-511.LIU Shihong,TIAN Yaohua,WEI Liping,ZHOU Huiping.Semi-lethal low temperatures and impact of low temperature onantioxidant system of 30 varieties of rubber trees in Xishuang-banna[J]. Plant Physiology Journal,2011,47(5):505-511.
[24]张治安,陈展宇.植物生理实验学技术[M].长春:吉林大学出版社,2008:68-87.ZHANG Zhi’an,CHEN Zhanyu. Science and technology ofplant physiology experiment[M]. Changchun:Jilin UnivercityPress,2008:68-87.
[25]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003:268-272.ZHANG Zhiliang,QU Weijing. The experimental guide forplant physiology[M]. 3rd ed. Beijing:Higher Education Press,2003:268-272.
[26]李合生.现代植物生理学[M].北京:高等教育出版社,2001:415-420.LI Hesheng. Modern plant physiology[M]. Beijing:Higher Edu-cation Press,2001:415-420.
[27]魏秀清,许玲,章希娟,余东,陈志峰,许家辉.莲雾对低温胁迫的生理响应及抗寒性分析[J].果树学报,2016,33(1):73-80.WEI Xiuqing,XU Ling,ZHANG Xijuan,YU Dong,CHEN Zhi-feng,XU Jiahui. Physiological responses of wax apple to lowtemperature and its cold resistance[J]. Journal of Fruit Science,2016,33(1):73-80.
[28]王小华,庄南生.脯氨酸与植物抗寒性的研究进展[J].中国农学通报,2008,24(11):398-402.WANG Xiaohua,ZHUANG Nansheng. Advances in research onproline and cold resistance of plant[J]. Chinese Agricultural Sci-ence Bulletin,2008,24(11):398-402.
[29]张保青,杨丽涛,李杨瑞.自然条件下甘蔗品种抗寒生理生化特性的比较[J].作物学报,2011,37(3):496-505.ZHANG Baoqing,YANG Litao,LI Yangrui. Comparison of phy-siological and biochemical characteristics related to cold resis-tance in sugarcane under field conditions[J]. Acta AgronomicaSinca,2011,37(3):496-505.
[30]杨惠,翟梅枝,李丽,许静,王丹,王振元,肖志娟.不同核桃品种的耐寒性及其渗透调节机制[J].西北植物学报,2013,33(10):2003-2009.YANG Hui,ZHAI Meizhi,LI Li,XU Jing,WANG Dan,WANGZhenyuan,XIAO Zhijuan. Cold-tolerance and osmoregulationmechanism of walnut cuitivars[J]. Acta Botanica Boreali-Occi-dentalia Sinica,2013,33(10):2003-2009.
[31]王振波,陈疏影,李智勇,李富生.甘蔗苗期对低温的响应及其耐寒性评价[J].中国农学通报,2015,31(30):162-168.WANG Zhenbo,CHEN Shuying,LI Zhiyong,LI Fusheng. Re-sponse to low temperature stress and evaluation for its cold resis-tance at sugarcane seedling stage[J]. Chinese Agricultural Sci-ence Bulletin,2015,31(30):162-168.
[32]相昆,张美勇,徐颖,王晓芳,岳林旭.不同核桃品种特性综合评价[J].应用生态学报,2011,22(9):2325-2330.XIANG Kun,ZHANG Meiyong,XU Ying,WANG Xiaofang,YUE Linxu. Cold-tolerance of walnut cultivars:a comprehen-sive evaluation[J]. Chinese Journal of Applied Ecology,2011,22(9):2325-2330.
[33]张静会,齐永顺,陈崇,王凤春,王同坤,张京政.低温对燕山板栗枝条生理特性的影响研究[J].中国农学通报,2012,28(16):65-68.ZHANG Jinghui,QI Yongshun,CHEN Chong,WANG Feng-chun,WANG Tongkun,ZHANG Jingzheng. Influence of tem-perature on the physiological characteristics of Yanshan chestnutbranches[J]. Chinese Agricultural Science Bulletin,2012,28(16):65-68.
[34]姜慧,徐迎春,李永荣,翟敏.香橼不同品系性的研究[J].园艺学报,2012,39(3):525-532.JIANG Hui,XU Yingchun,LI Yongrong,ZHAI Min. The studyon cold resistance of different Citrus medica strains[J]. ActaHorticulturae Sinica,2012,39(3):525-532.
[2]杨为海,邹明宏,曾辉,万继锋,张汉周,石胜友,陆超忠.模拟低温对龙眼果实发育及其果皮H2O2代谢的影响[J].热带作物学报,2017,38(3):432-437.YANG Weihai,ZOU Minghong,ZENG Hui,WAN Jifeng,ZHANG Hanzhou,SHI Shengyou,LU Chaozhong. Effects ofsimulated low development and its temperature on longan fruitperiearp H2O2metabolism[J]. Chinese Journal of Tropical Crops,2017,38(3):432-437.
[3]常晓晓,陆育生,林志雄,潘建平,邱继水,唐振强.枇杷幼果雨雪冻害的理化响应[J].中国农学通报,2017,33(31):68-73.CHANG Xiaoxiao,LU Yusheng,LIN Zhixiong,PAN Jianping,QIU Jishui,TANG Zhenqiang. The physiochemical response ofyoung loquat fruit to freeze injury[J]. Chinese Agricultural Sci-ences Bulletin,2017,33(31):68-73.
[4]李运合,李玉生,张金梅,陈晓玲,赵艳华,吴永杰.芒果细胞超低温保存存活机理[J].植物生理学报,2016,52(10):1474-1480.LI Yunhe,LI Yusheng,ZHANG Jinmei,CHEN Xiaoling,ZHAOYanhua,WU Yongjie. The survival mechanism of mango cellsduring cryopreservation[J]. Plant Physiology Journal,2016,52(10):1474-1480.
[5]华敏,范鸿雁,张治礼,何凡,王祥和,胡福初.不良气候条件对海南杧果反季节生产的影响及预防措施[J].中国热带农业,2013(1):16-18.HUA Min,FAN Hongyan,ZHANG Zhili,HE Fan,WANGXianghe,HU Fuchu. Effect and precaution of bad weather condi-tion to counter-season production in Hainan[J]. China TropicalAgriculture,2013(1):16-18.
[6]刘冰浩,徐宁,朱建华,梁文,彭宏祥.黄江流广西龙眼种质耐寒性的CTR值鉴定[J].西南农业学报,2006,19(4):668-671.LIU Binghao,XU Ning,ZHU Jianhua,LIANG Wen,PENGHongxiang. The CTR identification of chilling-tolerance of lon-gan germplasm in Guangxi[J]. Southwest China Journal of Agri-cultural Sciences,2006,19(4):668-671.
[7]董万鹏,龙秀琴,代丽华,孙立坤,李培林,杨雨嘉.越冬期间西番莲低温半致死温度及越冬表现研究[J].核农学报,2016,30(8):1656-1663.DONG Wanpeng,LONG Xiuqin,DAI Lihua,SUN Likun,LIPeilin,YANG Yujia. Study on the semi-lethal temperature andwintering performance of Passiflora edulis in verwintering[J].Journal of Nuclear Agricultural Sciences,2016,30(8):1656-1663.
[8]邓仁菊,范建新,王永清,金吉芬,刘涛.火龙果幼苗对低温胁迫的生理响应及其抗寒性综合评价[J].植物生理学报,2014,50(10):1529-1534.DENG Renju,FAN Jianxin,WANG Yongqing,JIN Jifen,LIUTao. Physiological responses of pitaya(Hylocereus spp.)seed-lings to chilling stress and comprehensive evaluation of theircold resistance[J]. Plant Physiology Journal,2014,50(10):1529-1534.
[9]刘育梅,金亮,郑国华,邱静瑜,梁诗.苏拉威西凤果叶片在不同低温胁迫下的生理特性变化[J].福建农业学报,2017,32(2):167-170.LIU Yumei,JIN Liang,ZHENG Guohua,QIU Jingyu,LIANGShi. Changes of physiological characteristics in Garcinia celebi-ca L. leaves under different low temperature stress[J]. FujianJournal of Agricultural Sciences,2017,32(2):167-170.
[10]刘育梅.山榄科(Sapotaceae)果树在NaCl胁迫下的适应生理、组织结构变化及差异蛋白研究[D].厦门:厦门大学,2011.LIU Yumei. The physiological adaptability,changes in tissuestructure and differentially expressed proteins of Sapotaceae spe-cies under NaCl stress[D]. Xiamen:Xiamen University,2011.
[11]刘育梅,宋志瑜,池敏杰,叶婷,卢昌义.五种山榄科果树叶片组织结构与耐盐性的相关性[J].厦门大学学报,2017,56(2):300-304.LIU Yumei,SONG Zhiyu,CHI Minjie,YE Ting,LU Changyi.Correlation research on leaf anatomy of 5 Sapotaceae speciesand their salt tolerance[J]. Journal of Xiamen University,2017,56(2):300-304.
[12]刘育梅,胡宏友,童庆宣,李学梅,池敏杰,卢昌义. NaCl胁迫对两种铁线子属果树叶片生理特性的影响[J].热带作物学报,2011,32(9):1679-1682.LIU Yumei,HU Hongyou,TONG Qingxuan,LI Xuemei,CHIMinjie,LU Changyi. Effect of NaCl stress on physiological char-acteristics of two Manilkara species leaves[J]. Chinese Joumalof Tropical Crops,2011,32(9):1679-1682.
[13]谭才邓,李静,邓毛程.神秘果果实总RNA提取方法比较研究[J].基因组学与应用生物学,2013,32(3):409-412.TAN Caideng,LI Jing,DENG Maocheng. Study on total RNAextraction methods from miracle fruit[J]. Genomics and AppliedBiology,2013,32(3):409-412.
[14]黄巨波,刘红,卢圣楼,张浩.神秘果种子蛋白质的提取与降糖效用研究[J].天然产物研究与开发,2012,24(10):1441-1443.HUANG Jubo,LIU Hong,LU Shenglou,ZHANG Hao. Studyon hypoglycemic activity and extraction of Synsepalum dulcifi-cum seed protein[J]. Natural Product Research and Develop-ment,2012,24(10):1441-1443.
[15] LU S L,LIU H,CHEN G Y. Chemical composition of leaf essen-tial oil of synsepalum dulcificum and evaluation of its antibacte-rial and antitumoral activities in vitro[J]. Chemistry and Industryof Forest Products,2014,34(1):121-127.
[16] MEIRA N A,ROCHA L W,DA SILVA G F,QUINTAL Z M,MONACHE D F D,FILHO V C,QUINT?O N L. Chrysophyl-lum cainito leaves are effective against pre-clinical chronic painmodels:Analysis of crude extract,fraction and isolated com-pounds in mice[J]. Journal of Ethnopharmacology,2016,184:30-41.
[17] MAO L M,QI X W,HAO J H,LIU H F,XU Q H,BU P L. Invitro,ex vivo and in vivo anti-hypertensive activity of Chryso-phyllum cainito L. extract[J]. International Journal of Clinicaland Experimental Medicine,2015,8(10):17912-17922.
[18]魏秀清,余东,章希娟,许玲,陈志峰,张丽梅,许家辉. 6个杧果品种耐寒性的研究[J].果树学报,2017,34(5):594-602.WEI Xiuqing,YU Dong,ZHANG Xijuan,XU Ling,CHEN Zhi-feng,ZHANG Limei,XU Jiahui. A study on cold resistance insix mango cultivars[J]. Journal of Fruit Science,2017,34(5):594-602.
[19]王小媚,唐文忠,任惠,方位宽,苏伟强,刘业强,陆贵峰.水杨酸对低温胁迫番木瓜幼苗生理指标及叶片组织结构的影响[J].南方农业学报,2016,47(8):1290-1296.WANG Xiaomei,TANG Wenzhong,REN Hui,FANG Weikuan,SU Weiqiang,LIU Yeqiang,LU Guifeng. Effects of salicylic ac-id on physiological index and tissue structure of Carica papayaL. seedling under cold stress[J]. Journal of Southern Agricul-ture,2016,47(8):1290-1296.
[20] LAO D L C,SHIESH C C,LIN H L. Determination of chillingsen-sitivity of mango(Mangifera indica L.)leaves using chloro-phyll fluorescence[J]. Horticulture Nchu,2010,35(4):23-32.
[21]黄馨莹.杧果栽培种耐逆境温度筛选指标的建立[D].台中:中兴大学,2011.HUANG Xinying. Establishing screening methods and indicesfor tolerance under stress temperatures in mango(Magifera indi-ca L.)[D]. Taichung:National Chung Hsing University,2011.
[22]邓仁菊,范建新,王永清,金吉芬,刘涛.低温胁迫下火龙果的半致死温度及抗寒性分析[J].植物生理学报,2014,50(11):1742-1748.DENG Renju,FAN Jianxin,WANG Yongqing,JIN Jifen,LIUTao. Semilethal temperature of pitaya under low temperaturestress and evaluation on their cold resistance[J]. Plant Physiolo-gy Journal,2014,50(11):1742-1748.
[23]刘世红,田耀华,魏丽萍,周会平.西双版纳30个橡胶树品种的低温半致死温度及低温对抗氧化系统的影响[J].植物生理学报,2011,47(5):505-511.LIU Shihong,TIAN Yaohua,WEI Liping,ZHOU Huiping.Semi-lethal low temperatures and impact of low temperature onantioxidant system of 30 varieties of rubber trees in Xishuang-banna[J]. Plant Physiology Journal,2011,47(5):505-511.
[24]张治安,陈展宇.植物生理实验学技术[M].长春:吉林大学出版社,2008:68-87.ZHANG Zhi’an,CHEN Zhanyu. Science and technology ofplant physiology experiment[M]. Changchun:Jilin UnivercityPress,2008:68-87.
[25]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003:268-272.ZHANG Zhiliang,QU Weijing. The experimental guide forplant physiology[M]. 3rd ed. Beijing:Higher Education Press,2003:268-272.
[26]李合生.现代植物生理学[M].北京:高等教育出版社,2001:415-420.LI Hesheng. Modern plant physiology[M]. Beijing:Higher Edu-cation Press,2001:415-420.
[27]魏秀清,许玲,章希娟,余东,陈志峰,许家辉.莲雾对低温胁迫的生理响应及抗寒性分析[J].果树学报,2016,33(1):73-80.WEI Xiuqing,XU Ling,ZHANG Xijuan,YU Dong,CHEN Zhi-feng,XU Jiahui. Physiological responses of wax apple to lowtemperature and its cold resistance[J]. Journal of Fruit Science,2016,33(1):73-80.
[28]王小华,庄南生.脯氨酸与植物抗寒性的研究进展[J].中国农学通报,2008,24(11):398-402.WANG Xiaohua,ZHUANG Nansheng. Advances in research onproline and cold resistance of plant[J]. Chinese Agricultural Sci-ence Bulletin,2008,24(11):398-402.
[29]张保青,杨丽涛,李杨瑞.自然条件下甘蔗品种抗寒生理生化特性的比较[J].作物学报,2011,37(3):496-505.ZHANG Baoqing,YANG Litao,LI Yangrui. Comparison of phy-siological and biochemical characteristics related to cold resis-tance in sugarcane under field conditions[J]. Acta AgronomicaSinca,2011,37(3):496-505.
[30]杨惠,翟梅枝,李丽,许静,王丹,王振元,肖志娟.不同核桃品种的耐寒性及其渗透调节机制[J].西北植物学报,2013,33(10):2003-2009.YANG Hui,ZHAI Meizhi,LI Li,XU Jing,WANG Dan,WANGZhenyuan,XIAO Zhijuan. Cold-tolerance and osmoregulationmechanism of walnut cuitivars[J]. Acta Botanica Boreali-Occi-dentalia Sinica,2013,33(10):2003-2009.
[31]王振波,陈疏影,李智勇,李富生.甘蔗苗期对低温的响应及其耐寒性评价[J].中国农学通报,2015,31(30):162-168.WANG Zhenbo,CHEN Shuying,LI Zhiyong,LI Fusheng. Re-sponse to low temperature stress and evaluation for its cold resis-tance at sugarcane seedling stage[J]. Chinese Agricultural Sci-ence Bulletin,2015,31(30):162-168.
[32]相昆,张美勇,徐颖,王晓芳,岳林旭.不同核桃品种特性综合评价[J].应用生态学报,2011,22(9):2325-2330.XIANG Kun,ZHANG Meiyong,XU Ying,WANG Xiaofang,YUE Linxu. Cold-tolerance of walnut cultivars:a comprehen-sive evaluation[J]. Chinese Journal of Applied Ecology,2011,22(9):2325-2330.
[33]张静会,齐永顺,陈崇,王凤春,王同坤,张京政.低温对燕山板栗枝条生理特性的影响研究[J].中国农学通报,2012,28(16):65-68.ZHANG Jinghui,QI Yongshun,CHEN Chong,WANG Feng-chun,WANG Tongkun,ZHANG Jingzheng. Influence of tem-perature on the physiological characteristics of Yanshan chestnutbranches[J]. Chinese Agricultural Science Bulletin,2012,28(16):65-68.
[34]姜慧,徐迎春,李永荣,翟敏.香橼不同品系性的研究[J].园艺学报,2012,39(3):525-532.JIANG Hui,XU Yingchun,LI Yongrong,ZHAI Min. The studyon cold resistance of different Citrus medica strains[J]. ActaHorticulturae Sinica,2012,39(3):525-532.