新疆古尔班通古特沙漠不同地区荒漠肉苁蓉功效物质评价
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摘要
以新疆古尔班通古特沙漠中的荒漠肉苁蓉为材料,研究不同区域荒漠肉苁蓉中的总黄酮、多糖、可溶性蛋白质、微量元素(铜、铁、锰、锌)及松果菊苷和毛蕊花糖苷的含量及差异,旨在为评价和选育适合古尔班通古特沙漠生长且药用功效物质含量高的优良品种提供依据。结果显示,古尔班通古特沙漠不同区域荒漠肉苁蓉中的功效物质含量以芳草湖地区最高、达18.21%,其次为塔城地区的18.18%;荒漠肉苁蓉中活性成分含量最高的是总黄酮、平均含量达到6.868%,其次为松果菊苷、平均含量达2.718%;不同部位荒漠肉苁蓉中,肉质茎(下部)含功效物质最高,上部与中部的物质含量较低。综合评价古尔班通古特沙漠不同区域荒漠肉苁蓉的功效物质含量高低依次为芳草湖>塔城>奇台>吉木萨尔>博乐>阿勒泰,表明古尔班通古特沙漠中不同区域产荒漠肉苁蓉中活性成分含量存在一定程度的差异,芳草湖和塔城地区产荒漠肉苁蓉功效物质含量最高,肉质茎中的功效物质含量远高于上部及中部茎,说明下部肉质茎的药效更好、更具有开发利用前景。
Using Cistanche deserticola as the material, this paper studied contents and differences of the total flavonoids, polysaccharides, soluble proteins, trace elements(copper, iron, manganese, zinc), echinacoside and verbascoside in different regions of the Gurbantünggüt Desert. The purpose of this study was to provide a basis for the evaluation and selection of fine varieties which are suitable for the growth of Gurbantünggüt Desert with high content of medicinal substances. The results showed that the content of the efficacy of C. deserticola was up to 18.21% in the Fangcao Lake area. Followed by 18.18% in Tacheng area; The content of total flavonoids was the highest active ingredient in C. deserticola with an average 6.868%, next was echinacoside with 2.718%. The efficacy of C. deserticola was the highest in the fleshy stem(lower) and lower in the upper and middle parts. The effective content of C. deserticola was in the order of Fangcao Lake > Tacheng > Qitai > Jimsar > Bole > Altay in different regions of the Gurbantünggüt Desert by comprehensive evaluation. These results indicated that there were some differences in the active ingredients of C. deserticola in different regions of Gurbantünggüt Desert, the contents of effective substances of C. deserticola were the highest in Fangcao Lake and Tacheng area. The content of effective substances in the fleshy stem was much higher than that in upper and middle stem, indicating that the lower fleshy stem had better efficacy and the prospect of development and utilization.
Using Cistanche deserticola as the material, this paper studied contents and differences of the total flavonoids, polysaccharides, soluble proteins, trace elements(copper, iron, manganese, zinc), echinacoside and verbascoside in different regions of the Gurbantünggüt Desert. The purpose of this study was to provide a basis for the evaluation and selection of fine varieties which are suitable for the growth of Gurbantünggüt Desert with high content of medicinal substances. The results showed that the content of the efficacy of C. deserticola was up to 18.21% in the Fangcao Lake area. Followed by 18.18% in Tacheng area; The content of total flavonoids was the highest active ingredient in C. deserticola with an average 6.868%, next was echinacoside with 2.718%. The efficacy of C. deserticola was the highest in the fleshy stem(lower) and lower in the upper and middle parts. The effective content of C. deserticola was in the order of Fangcao Lake > Tacheng > Qitai > Jimsar > Bole > Altay in different regions of the Gurbantünggüt Desert by comprehensive evaluation. These results indicated that there were some differences in the active ingredients of C. deserticola in different regions of Gurbantünggüt Desert, the contents of effective substances of C. deserticola were the highest in Fangcao Lake and Tacheng area. The content of effective substances in the fleshy stem was much higher than that in upper and middle stem, indicating that the lower fleshy stem had better efficacy and the prospect of development and utilization.
引文
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[17]田建平,李娟玲,胡远艳,等.冬青属苦丁茶叶总黄酮含量测定与资源评价[J].食品科技,2014(1):278-281.
[18]刘惠东,李桂芳,牛亮仲,等.维药多伞阿魏中总黄酮提取工艺研究及含量测定[J].中华中医药杂志,2016,31(1):310-312.
[19]王静霞,黄艳菲,赵小燕,等.荞麦和商品苦荞茶中总黄酮的含量测定[J].食品工业科技,2013,34(2):58-60.
[20]陈敏,肖苏萍,黄璐琦,等.管花肉苁蓉中松果菊苷和毛蕊花糖苷的含量测定[J].中国中药杂志,2005(11):839-841.
[21]许欢欢,胡君萍,吴姗姗,等.肉苁蓉苯乙醇总苷微乳的制备及质量评价[J].中药新药与临床药理,2016(5):712-716.
[22]黄倩,尹智慧,盛振华,等.ICP-OES法测定9种产地玫瑰花中15种微量元素[J].浙江中医药大学学报,2015(3):217-220.
[23]陈华,董利森.肉苁蓉多糖抗衰老作用的研究进展[J].中国煤炭工业医学杂志,2012,15(2):310-311.
[2]马慧,尹若熙,郭敏,等.肉苁蓉多糖对D-半乳糖致衰老模型小鼠CREB表达的影响[J].中国实验方剂学杂志,2014,20(20):137-141.
[3]高晓霞,陈君,彭艳丽.肉苁蓉多糖药理作用研究概况[J].食品与药品,2015,17(2):136-139.
[4]薛海燕,焦婵媛,姚军.肉苁蓉总苷药理作用的研究现状[J].中国临床药理学杂志,2018,34(4):486-488.
[5]颜贵卉,田金虎,龙本文,等.肉苁蓉中苯乙醇苷类成分的研究进展[J].中南药学,2012,10(9):692-695.
[6]南泽东,任华忠,赵明波,等.HPLC-MS鉴定塔中栽培荒漠肉苁蓉药材的17个成分[J].中国实验方剂学杂志,2018,24(16):76-81.
[7]高云佳,姜勇,戴昉,等.肉苁蓉润肠通便的药效物质研究[J].中国现代中药,2015,17(4):307-310,314.
[8]国家药典委员会.中华人民共和国药典[S].北京:化学工业出版社,2010:126.
[9]Gu C, Yang X, Huang L. Cistanches herba:A neuropharmacology review. Front Pharmacol,2016, 7:289.
[10]杨坤,焦智浩,张根发.肉苁蓉组织培养研究进展及应用前景[J].中草药,2006,37(1):140-143.
[11]覃文婷,宿美凤,雒晓梅,等.定性分析不同产地荒漠肉苁蓉苯乙醇苷类及多糖类成分[J].辽宁中医药大学学报,2018,20(7):77-81.
[12]张继州.考马斯亮蓝法测定天花粉饮片水煎剂中蛋白质含量[J].亚太传统医药,2015,11(17):20-21.
[13]曲春香,沈颂东,王雪峰,等.用考马斯亮蓝法测定植物粗提液中可溶性蛋白质含量方法的研究[J].苏州大学学报(医学版),2006,22(2):82-85.
[14]王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009,23(3):40-42.
[15]夏永刚,梁军,杨炳友,等.苯酚-硫酸法测定麻黄多糖含量研究[J].中医药信息,2011,28(1):33-35.
[16]于瑞涛,朱鹏程,陶燕铎,等.苯酚硫酸法测定迷果芹多糖的含量[J].分析试验室,2008,27(Z2):222-224.
[17]田建平,李娟玲,胡远艳,等.冬青属苦丁茶叶总黄酮含量测定与资源评价[J].食品科技,2014(1):278-281.
[18]刘惠东,李桂芳,牛亮仲,等.维药多伞阿魏中总黄酮提取工艺研究及含量测定[J].中华中医药杂志,2016,31(1):310-312.
[19]王静霞,黄艳菲,赵小燕,等.荞麦和商品苦荞茶中总黄酮的含量测定[J].食品工业科技,2013,34(2):58-60.
[20]陈敏,肖苏萍,黄璐琦,等.管花肉苁蓉中松果菊苷和毛蕊花糖苷的含量测定[J].中国中药杂志,2005(11):839-841.
[21]许欢欢,胡君萍,吴姗姗,等.肉苁蓉苯乙醇总苷微乳的制备及质量评价[J].中药新药与临床药理,2016(5):712-716.
[22]黄倩,尹智慧,盛振华,等.ICP-OES法测定9种产地玫瑰花中15种微量元素[J].浙江中医药大学学报,2015(3):217-220.
[23]陈华,董利森.肉苁蓉多糖抗衰老作用的研究进展[J].中国煤炭工业医学杂志,2012,15(2):310-311.